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http://dx.doi.org/10.5851/kosfa.2019.e25

Use of Fish Oil Nanoencapsulated with Gum Arabic Carrier in Low Fat Probiotic Fermented Milk  

Moghadam, Farideh Vahid (Department of Food Science and Technology, Sabzevar Branch, Islamic Azad University)
Pourahmad, Rezvan (Department of Food Science and Technology, College of Agriculture, VaraminPishva Branch, Islamic Azad University)
Mortazavi, Ali (Department of Food Science and Technology, Sabzevar Branch, Islamic Azad University)
Davoodi, Daryoush (Nanotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO))
Azizinezhad, Reza (Department of Biotechnology and Plant Breeding, College of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University)
Publication Information
Food Science of Animal Resources / v.39, no.2, 2019 , pp. 309-323 More about this Journal
Abstract
Fish oil consists of omega-3 fatty acids which play an important role in human health. Its susceptibility to oxidation causes considerable degradation during the processing and storage of food products. Accordingly, encapsulation of this ingredient through freeze drying was studied with the aim of protecting it against environmental conditions. Gum arabic (GA) was used as the wall material for fish oil nanoencapsulation where tween 80 was applied as the emulsifier. A water-in-oil (W/O) emulsion was prepared by sonication, containing 6% fish oil dispersed in aqueous solutions including 20% and 25% total wall material. The emulsion was sonicated at 24 kHz for 120 s. The emulsion was then freeze-dried and the nanocapsules were incorporated into probiotic fermented milk, with the effects of nanocapsules examined on the milk. The results showed that the nanoparticles encapsulated with 25% gum arabic and 4% emulsifier had the highest encapsulation efficiency (EE) (87.17%) and the lowest surface oil (31.66 mg/100 kg). Using nanoencapsulated fish oil in fermented milk significantly (p<0.05) increased the viability of Lactobacillus plantarum as well as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents. The fermented milk sample containing fish oil nanoencapsulated with 25% wall material and 4% emulsifier yielded the greatest probiotic bacterial count (8.41 Log CFU/mL) and the lowest peroxide value (0.57 mEq/kg). Moreover, this sample had the highest EPA and DHA contents. Utilizing this nanoencapsulated fish oil did not adversely affect fermented milk overall acceptance. Therefore, it can be used for fortification of low fat probiotic fermented milk.
Keywords
fermented milk; fish oil; nanoencapsulation; probiotic;
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1 Ackman RG. 2005. Fish oils in Baileys industrial oil and fat products. Jobz Wiley and Sons, Inc., Publication Hoboken, New Jersey, USA. pp 279-284.
2 AOCS. 2007. Offical methods and recommended practices of the American Oil Chemists Society. 7th ed. American Oil Chemists' Society, Champaign, Urbana, IL, USA.
3 Badee AZM, Amal E, El- Kader A, Hanan, MA. 2012. Microencapsulation of peppermint oil by spray drying. Aust J Basic Appl Sci 6:499-504.
4 Bibi S, Kaur R, Henriksen-Laceya M, McNeil SE, Wilkhu J, Lattmann E, Christensen D, Mohammed AR, Perrie Y. 2011. Microscopy imaging of liposomes: From coverslips to environmental SEM. Int J Pharm 417:138-150.   DOI
5 Borneo R, Kocer D, Ghai G, Tepper BJ, Karwe MV. 2007. Stability and consumer acceptance of long-chain omega-3 fatty acids (eicosapentaenoic acid, 20:5, n-3 and docosahexaenoic acid, 22:6, n-3) in cream-filled sandwich cookies. J Food Sci 72:S049-S054.   DOI
6 Chee P, Gallaher JJ, Djordjevic D, Faraji H, McClements DJ, Decker EA, Hollender R, Peterson DG, Roberts RF, Coupland JN. 2005. Chemical and sensory analysis of strawberry flavoured yogurt supplemented with an algae oil emulsion. J Dairy Res 72:311-316.   DOI
7 Damin MR, Minowa E, Alcantara MR, Oliveria MN. 2008. Effect of cold storage on culture viability and some rheological properties of fermented milk prepared with yogurt and probiotic bacteria. J Texture Stud 39:40-55.   DOI
8 Dave RI, Shah NP. 1997. Viability of yoghurt and probiotic bacteria in yoghurts made from commercial starter cultures. Int Dairy J 7:31-41.   DOI
9 Davidov-Pardo G, Roccia P, Salgado D, Leon AE, Pedroza-Islas R. 2008. Utilization of different wall materials to microencapsulate fish oil evaluation of its behavior in bread products. Am J Food Technol 3:384-393.   DOI
10 Shiga H, Yoshii H, Nishiyama T, Furuta T, Forssele P, Poutanen K. Linko P. 2001. Flavor encapsulation and release characteristics of spray-dried powder by the blended encapsulant of cyclodextrin and gum arabic. Dry Technol 19:1385-1395.   DOI
11 Smet K, Raes K, Block JD, Herman L, Dewettinck K, Coudijzer K. 2008. A change in antioxidative capacity as a measure of onset to oxidation in pasteurized milk. Int Dairy J 18:520-530.   DOI
12 Tamjidi F, Nasirpour A, Shahedi M. 2012. Physicochemical and sensory properties of yogurt enriched with microencapsulated fish oil. Food Sci Technol Int 18:381-390.   DOI
13 Vahid Moghadam F, Pourahmad R, Mortazavi A, Davoodi D, Azizinezhad R. (2019) Characteristics of freeze-dried nanoencapsulated fish oil with whey protein concentrate and gum arabic as wall materials. Food Sci Technol (Campinas) (in press). https://doi.org/10.1590/fst.22618.   DOI
14 Georgiera R, Iliev I, Haertle T, Chobert JM, Ivanova I, Danova S. 2009. Technological properties of candidate probiotic Lactobacillus plantarum strains. Int Dairy J 19:698-702.
15 Wang T, Deng Y, Geng Y, Gao Z, Zou J, Wang Z. 2006. Preparation of submicron unilamellar liposomes by freeze-drying double emulsions. Biochim Biophys Acta Biomembr 1758:222-231.   DOI
16 Ye A, Flanagan J, Singh H. 2006. Formation of stable nanoparticles via electrostatic complexation between sodium caseinate and gum arabic. Biopolymers 82:121-133.   DOI
17 Zimet P, Livney YD. 2009. Beta-lactoglobulin and its nanocamplexes with pectin as vehicles for $\omega$-3 polyunsaturated fatty acids. Food Hydrocoll 23:1120-1126.   DOI
18 Delavari M, Pourahmad R, Sokutifar R. 2014. Production of low fat synbiotic yogurt containing Lactobacillus plantarum and inulin. Adv Environ Biol 8:17-24.
19 Donkor ON, Nilmini SLI, Stolic P, Vasiljevic T, Shah NP. 2007. Survival and activity of selected probiotic organisms in settype yoghurt during cold storage. Int Dairy J 17:657-665.   DOI
20 Essid I, Medini M, Hassouna M. 2009. Technological and safety properties of Lactobacillus plantarum strains isolated from a Tunisian traditional salted meat. Meat Sci 81:203-208.   DOI
21 Ghayempour S, Mortazavi SM. 2015. Preparation and investigation of sodium alginate nanocapsules by different microemulsification devices. J Appl Polym Sci 132:344-349.
22 Ghorbanzade T, Jafari SM, Akhavan S, Hadavi R. 2017. Nano-encapsulation of fish oil in nano-liposomes and its application in fortification of yogurt. Food Chem 216:146-152.   DOI
23 Golestani M, Pourahmad R. 2017. Comparison of three treatments (two fermented treatments and one nonfermented treatment) in production of synbiotic ice cream. J Food Process Preserv 41:e12839.   DOI
24 Ilyasoglu H, Nehir El S. 2014. Nanoencapsulation of EPA/DHA with sodium caseinate-gum arabic complex and its usage in the enrichment of fruit juice. LWT-Food Sci Technol 56:461-468.   DOI
25 Izquierdo P, Esquena J, Tadros TF, Dederen C, Garcia MJ, Azemar N, Solans C. 2002. Formation and stability of nanoemulsions prepared using the phase inversion temperature method. Langmuir 18:26-30.   DOI
26 Jafari SM, Assadpoor E, Bhandari B, He Y. 2008. Nano-particle encapsulation of fish oil by spray drying. Food Res Int 41:172-183.   DOI
27 Klaypradit W, Huang YW. 2008. Fish oil encapsulation with chitosan using ultrasonic atomizer. LWT-Food Sci Technol 41:1133-1139.   DOI
28 Jimenez M, Garcia HS, Beristain CI. 2004. Spray-drying microencapsulation and oxidative stability of conjugated linoleic acid. Eur Food Res Technol 219:588-592.   DOI
29 Joung JY, Lee JY, Ha YS, Shin YK, Kim Y, Kim SH, Oh NS. 2016. Enhanced microbial, functional and sensory properties of herbal yogurt fermented with Korean traditional plant extracts. Korean J Food Sci Anim Resour 36:90-99.   DOI
30 Kaushik V, Ross YH. 2007. Limonene encapsulation in freeze-drying of gum arabic-sucrose-gelatin system. LWT-Food Sci Technol 40:1381-1391.   DOI
31 Klinkesorn U, Sophanodora P, Chinachoti P, Decker EA, McClements DJ. 2005. Encapsulation of emulsified tuna oil in twolayered interfacial membranes prepared using electrostatic layer-by-layer deposition. Food Hydrocoll 19:1044-1053.   DOI
32 Klinkesorn U, Sophanodora P, Chinachoti P, Decker EA, McClements DJ. 2006. Characterization of spray-dried tuna oil emulsified in two-layered interfacial membranes prepared using electrostatic layer-by-layer deposition. Food Res Int 39:449-457.   DOI
33 Krishnan S, Bhosale RG, Singhal RS. 2005. Microencapsulation of cardamom oleoresin: Evaluation of blends of gum arabic, maltodextrin and a modified starch as wall materials. Carbohyd Polym 61:95-102.   DOI
34 Mutka JR, Nelson DB. (1988). Preparation of encapsulated flavors with high flavor level. Food Technol 42:154-157.
35 Lavie CJ, Milani RV, Mehra MR, Ventura HO. 2009. Omega-3 polyunsaturated fatty acids and cardiovascular diseases. J Am Coll Cardiol 54:585-594.   DOI
36 Lee SJ, Hwang JH, Lee S, Ahn J, Kwak HS. 2007. Property changes and cholesterol-lowering effects in evening primrose oil-enriched and cholesterol-reduced yogurt. Int J Dairy Technol 60:22-30.   DOI
37 Lopez-Huertas E. 2010. Health effects of oleic acid and long chain omega-3 fatty acids (EPA and DHA) enriched milks. A review of intervention studies. Pharmacol Res 61:200-207.   DOI
38 Makri EA, Doxastakis GI. 2006. Study of emulsions and foams stabilized with Phaseolus vulgaris or Phaseolus coccineus with the addition of xanthan gum or NaCl. J Sci Food Agric 86:1863-1870.   DOI
39 McName BF, O'Riodan ED, O'Sullivan M. 1998. Emulsification and microencapsulation properties of gum Arabic. J Agric Food Chem 46:4551-4555.   DOI
40 Najaf Najafi M, Kadkhodaee R, Mortazavi SA. 2011. Effect of drying process and wall material on the properties of encapsulated cardamom oil. Food Biophys 6:68-76.   DOI
41 Rasti B, Erfanian A, Selamat J. 2017. Novel nanoliposomal encapsulated omega-3 fatty acids and their applications in food. Food Chem 230:690-696.   DOI
42 Saad N, Delattre C, Urdaci M, Schmitter JM, Bressollier P. 2013. An overview of the last advances in probiotic and prebiotic field. LWT-Food Sci Technol 55:1-16.   DOI
43 Sendra E, Fayos P, Lario Y, Fernandez-Lopez J, Sayas-Barbera E, Perez-Alvarez JA. 2008. Incorporation of citrus fibers in fermented milk containing probiotic bacteria. Food Microbiol 25:13-21.   DOI
44 Liu Z, Jiao Y, Wang Y, Zhou C, Zhang Z. 2008. Polysaccharides-based nanoparticles as drug delivery systems. Adv Drug Deliver Rev 60:1650-1662.   DOI
45 Saberi M, Hashemiravan M, Farhadyar N. 2014. Influence of casein and inulin on the properties of nano-particle encapsulation of fish oil. J Bio & Env Sci 4:318-326.