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http://dx.doi.org/10.11109/JAES.2014.20.2.77

Isolation and Characterization of Phosphorus Accumulating Microorganisms under Liquid Fertilization of Swine Slurry  

Lim, Joung-Soo (Animal Environment Division, National Institute of Animal Science, RDA)
Cho, Sung-Back (Animal Environment Division, National Institute of Animal Science, RDA)
Hwang, Ok-Hwa (Animal Environment Division, National Institute of Animal Science, RDA)
Yang, Seung-Hak (Animal Environment Division, National Institute of Animal Science, RDA)
Publication Information
Journal of Animal Environmental Science / v.20, no.2, 2014 , pp. 77-84 More about this Journal
Abstract
This study was conducted to investigate the bacterial capability to accumulate phosphorus during liquid composting process of pig slurry. Storage liquid compost and pig slurry were analyzed by using MALDI-TOF technique, which showed the colonies of Acinetobacter towneri and Bacillus licheniformis. In addition, bacterial colonies were isolated under high phosphoric acid conditions using X-phosphate MOPS medium with the addition of 2 mM $K_2HPO_4$. Microbial growth was observed in high and low phosphoric conditions due to the growth of bacterial diversities in the liquid fertilizer and slurry. The colonies isolated in the high phosphoric acid medium were uncultured bacterium clone and Acinetobacter sp. were identified by analysis of 16S rRNA gene sequences. Uncultured bacterium showed higher growth rate and excellent phosphorus ability then Acinetobacter sp.. In addition to Paenibacillus sp. AEY-1 isolated from pig slurry performed excellent phosphorus utilizing capability.
Keywords
Swine; Phosphorus; Accumulating; Microorganism; Liquid fertilizer;
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