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http://dx.doi.org/10.5851/kosfa.2014.34.5.570

Comparison Study on Changes of Antigenicities of Egg Ovalbumin Irradiated by Electron Beam or X-Ray  

Kim, Mi-Jung (Department of Food Science and Nutrition, Anyang University)
Lee, Ju-Woon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Sung, Nak-Yoon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Kim, Su-Min (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Hwang, Young-Jung (Department of Food Service and Culinary, International University of Korea)
Kim, Jae-Hun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Song, Beom-Seok (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Publication Information
Food Science of Animal Resources / v.34, no.5, 2014 , pp. 570-575 More about this Journal
Abstract
This study was conducted to compare the effects of two forms of radiation (electron and X-ray; generated by an electron beam accelerator) on the conformation and antigenic properties of hen's egg albumin, ovalbumin (OVA), which was used as a model protein. OVA solutions (2.0 mg/mL) were individually irradiated by electron beam or X-ray at the absorbed doses of 0 (control), 2, 4, 6, 8, and 10 kGy. No differences between the two forms of radiation on the structural properties of OVA were shown by spectrometric and electrophoretic analyses. The turbidity of OVA solution increased and the main OVA bands on polyacrylamide gels disappeared after irradiation, regardless of the radiation source. In competitive indirect enzyme-linked immunosorbent assay, OVA samples irradiated by electron beam or X-ray showed different immunological responses in reactions with monoclonal and polyclonal antibodies (immunoglobulin G) produced against non-irradiated OVA. The results indicate that electron beam irradiation and X-ray irradiation produced different patterns of structural changes to the OVA molecule.
Keywords
electron beam; X-ray; ovalbumin; conformational change;
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