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The Effects of Alendronate on Healing of the Calvarial Defect in Rats  

Kim, Jae-Hyung (Department of Periodontology College of Dentistry Kyungpook National University)
Lee, Jae-Mok (Department of Periodontology College of Dentistry Kyungpook National University)
Park, Jin-Woo (Department of Periodontology College of Dentistry Kyungpook National University)
Suh, Jo-Young (Department of Periodontology College of Dentistry Kyungpook National University)
Publication Information
Journal of Periodontal and Implant Science / v.34, no.4, 2004 , pp. 733-746 More about this Journal
Abstract
The present study aimed to examine the effects of topical application of alendronate with a collagen membrane on the healing of the calvarial defect in rats, which has a good experimental design for the healing of tissue destruction, To study the effect of alendronate on bone healing, the collagen membrane containing $200{\mu}g$ alendronate was inserted in the defects of the right side and collagen membrane treated with physiologic saline was inserted in the defects of the left side. After 1, 2 and 4 weeks, observation of histologic feature after H&E staining, cell counting after TRAP staining, and hardness measurement(Knoop) were performed. In histologic finding, similar features were shown for both test and control groups each week. In cell counting only the 1 week test groups showed significant reduction of TRAP(+)cells than control groups(p<0.01) and the control groups showed statistically significant difference for 1, 2, 4weeks(p<0.05). In hardness measurement, The 2 week test groups showed significant higher hardness than control groups.(p<0,05) and not 4 weeks. There was significant increase of hardness for both groups as time goes by.(p<0.0l) Therefore local application of alendronate with collagen membrane was somewhat effective in reducing osteoclastic activity and increasing hardness in the early stage of healing. Further investigation concerning the actual effect of alendronate for bony healing will be necessary to apply the clinical cases
Keywords
bisphosphonates; Alendronate;
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