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http://dx.doi.org/10.5851/kosfa.2016.36.6.752

Mathematical Model for Predicting the Growth Probability of Staphylococcus aureus in Combinations of NaCl and NaNO2 under Aerobic or Evacuated Storage Conditions  

Lee, Jeeyeon (Department of Food and Nutrition, Sookmyung Women's University)
Gwak, Eunji (Department of Food and Nutrition, Sookmyung Women's University)
Ha, Jimyeong (Department of Food and Nutrition, Sookmyung Women's University)
Kim, Sejeong (Department of Food and Nutrition, Sookmyung Women's University)
Lee, Soomin (Department of Food and Nutrition, Sookmyung Women's University)
Lee, Heeyoung (Department of Food and Nutrition, Sookmyung Women's University)
Oh, Mi-Hwa (National Institute of Animal Science, RDA)
Park, Beom-Young (National Institute of Animal Science, RDA)
Oh, Nam Su (R&D Center, Seoul Dairy Cooperative)
Choi, Kyoung-Hee (Department of Oral Microbiology, College of Dentistry, Wonkwang University)
Yoon, Yohan (Department of Food and Nutrition, Sookmyung Women's University)
Publication Information
Food Science of Animal Resources / v.36, no.6, 2016 , pp. 752-759 More about this Journal
Abstract
The objective of this study was to describe the growth patterns of Staphylococcus aureus in combinations of NaCl and $NaNO_2$, using a probabilistic model. A mixture of S. aureus strains (NCCP10826, ATCC13565, ATCC14458, ATCC23235, and ATCC27664) was inoculated into nutrient broth plus NaCl (0, 0.25, 0.5, 0.75, 1, 1.25, 1.5, and 1.75%) and $NaNO_2$ (0, 15, 30, 45, 60, 75, 90, 105, and 120 ppm). The samples were then incubated at 4, 7, 10, 12 and $15^{\circ}C$ for up to 60 d under aerobic or vacuum conditions. Growth responses [growth (1) or no growth (0)] were then determined every 24 h by turbidity, and analyzed to select significant parameters (p<0.05) by a stepwise selection method, resulting in a probabilistic model. The developed models were then validated with observed growth responses. S. aureus growth was observed only under aerobic storage at $10-15^{\circ}C$. At $10-15^{\circ}C$, NaCl and $NaNO_2$ did not inhibit S. aureus growth at less than 1.25% NaCl. Concentration dependency was observed for NaCl at more than 1.25%, but not for $NaNO_2$. The concordance percentage between observed and predicted growth data was approximately 93.86%. This result indicates that S. aureus growth can be inhibited in vacuum packaging and even aerobic storage below $10^{\circ}C$. Furthermore, $NaNO_2$ does not effectively inhibit S. aureus growth.
Keywords
predictive model; S. aureus; NaCl; $NaNO_2$; processed meat products;
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