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The effect of the Ca-P coated DBBP on osseous regeneration in the rat calvarial bone defect  

Sung, Sun-Ju (Department of Periodontology, College of Dentistry, Chonnam National University)
Chung, Hyun-Ju (Department of Periodontology, College of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University)
Park, Hong-Ju (Dental Science Research Institute, Chonnam National University)
Kim, Ok-Su (Department of Periodontology, College of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University)
Kim, Young-Jun (Department of Periodontology, College of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University)
Publication Information
Journal of Periodontal and Implant Science / v.34, no.3, 2004 , pp. 475-487 More about this Journal
Abstract
Purpose: This study was aimed to evaluate the effect of the deproteinated bovine bone powder (DBBP) coated with calcium phosphate (Ca-P) on osseous regeneration in the calvarial bone defect of rat. Materials and Methods : The DBBP (Control group, n=6) and the Ca-P coated DBBP (Experimental group, n=6) were grafted in the critical sized calvarial bone defect (8 mm) of rat weighing 250 g. The animals were sacrificed at 1, 4 week. The biopsy specimens were decalcified with 5%formaldehyde and embedded in paraffin. The rats were sacrificed at 8 week received tetracycline (1 week), calcein blue (4 week), and alizarin red (7 week), and the biopsy specimens were taken. The specimens were embedded in methylmethacrylate and ground to 10 ${\mu}m$ thin sections were made. All of the specimens were stained with H & E and Masson's trichrome and examined under light microscope. The specimens at 8 week were examined under fluorescent microscope. Results : In the Control group, the grafted DBBP was surrounded with connective tissue, and osteoblasts were observed partially around the grafted particles at 1 week. At 4 week, some osteoid was observed and, new bone formation was observed at the periphery of grafted materials at 8 week, In the Experimental group, some osteoid was seen at the periphery of the grafted Ca-P coated DBBP at 1 week, and osteoblast and newly formed bone were observed around the grafted materials. At 8 week, newly formed bone was observed at the periphery of the grafted materials. Conclusion: These results suggest that Ca-P coated DBBP group was more and faster than DBBP group in new bone formation and Ca-P could contribute to enhance bone formation in the critical sized calvarial bone defect of rat.
Keywords
Ca-P; DBBP; osseous regeneration; calvarial bone defect;
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