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http://dx.doi.org/10.5851/kosfa.2022.e61

Optimal Pre-Plating Method of Chicken Satellite Cells for Cultured Meat Production  

Kim, So-Hee (Division of Applied Life Science (BK21 Four), Gyeongsang National University)
Kim, Chan-Jin (Division of Applied Life Science (BK21 Four), Gyeongsang National University)
Lee, Eun-Yeong (Division of Applied Life Science (BK21 Four), Gyeongsang National University)
Son, Yu-Min (Division of Applied Life Science (BK21 Four), Gyeongsang National University)
Hwang, Young-Hwa (Institute of Agriculture & Life Science, Gyeongsang National University)
Joo, Seon-Tea (Division of Applied Life Science (BK21 Four), Gyeongsang National University)
Publication Information
Food Science of Animal Resources / v.42, no.6, 2022 , pp. 942-952 More about this Journal
Abstract
To establish a pre-plating method of chicken satellite cells with high purity, pre-plating was performed under culture conditions of 37℃ and 41℃, and the pre-plating time was set from a total of 3 hours to 6 hours in consideration of the cell attachment time. The purity of the cells was confirmed by staining paired box protein 7 (Pax7) after proliferation, and Pax7 expression was the highest in culture flasks shaken for 2 hours after incubation at 41℃ for 2 hours to prevent the attachment of satellite cells (p<0.05). Also, when pre-plating and proliferation were performed at 37℃ and 41℃, the Pax7 expression rate was higher at 41℃. The differentiation capabilities of the three groups (T3, T6, and T7) with high Pax7 expression were compared and the fusion index (%) and myotube formation area (%) determined by myosin heavy chain (MHC) staining was calculated. The T6 and T7 groups, which were cultured at 41℃, showed significantly higher values than the T3 group (p<0.05). There was no significant difference in the expression of Pax7 and MHC between the T6 and T7 groups (p>0.05). These results suggest that pre-plating at 41℃ for a total of 4 hours was the most efficient in terms of cost and time for purifying chicken satellite cells for cultured meat.
Keywords
pre-plating; chicken satellite cell; purification; temperature; pre-plating time;
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