Browse > Article
http://dx.doi.org/10.14368/jdras.2015.31.3.212

Antifungal effect of electrolyzed hydrogen water on Candida albicans biofilm  

Pyo, Kyung-Ryul (Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University)
Yoo, Yun Seung (Dental Research Institute, Dankook University)
Baek, Dong-Heon (Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University)
Publication Information
Journal of Dental Rehabilitation and Applied Science / v.31, no.3, 2015 , pp. 212-220 More about this Journal
Abstract
Purpose: Candida albicans can cause mucosal disease in many vulnerable patients. Also they are associated with denture-related stomatitis. Electrolyzed water is generated by electric current passed via water using various metal electrodes and has antimicrobial activity. The aim of this study was to investigate antifungal activity of electrolyzed water on C. albicans biofilm. Materials and Methods: C. albicans was cultured by sabouraud dextrose broth and F-12 nutrient medium in aerobic and 5% $CO_2$ condition to form blastoconidia (yeast) and hyphae type, respectively. For formation of C. albicans biofilm, C. albicans was cultivated on rough surface 6-well plate by using F-12 nutrient medium in $CO_2$ incubator for 48 hr. After electrolyzing tap water using various metal electrodes, the blastoconidia and hyphal type of C. albicans were treated with electrolyzed water. C. albicans formed blastoconidia and hyphae type when they were cultured by sabouraud dextrose broth and F-12 nutrient medium, respectively. Results: The electrolyzed water using palladium electrode (EWP) exhibited antifungal effect on blastoconidia of C. albicans. Also, the EWP significantly has antifungal activity against C. albicans biofilm and hyphae. In the electrolyzed water using various metal electrodes, only the EWP have antifungal activity. Conclusion: The EWP may use a gargle solution and a soaking solution for prevention of oral candidiasis and denture-related stomatitis due to antifungal activity.
Keywords
electrolyzed water; antifungal activity; Candida albicans; Candidal biofilm;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Coronado-Castellote L, Jimenez-Soriano Y. Clinical and microbiological diagnosis of oral candidiasis. J Clin Exp Dent 2013;5:e279-86.
2 Rupp S. Interactions of the fungal pathogen Candida albicans with the host. Future Microbiol 2007;2:141-51.   DOI
3 Sudbery P, Gow N, Berman J. The distinct morphogenic states of Candida albicans. Trends Microbiol 2004;12:317-24.   DOI
4 Sevilla MJ, Odds FC. Development of Candida albicans hyphae in different growth media--variations in growth rates, cell dimensions and timing of morphogenetic events. J Gen Microbiol 1986;132:3083-8.
5 Calderone RA, Fonzi WA. Virulence factors of Candida albicans. Trends Microbiol 2001;9:327-35.   DOI
6 Bulad K, Taylor RL, Verran J, McCord JF. Colonization and penetration of denture soft lining materials by Candida albicans. Dent Mater 2004;20:167-75.   DOI
7 Huh JB, Lim Y, Youn HI, Chang BM, Lee JY, Shin SW. Effect of denture cleansers on Candida albicans biofilm formation over resilient liners. J Adv Prosthodont 2014;6:109-14.   DOI
8 Nikawa H, Hamada T, Yamashiro H, Kumagai H. A review of in vitro and in vivo methods to evaluate the efficacy of denture cleansers. Int J Prosthodont 1999;12:153-9.
9 Verran J, Maryan CJ. Retention of Candida albicans on acrylic resin and silicone of different surface topography. J Prosthet Dent 1997;77:535-9.   DOI
10 Kitamura T, Todo H, Sugibayashi K. Effect of several electrolyzed waters on the skin permeation of lidocaine, benzoic acid, and isosorbide mononitrate. Drug Dev Ind Pharm 2009;35:145-53.   DOI
11 Hricova D, Stephan R, Zweifel C. Electrolyzed water and its application in the food industry. J Food Prot 2008;71:1934-47.   DOI
12 Pangloli P, Hung YC. Efficacy of slightly acidic electrolyzed water in killing or reducing Escherichia coli O157:H7 on iceberg lettuce and tomatoes under simulated food service operation conditions. J Food Sci 2011;76:M361-6.   DOI
13 Ye J, Li Y, Hamasaki T, Nakamichi N, Komatsu T, Kashiwagi T, Teruya K, Nishikawa R, Kawahara T, Osada K, Toh K, Abe M, Tian H, Kabayama S, Otsubo K, Morisawa S, Katakura Y, Shirahata S. Inhibitory effect of electrolyzed reduced water on tumor angiogenesis. Biol Pharm Bull 2008;31:19-26.   DOI
14 Park SK, Park SK. Electrolyzed-reduced water increases resistance to oxidative stress, fertility, and lifespan via insulin/IGF-1-like signal in C. elegans. Biol Res 2013;46:147-52.   DOI
15 Lee SH, Choi BK. Antibacterial effect of electrolyzed water on oral bacteria. J Microbiol 2006;44:417-22.
16 Komachiya M, Yamaguchi A, Hirai K, Kikuchi Y, Mizoue S, Takeda N, Ito M, Kato T, Ishihara K, Yamashita S, Akihiro K. Antiseptic effect of slightly acidic electrolyzed water on dental unit water systems. Bull Tokyo Dent Coll 2014;55:77-86.   DOI
17 Rautemaa R, Ramage G. Oral candidosis--clinical challenges of a biofilm disease. Crit Rev Microbiol 2011;37:328-36.   DOI
18 Mayer FL, Wilson D, Hube B. Candida albicans pathogenicity mechanisms. Virulence 2013;4:119-28.   DOI
19 Goll G, Smith DE, Plein JB. The effect of denture cleansers on temporary soft liners. J Prosthet Dent 1983;50:466-72.   DOI
20 Sudbery PE. Growth of Candida albicans hyphae. Nat Rev Microbiol 2011;9:737-48.   DOI
21 Blankenship JR, Mitchell AP. How to build a biofilm: a fungal perspective. Curr Opin Microbiol 2006;9:588-94.   DOI
22 Tanaka H, Hirakata Y, Kaku M, Yoshida R, Takemura H, Mizukane R, Ishida K, Tomono K, Koga H, Kohno S, Kamihira S. Antimicrobial activity of superoxidized water. J Hosp Infect 1996;34:43-9.   DOI
23 Nakagawara S, Goto T, Nara M, Ozawa Y, Hotta K, Arata Y. Spectroscopic characterization and the pH dependence of bactericidal activity of the aqueous chlorine solution. Analytical Sciences 1998;14:691-8.   DOI
24 Nakajima N, Nakano T, Harada F, Taniguchi H, Yokoyama I, Hirose J, Daikoku E, Sano K. Evaluation of disinfective potential of reactivated free chlorine in pooled tap water by electrolysis. J Microbiol Methods 2004;57:163-73.   DOI