Browse > Article
http://dx.doi.org/10.11109/JAES.2013.19.1.055

Callus Induction and Plant Regeneration Efficiency According to Tissue Culture Conditions in Teff grass (Eragrostis)  

Lee, Ki-Won (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Moon, Jin Young (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Park, Hyung Soo (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Choi, Gi Jun (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Kim, Ki-Yong (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Ji, Hee Chung (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Hwang, Tae Young (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Lee, Sang-Hoon (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Publication Information
Journal of Animal Environmental Science / v.19, no.1, 2013 , pp. 55-62 More about this Journal
Abstract
Teff grass is a warm season C4 annual grass that is used for dry hay, silage and haylage. We have developed a high-frequency plant regeneration system for teff grass via callus culture using mature seeds. It was revealed that mature seeds cultured on MS medium supplemented with 2 mg/l 2,4-D, 0.5 g/L proline, 0.5 g/L casamino acid and 3 g/L Gelrite under light condition produced the highest percentage of callus formation (91.9%). Addition of cytokinins (BA) at 0.0~0.5 mg/L to media containing 2 mg/l 2,4-D enhanced callus growth. The most suitable medium for plant regeneration from dehydrated calli was MS agar medium supplemented with 0.1 mg/l NAA, 1 mg/l BA, 0.5 g/L proline, 0.5 g/L casamino acid 3 g/L Gelrite which induced the highest percentage of calli forming shoots (47.0%). The shoots were rooted at the highest rate (100%) when transferred onto 1/2 MS medium and acclimated in greenhouse conditions.
Keywords
Callus; Regeneration; Teff grass; Tissue culture;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15, 473-497.   DOI
2 Schenk, R.U., Hildebrandt, A.C., 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot. 50, 199-204.   DOI
3 Sheibani, M., Azghandi, A., Nemati, S., 2007. Induction of somatic embryogenesis in Saffron using thidiazuron. Pakistan Journal of Biological Science. 10, 3564-3570.   DOI
4 Smale, M., Aquino, P., Crossa, J., del Toro, E., Dubin, J., Fischer, T., Fox, P., Khairallah, M., Mujeeb-Kazi, A., Nightingale, K., Ortiz-Monasterio, I., Rajaram, S., Singh, R., Skovmand, B., van Ginkel, M., Varughese, G., Ward, R., 1996. Understanding global trends in the use of wheat diversity and international flows of wheat genetic resources. Economics Working Paper 96-02. Mexico, D.F.: CIMMYT. 61pp.
5 Smith, R.L., Grando, M.F., Li Y.Y., Seib, J.C., Shatters, R.G., 2002. Transformation of bahiagrass (Paspalum notatum Flugge). Plant Cell Rep. 20, 10-1021.
6 Lee, K.-W., Kim, K.-Y., Choi, G.J., Lim, Y.C., Kim, W.H., Jung, M.W., Seo, S., Lee, B.-H., Lee, S.-H., 2008. Callus Induction and plant regeneration from mature seeds of timothy. J. Kor. Grassl. Forage Sci. 28, 165-170.   과학기술학회마을   DOI   ScienceOn
7 Assefa, K., Gaj, D., Maluszynski, M., 1998. Somatic embryogenesis and plant regeneration in mature seed culture of teff [Eragrostis tef (Zucc.) Trotter]. Plant Cell Reports. 18, 156-158.
8 Bekele, E., 1985. A review of research on diseases of barely, tef, and wheat in Ethiopia. pp. 79-108. In: Abate, T. (Ed.). A review of crop protection research in Ethiopia. Proceedings of the First Ethiopian Crop Prod. Symp. Dept. Crop protection, Institute of Agricultural Research, Addis Ababa, Ethiopia.
9 Chu, C.C., Wang, C.S., Sun, C.C., Hsu, C., Yin, K.C., Chu, C.Y., Bi, F.Y., 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic. 18, 659-668.
10 Ketema, S., 1997. Teff [Eragrostis tef (Zucc.) Trotter]. Promoting the conservation and use of underutilized and neglected crops. 12. Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome, Italy. 50pp.
11 Lee, K.-W., Lee, J.K., Kim, K.-Y., Ji, H.C., Park, H.S., Kim, K.-H., Lee, B.-H., Lee, S.-H., 2009a. Plant regeneration capacity of calluses derived from mature seed of perennial ryegrass cultivars. J. Kor. Grassl. Forage Sci. 29, 285-290.   과학기술학회마을   DOI   ScienceOn
12 Lee, K.-W., Choi, G.J., Kim, K.-Y., Ji, H.C., Park, H.S., Yoon, S.H., Lee, S.-H., 2009b. High frequency plant regeneration from mature seed derived callus of Italian ryegrass (Lolium multiflorum) cultivars. African Journal of Biotechnology. 8(24), 6828-6833.
13 Li, L., Li, R., Fei, S., Qu, R., 2005. Agrobacterium-mediated transformation of common bermudagrass (Cynodon dactylon) Plant Cell, Tissue and Organ Culture. 83, 223-229.   DOI   ScienceOn
14 Gugsa, L., Sarial, K., Lorz, H., Kumlehn, J., 2006. Gynogenic plant regeneration from unpollinated flower explants of teff [Eragrostis tef (Zuccagni) Trotter]. Journal of Plant Cell Reports. 25, 1287-1293.   DOI