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http://dx.doi.org/10.11109/JAES.2013.19.2.155

Effects of Cynanchum Wilfordii Extract on In vitro Ruminal Fermentation Characteristics and Methane Production  

Yang, Seung-Hak (National Institute of Animal Science, R.D.A.)
Lim, Joung-Soo (National Institute of Animal Science, R.D.A.)
Kim, Byul (National Institute of Animal Science, R.D.A.)
Hwang, Ok-Hwa (National Institute of Animal Science, R.D.A.)
Cho, Sung-Back (National Institute of Animal Science, R.D.A.)
Choi, Dong-Yoon (National Institute of Animal Science, R.D.A.)
Choi, Seok-Geun (Hankyong National University)
Hwang, Seong-Gu (Hankyong National University)
Publication Information
Journal of Animal Environmental Science / v.19, no.2, 2013 , pp. 155-162 More about this Journal
Abstract
The objective of this study is to investigate the effects of Cynanchum wilfordii (CW) on cell viability, anti-oxidant activity, volatile fatty acid (VFA) production and methane gas production. Collected rumen fluid incubated with CW powder (1% w/v) for 12 and 24 hours were analyzed for pH, VFAs and methane. Alamar blue assay showed no significant difference on the viability of 3T3-L1 and C2C12 cells treated with CW for 24 hours. TBARS data showed a dose dependent increase on the antioxidant activity of CW. VFAs increased in the CW-treated groups compared to the control group. In addition, propionate increased more than other VFAs by the treatment with CW. There was a significant decrease in methane gas production in batch culture treated with CW in 12hrs. In conclusion, it was suggested that Cynanchum wilfordii could manipulate rumen fermentation considered by increasing VFA production and inhibition of methanogenesis.
Keywords
Rumen fermentation; Cynanchum wilfordii; VFA; Methanogenesis;
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1 Ohkawa, H., Ohishi, N., Yagi, K., 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358.   DOI   ScienceOn
2 Ok, J.U., Lee, S.M., Lim, J.H., Lee, S.J., Moon, Y.H., Lee, S.S., 2006. Effects of Plant-origin Biological Active Materials on the Activities of Pathogenic Microbes and Rumen Microbes. J. Anim. Sci. & Technol. (Kor) 48, 709-718.   과학기술학회마을   DOI   ScienceOn
3 Van Nevel, C.J., Demeyer, D.I., 1977. Effect of monensin on rumen metabolism in vitro. Appl. Environ. Microbiol. 34, 251-257.
4 Ok, J.U., Baek, Y.Ch., Kim, K.H., Lee, S.Ch., Seol, Y.J., Lee, K.Y., Choi, Ch.W., Jeon, Ch.O., Lee, S.S., Lee, S.S., Oh., Y.K. 2011. Effects of Saponin Contained Plant Extracts on Ruminal Fermentation Characteristics and Methane Production. J. Anim. Sci. & Technol. (Kor) 53, 147-154.   과학기술학회마을   DOI   ScienceOn
5 Patra, A.K., Kamra, D.N., Agarwal, N., 2006. Effect of plant extract on in vitro mutagenesis, enzyme activity and fermentation of feed in rumen liquor of buffalo. Anim Feed Sci. Technol. 128, 276-291.   DOI   ScienceOn
6 Van Nevel, C.J., Demeyer, D.I., 1992. Influence of antibiotics and a deaminase inhibitor on volatile fatty acids and methane production from detergent washed hay and soluble starch by rumen microbes in vitro. Anim. Feed Sci. Technol. 37, 21-31.   DOI   ScienceOn
7 Whetstone, H.D., David, C.L., Bryant, M.P., 1981. Effect of monensin on breakdown of protein by ruminal microorganisms in vitro. J. Anim. Sci. 53, 803-809.
8 Yang, S.H., Lee, S.Y., Cho, S.B., Park, K.H., Park, J.K., Choi, D.Y., Yoo, Y.H., 2011. The Effect of Vegetable Sources Supplementation on in vitro Ruminal Methane Gas Production. Korea Soc. Livestock Housing Environ. (Kor) 17, 171-180.   과학기술학회마을
9 Blaxter, K.L., 1989. Energy Metabolism in Animals and Man. Cambridge University Press, New York. USA.
10 Carmean, B.R., 1991. Persistence of monensin effects on nutrient flux in steers. M.S. Thesis. Colorado State Univ., Fort Collins. USA.
11 Czerkawski, J.W., Blaxter, K.L., Wainman, F.W., 1966. The metabolism of oleic, linoleic, and linolenic acids by sheep with reference to their effects on methane production. Br. J. Nutr. 20, 349-362.
12 Erwin, E.S., Marco, J., Emery, E.M., 1961. Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44, 1768.   DOI
13 Mauricio, R.M., Mould, F.L., Dhanoa, M.S., Owen, E., Channa, K.S., Theodorou, M.K., 1999. A semi-automated in vitro gas production technique for ruminant feedstuff evaluation. Anim. Feed Sci. Tech. 79, 321-330.   DOI   ScienceOn
14 Frumholtz, P.P., Newbold, C.J., Wallace, R.J., 1989. Influence of Aspergillus oryzae fermentation extract on the fermentation of a basal ration in the rumen simulation technique (Rusitec). J. Agric. Sci. 113, 169-172.   DOI
15 Goering, H., Van Soest, K., 1970. Forages fiber analysis. Agricultural hand book no. 379. Department of Agriculture, Washington D.C. USA.
16 Machmuller, A., 2006. Medium-chain fatty acids and their potential to reduce methanogenesis in domestic ruminants. Agric. Ecosy. Environ. 112, 107-114.   DOI   ScienceOn
17 Nugent, J.H.A., Mangan., J.L., 1981. Characteristics of the rumen proteolysis of fraction I (18S) leaf protein from lucern (Medicago sativa L.). Br. J. Nutr. 46, 39-58.   DOI   ScienceOn
18 Official Methods of Analysis, 1995. Association of official analytical chemistry (AOAC) INTERNATIONAL, 16th Ed. Washington, D.C., USA.
19 National Inventory Report, 2013. Greenhouse Gas Inventory & Research Center of Korea.