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http://dx.doi.org/10.14368/jdras.2022.38.3.150

A study on the effect of microgroove-fibronectin complex titanium plate on the expression of various cell behavior-related genes in human gingival fibroblasts  

Hwang, Yu Jeong (Department of Biomaterials & Prosthodontics, Kyung Hee University College of Dentistry, Kyung Hee Unoversity Hospital at Gangdong)
Lee, Won Joong (Department of Biomaterials & Prosthodontics, Kyung Hee University College of Dentistry, Kyung Hee Unoversity Hospital at Gangdong)
Leesungbok, Richard (Department of Biomaterials & Prosthodontics, Kyung Hee University College of Dentistry, Kyung Hee Unoversity Hospital at Gangdong)
Lee, Suk Won (Department of Biomaterials & Prosthodontics, Kyung Hee University College of Dentistry, Kyung Hee Unoversity Hospital at Gangdong)
Publication Information
Journal of Dental Rehabilitation and Applied Science / v.38, no.3, 2022 , pp. 150-161 More about this Journal
Abstract
Purpose: To determine the effects of the microgroove-fibronectin complex surface on the expression of various genes related to cellular activity in human gingival fibroblasts. Materials and Methods: Smooth titanium specimens (NE0), acid-treated titanium specimens (E0), microgroove and acid-treated titanium specimens (E60/10), fibronectin-fixed smooth titanium specimens (NE0FN), acid-treated and fibronectin-immobilized titanium specimens (E0FN), and microgroove and acid-treated titanium specimens immobilized with fibronectin (E60/10FN) were prepared. Real-time polymerase chain reaction experiments were conducted on 44 genes related to cell behavior of human gingival fibroblasts. Results: Adhesion and proliferation of human gingival fibroblast on microgroove-fibronectin complex titanium were activated through four types of signaling pathway. Integrin α5, Integrin β1, Integrin β3, Talin-2, which belong to the focal adhesion pathway, AKT1, AKT2, NF-κB, which belong to the PI3K-AKT signaling pathway, MEK2, ERK1, ERK2, which belong to the MAPK signaling pathway, and Cyclin D1, CDK4, CDK6 genes belonging to the cell cycle signaling pathway were upregulated on the microgroove-fibronectin complex titanium surface (E60/10FN). Conclusion: The microgroove-fibronectin complex titanium surface can up-regulate various genes involved in cell behavior.
Keywords
titanium; fibronectin; gingival fibroblasts; cell behavior; gene expression;
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