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http://dx.doi.org/10.5851/kosfa.2014.34.6.815

Changes in the Microbiological Characteristics of Korean Native Cattle (Hanwoo) Beef Exposed to Ultraviolet (UV) Irradiation Prior to Refrigeration  

Kim, Hyun-Jung (Department of Food Science and Technology and Functional Food Research Center, Chonnam National University)
Lee, Yong-Jae (Food Protein R&D Center, Texas A&M University)
Eun, Jong-Bang (Department of Food Science and Technology and Functional Food Research Center, Chonnam National University)
Publication Information
Food Science of Animal Resources / v.34, no.6, 2014 , pp. 815-821 More about this Journal
Abstract
The effects of ultraviolet (UV) radiation were investigated with regards to the microbial growth inhibitory effect on the shelf life of Korean native cattle (Hanwoo) beef prior to refrigerated storage. The Hanwoo samples were exposed to UV radiation ($4.5mW/cm^2$) for 0, 5, 10, 15, and 20 min. The UV-irradiated beef that was exposed for 20 min showed significantly reduced mesophilic and psychrotrophic bacterial populations to the extent of approximately 3 log cycles, as compared to that of non-irradiated beef. About 2.5 Log CFU/g of mesophilic bacteria were different compared with UV-irradiated and non-irradiated meat. UV irradiation showed the most significant growth inhibition effects on mesophilic and psychrotrophic bacteria. Coliform and Gram-negative bacteria were also reduced by 1 log cycle. The population of L. monocytogenes, S. Typhimurium, and E. coli O157:H7 decreased significantly to 53.33, 39.68, and 45.76% after 10 min of UV irradiation. They decreased significantly to 84.64, 80.76, and 84.12%, respectively, after 20 min of UV irradiation. The results show that UV irradiation time and the inhibitory effect were proportional. These results verified that UV radiation prior to refrigeration can effectively reduce the number of pathogenic bacteria on the surface of meat and improve the meat's microbial safety.
Keywords
ultraviolet (UV) irradiation; Korean native cattle (Hanwoo) beef; pathogenic bacteria;
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1 Allende, A. and Artes, F. (2003) Combined ultraviolet-C and modified atmosphere packaging treatments for reducing microbial growth of fresh processed lettuce. LWT-Food Sci. Technol. 36, 779-786.   DOI   ScienceOn
2 Wilson, C. L., Ghaouth, A. E., Upchurch, B., Stevens, C., Khan, V., Droby, S., and Chalutz, E. (1997) Using an on-line UV-C apparatus to treat harvested fruit for controlling postharvest decay. Hort. Technol. 7, 278-281.
3 Winger, R. J. and Fennema, O. (1976) Tenderness and water holding properties of Beef muscle as influenced by freezing and subsequent storage at -3 or $15^{\circ}C$. J. Food Sci. 41, 1433-1438.   DOI
4 Wong, E., Linton, R. H., and Gerrard, D. E. (1998) Reduction of Escherichia coli and Salmonella senftenberg on pork skin and pork muscle using ultraviolet light. Food Microbiol. 15, 415-423.   DOI   ScienceOn
5 Stevens, C., Khan, V. A., Tang, A. Y., and Lu, J. Y. (1990) The effect of ultraviolet radiation on mold rots and nutrients of stored sweet potatoes. J. Food Prot. 3, 194-274.
6 Samelis, J. (2006) Managing microbial spoilage in the meat industry. In: Blackburn, C. (Ed.), Food Spoilage Microorganisms. Woodhead Publishing, Cambridge, pp. 213-286.
7 Sastry, S. K., Datta, A. K., and Worobo, R. W. (2000) Ultraviolet light. J. Food Sci. Suppl. 65, 90-92.   DOI
8 Sumner, S. S., Wallner-Pendleton, E. A., Froning, G. W., and Stetson, L. E. (1996) Inhibition of Salmonella tyhimurium on agar medium and poultry skin by ultraviolet energy. J. Food Prot. 59, 319-321.   DOI
9 Sofos, J. N. (2008) Challenges to meat safety in the 21st century. Meat Sci. 78, 3-13.   DOI   ScienceOn
10 Sucre, E., Vidussi, F., Mostajir, B., Charmantier, G., and Lorin- Nebel, C. (2012) Impact of ultraviolet-B radiation on planktonic fish larvae: Alteration of the osmoregulatory function. Aquat. Toxicol. 109, 194-201.   DOI   ScienceOn
11 Thayer, D. W. (1994) Wholesomeness of irradiated foods. Food Technol. 48, 58-67.
12 Unda, J. R., Molins, R. A., and Walker, H. W. (1990) Microbiological and some physical and chemical changes in vacuum- packaged beef steaks treat with combinations of potassium sorbate, phosphate, sodium chloride and sodium acetate. J. Food Sci. 55, 323-326.   DOI
13 Unluturk, S., Atilgan, M. R., Baysal, A. H., and Tari, C. (2008) Use of UV-C radiation as a non-thermal process for liquid egg products (LEP). J. Food Eng. 85, 561-568.   DOI   ScienceOn
14 Zhou, G. H., Xy, X. L., and Liu, Y. (2010) Preservation technologies for fresh meat-A review. Meat Sci. 86, 119-128.   DOI   ScienceOn
15 Kang, C. G., Ko J. S., Kwon I. k., Kim, G. Y., Park, G. B., Park, S. Y., Pak, J. I., Lee, S. K., Chae, Y. S., Choi, M., and Choe I. S. (1997) Science of animal products. Storage and putrefaction of meat, Yu Han Mun Hwa Sa, Seoul, Korea, p. 293.
16 Kim, D. G., Lee, S. M., Kim, S. M., Seok, Y. S., and Sung, S. K. (1992) Effects of packaging method on physico-chemical properties of Korean native beef. J. Korean Sci. Nutr. 25, 944-950.
17 Park, S. Y., Lee, N. Y., Kim, S. H., Cho, J. I., Lee, H. J., and Ha, S. D. (2014) Effect of ultraviolet radiation on the reduction of major food spoilage molds and sensory quality of the surface of dried filefish (Stephanolepis cirrhifer) fillets. Food Res. Int. 62, 1108-1112.   DOI   ScienceOn
18 Lu, J. Y., Stevens, C., Yakubu, P., and Loretan, P. A. (1987) Gamma, lectron beam and ultraviolet radiation on control of storage rots and quality of Walla Walla onions. J. Food Process. Pres. 12, 53-62.
19 Lyon, B. A., Dotson, A. D., Linden, K. G., and Weinberg, H. S. (2012) The effect of inorganic precursors on disinfection byproduct formation during UV-chlorine/chloramine drinking water treatment. Water Res. 46, 4653-4664.   DOI   ScienceOn
20 Park, J. S. and Hwang, H. S. (1996) SAS Programming introduction. Kyung Moon Sa, Seoul, Korea, p. 207.
21 Perez-Gregorio, M. R., Gonzalez-Barreiro, C., Rial-Otero, R., and Simal-Gandara, J. (2011) Comparison of Sanitizing technologies on the quality appearance and antioxidant levels in onion slices. Food Control 22, 2052-2058.   DOI   ScienceOn
22 Podolak, R. K., Zayas, J. F., Kastner, C. L., and Fung, D. Y. C. (1996) Inhibition of Listeria monocytogenes and Escherichia coli O157:H7 on beef by application of organic acids. J. Food Prot. 59, 370-373.   DOI
23 Ryeong, M. S. I. (1996) The UVO-type Humidification Cooling Sterilizer. Food Ind. 73-78.
24 Dogbevi, M. K., Vachon, C., and Lacroix, L. (1999) Physic ochemical and microbiological changes in irradiated fresh pork loins. Meat Sci. 51, 349-354.   DOI   ScienceOn
25 Firstenberg-Eden, R. (1981) Attachment of bacteria to meat surfaces: a review. J. Food Prot. 44, 602-607.   DOI
26 Guan, W., Fan, X., and Yan, R. (2012) Effects of UV-C treatment on inactivation of Escherichia coli O157:H7, microbial loads, and quality of button mushrooms. Postharvest Biol. Tec. 64, 119-125.   DOI   ScienceOn
27 Ehiba, R. M., Kraft, A. A., Molins, R. A., Walker, H. W., Olson, D. G., Subbaraman, G., and Skowronski, R. P. (1987) Effect of low-dose (100 krad) gamma radiation on the microflora of vacuum-packaged ground pork with and without added sodium phosphates. J. Food Sci. 52, 1477-1480.   DOI
28 Ercolini, D., Russo, F., Nasi, A., Ferranti, P., and Villani, F. (2009) Mesophilic and pshychrotrophic bacteria from meat and their spoilage potential in vitro and in beef. Appl. Environ. Microbiol. 75, 1990-2001.   DOI   ScienceOn
29 Harrington, W. O. and Claude, H. H. (1968) Reduction of the microbial population apple cider by ultraviolet irradiation. Food Technol.22, 1451-1454.
30 Huang, Y. W. and Toledo, R. (1982) Effect of high doses of high and low intensity UV irradiation on the growth of microbial counts and storage-life of fish. J. Food Sci. 47, 1667-1670.   DOI
31 Joo, H. K., Kim, D. W., Sung H. J., and Cho, W. D. (1998) The latest food preservation. Storage of various food. Soo Hak Sa, Seoul, Korea, p. 350.
32 Junillon, T. and Flandrois, J. (2014) Diminution of 2,3,5-triphenyltetrazolium chloride toxicity on Listeria monocytogenes growth by iron source addition to the culture medium. Food Microbiol. 38, 1-5.   DOI   ScienceOn
33 Barnes, E. M. (1976) Microbiological problems of poultry at refrigerator temperatures-A review. J. Sci. Food Agr. 27, 777-782.   DOI
34 Assatarakul, K., Churey, J. J., Manns, D. C., and Worobo, R. W. (2012) Patulin reduction in apple juice from concentrate by UV radiation and comparison of kinetic degradation models between apple juice and apple cider. J. Food Prot. 75, 717-724.   DOI   ScienceOn
35 Bahmani, Z. A., Rezai, M., Hosseini, S. V., Regenstein, J. M., Bohme, K., Alishahi, A., and Yadollahi, F. (2011) Chilled storage of golden gray mullet (Liaz aurata). LWT-Food Sci. Technol. 44, 1894-1900.   DOI   ScienceOn
36 Barbut, S. (2002) Poultry products - formations and gelation, in: Barbut, S. (Ed.). Poultry products processing: An Industry Guide, New Yor, NY, CRC Press.
37 Brewer, M. S., Lkins, W. G., and Harberts, C. A. Z. (1992) TBA values sensory characteristics and volatiles in ground pork during long-term frozen storage: Effect of packaging. J. Food Sci. 57, 558-580.   DOI
38 Chan, C. F., Lien, C. Y., Lai, Y. C., Huang, C. L., and Liao, W. C. (2010) Influence of purple sweet potato extracts on the UV absorption properties of a cosmetic cream. J. Cosmet. Sci. 61, 333-341.
39 Chun, H. H., Kim, J. Y., Lee, B. D., Yu, D. J., and Song, K. B. (2010) Effect of UV-C irradiation on the inactivation of inoculated pathogens and quality of chicken breasts during storage. Food Control 21, 276-280.   DOI   ScienceOn
40 Chung, K. T., Dickson, J. S., and Crouse, J. D. (1989) Attachment and proliferation of bacteria on meat. J. Food Prot. 52, 173-177.   DOI
41 Moseley, B. E. B. (1990) In D. E. Johnson, & M. H. Stevenson (Eds.), Food irradiation and the chemist. Bristol: Royal Society of Chemistry, 97-108.
42 Devine, D. A., Keech, A. P., Wood, D. J., Killington, R. A., Boyes, H., Doubleday, B., et al. (2001) Ultraviolet disinfection with a novel microwave-powered device. J. Appl. Microbiol. 91, 786-794.   DOI   ScienceOn
43 US Food and Drug Administration (2007) Irradiation in the production, processing and handling of food: 21CFR. Part 179.39. Code of Federal Regulations, 3, 439-440.
44 Costerson, J. W., Irvin, R. T., and Cheng, K. J. (1981) The bacterial glycocalyx in nature and disease. Annu. Rev. Microbiol. 35, 299-324.   DOI   ScienceOn