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http://dx.doi.org/10.5851/kosfa.2019.e16

Dynamics of Bacterial Communities of Lamb Meat Packaged in Air and Vacuum Pouch during Chilled Storage  

Wang, Taojun (Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University)
Guo, Huiyuan (Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University)
Zhang, Hao (Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University)
Ren, Fazheng (Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University)
Zhang, Ming (School of Food and Chemical Engineering, Beijing Technology and Business University)
Ge, Shaoyang (Beijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University)
Luo, Hailing (State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University)
Zhao, Liang (Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University)
Publication Information
Food Science of Animal Resources / v.39, no.2, 2019 , pp. 209-221 More about this Journal
Abstract
In this study, the changes in microbial communities of lamb meat packaged in the air (plastic tray, PT) and in a vacuum pouch (VAC) were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) during the storage at $4^{\circ}C$. For the PT lamb, the total viable count (TVC) was $10^7CFU/g$ on Day 5, and the dominated bacteria were Pseudomonas fragi, P. fluorescens, and Acinetobacter spp. For the VAC lamb, the TVC was $10^7CFU/g$ on Day 9, and the dominated bacteria were lactic acid bacteria, including Carnobacterium divergens, C. maltaromaticum, and Lactococcus piscium. One strain of Pseudomonas spp. also appeared in VAC lamb. The relative abundance of Enterobacteriaceae in VAC lamb was higher than that PT lamb, indicating a more important role of Enterobacteriaceae in spoilage for VAC lamb than that of PT lamb. The microbial compositions changed faster in the lamb stored in a PT than that stored in a VAC, and microbial community compositions of the late storage period were largely different from those of the early storage period for both the conditions. The findings of this study may guide improve the lamb hygiene and prolong the shelf life of the lamb.
Keywords
chilled lamb; bacterial community; storage; PCR-DGGE;
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1 Ercolini D, Russo F, Torrieri E, Masi P, Villani F. 2006. Changes in the spoilage-related microbiota of beef during refrigerated storage under different packaging conditions. Appl Environ Microbiol 72:4663-4671.   DOI
2 Gill CO, Newton KG. 1979. Spoilage of vacuum-packaged dark, firm, dry meat at chill temperatures. Appl Environ Microbiol 37:362-364.   DOI
3 Gram L, Ravn L, Rasch M, Bruhn JB, Christensen AB, Givskov M. 2002. Food spoilage - interactions between food spoilage bacteria. Int J Food Microbiol 78:79-97.   DOI
4 Hauge SJ, Nafstad O, Skjerve E, Rotterud OJ, Nesbakken T. 2011. Effects of shearing and fleece cleanliness on microbiological contamination of lamb carcasses. Int J food microbiol 150:178-183.   DOI
5 Jiang Y, Gao F, Xu XL, Su Y, Ye KP, Zhou GH. 2010. Changes in the bacterial communities of vacuum-packaged pork during chilled storage analyzed by pcr-dgge. Meat Sci 86:889-895.   DOI
6 Kiermeier A, Tamplin M, May D, Holds G, Williams M, Dann A. 2013. Microbial growth, communities and sensory characteristics of vacuum and modified atmosphere packaged lamb shoulders. Food Microbiol 36:305-315.   DOI
7 Kim C, Stein RA, Pao S. 2015. Comparison of the microbial quality of lamb and goat meat acquired from internet and local retail markets. J Food Prot 78:1980-1987.   DOI
8 Lubbs DC, Vester BM, Fastinger ND, Swanson KS. 2009. Dietary protein concentration affects intestinal microbiota of adult cats: A study using DGGE and qPCR to evaluate differences in microbial populations in the feline gastrointestinal tract. J Anim Physiol Anim Nutr 93:113-121.   DOI
9 Ercolini D, Ferrocino I, Nasi A, Ndagijimana M, Vernocchi P, La Storia A, Laghi L, Mauriello G, Guerzoni ME, Villani F. 2011. Monitoring of microbial metabolites and bacterial diversity in beef stored under different packaging conditions. Appl Environ Microbiol 77:7372-7381.   DOI
10 Meng Y, Zhang J. 2018. Beef and mutton markets in 2017 and their outlooks for 2018. Agric Outlook 14:9-12.
11 Mills J, Donnison A, Brightwell G. 2014. Factors affecting microbial spoilage and shelf-life of chilled vacuum-packed lamb transported to distant markets: A review. Meat Sci 98:71-80.   DOI
12 Oses SM, Diez AM, Melero B, Luning PA, Jaime I, Rovira J. 2013. Characterization by culture-dependent and cultureindependent methods of the bacterial population of suckling-lamb packaged in different atmospheres. Food Microbiol 36:216-222.   DOI
13 Muyzer G, De waal EC, Uitterlinden AG. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16s rrna. Appl Environ Microbiol 59:695-700.   DOI
14 Newton KG, Harrison JCL, Smith KM. 1977. The effect of storage in various gaseous atmospheres on the microflora of lamb chops held at $-1^{\circ}C$. J Appl Bacteriol 43:53-59.   DOI
15 Ortuno J, Serrano R, Banon S. 2017. Incorporating rosemary diterpenes in lamb diet to improve microbial quality of meat packed in different environments. Anim Sci J 88:1436-1445.   DOI
16 Pennacchia C, Ercolini D, Villani F. 2011. Spoilage-related microbiota associated with chilled beef stored in air or vacuum pack. Food Microbiol 28:84-93.   DOI
17 Sani MA, Ehsani A, Hashemi M. 2017. Whey protein isolate/cellulose nanofibre/tio2 nanoparticle/rosemary essential oil nanocomposite film: Its effect on microbial and sensory quality of lamb meat and growth of common foodborne pathogenic bacteria during refrigeration. Int J Food Microbiol 251:8-14.   DOI
18 Schobert M, Jahn D. 2010. Anaerobic physiology of Pseudomonas aeruginosa in the cystic fibrosis lung. Int J Med Microbiol 300:549-556.   DOI
19 Stoops J, Ruyters S, Busschaert P, Spaepen R, Verreth C, Claes J, Lievens B, Van Campenhout L. 2015. Bacterial community dynamics during cold storage of minced meat packaged under modified atmosphere and supplemented with different preservatives. Food Microbiol 48:192-199.   DOI
20 Soldatou N, Nerantzaki A, Kontominas MG, Savvaidis IN. 2009. Physicochemical and microbiological changes of "souvlaki" - a greek delicacy lamb meat product: Evaluation of shelf-life using microbial, colour and lipid oxidation parameters. Food Chem 113:36-42.   DOI
21 Sun XD, Holley RA. 2012. Antimicrobial and antioxidative strategies to reduce pathogens and extend the shelf life of fresh red meats. Compr Rev Food Sci Food Saf 11:340-354.   DOI
22 Via LE, Falkinham JO. 1995. Comparison of methods for isolation of Mycobacterium avium complex DNA for use in pcr and rapd fingerprinting. J Microbiol Methods 21:151-161.   DOI
23 Wang T, Zhao L, Sun Y, Ren F, Chen S, Zhang H, Guo H. 2016. Changes in the microbiota of lamb packaged in a vacuum and in modified atmospheres during chilled storage analysed by high-throughput sequencing. Meat Sci 121:253-260.   DOI
24 Zhang HY, Yang B, Luo RM, Wu LL, Su CX. 2016. Analysis of bacterial community diversity in cold fresh tan lamp during different storage time using pyrosequencing. Sci Technol Food Ind 37:177-182.
25 Zhao F, Zhou G, Ye K, Wang S, Xu X, Li C. 2015. Microbial changes in vacuum-packed chilled pork during storage. Meat Sci 100:145-149.   DOI
26 Zhou Y, Ai Q, Zhang D. 2015. Changes in microflora on fresh mutton during chilled storage. Food Sci 36:242-245.
27 Casaburi A, Piombino P, Nychas GJ, Villani F, Ercolini D. 2015. Bacterial populations and the volatilome associated to meat spoilage. Food Microbiol 45:83-102.   DOI
28 Balamatsia CC, Patsias A, Kontominas MG, Savvaidis IN. 2007. Possible role of volatile amines as quality-indicating metabolites in modified atmosphere-packaged chicken fillets: Correlation with microbiological and sensory attributes. Food Chem 104:1622-1628.   DOI
29 Biss ME, Hathaway SC. 1996. Effect of pre-slaughter washing of lambs on the microbiological and visible contamination of the carcases. Vet Rec 138:82-86.   DOI
30 Brightwell G, Clemens R, Urlich S, Boerema J. 2007. Possible involvement of psychrotolerant enterobacteriaceae in blown pack spoilage of vacuum-packaged raw meats. Int J Food Microbio 119:334-339.   DOI
31 Doulgeraki AI, Ercolini D, Villani F, Nychas GJE. 2012. Spoilage microbiota associated to the storage of raw meat in different conditions. Int J Food Microbiol 157:130-141.   DOI
32 Cleveland J, Montville TJ, Nes IF, Chikindas ML. 2001. Bacteriocins: Safe, natural antimicrobials for food preservation. Int J Food Microbiol 71:1-20.   DOI