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http://dx.doi.org/10.5851/kosfa.2013.33.3.317

Inactivation of Campylobacter jejuni using Radio-frequency Atmospheric Pressure Plasma on Agar Plates and Chicken Hams  

Kim, Joo-Sung (Korea Food Research Institute)
Lee, Eun-Jung (Korea Food Research Institute)
Cho, Eun-Ah (Korea Food Research Institute)
Kim, Yun-Ji (Korea Food Research Institute)
Publication Information
Food Science of Animal Resources / v.33, no.3, 2013 , pp. 317-324 More about this Journal
Abstract
Radio-frequency driven atmospheric pressure plasma using argon gas was studied in the inactivation of Campylobacter jejuni in order to investigate its applicability. First, the inactivation study was conducted on an agar surface. C. jejuni NCTC11168 was reduced by more than 7 Log CFU after an 88 s treatment. Another strain, ATCC49943, was studied; however, the inactivation was less efficient, with a 5 Log CFU reduction after a 2 min treatment. Then, chicken breast ham was studied at the $10^6$ CFU inoculation level. The inactivation efficiency was much lower for both strains compared to that on the agar plates. C. jejuni NCTC11168 and ATCC49943 were reduced by 3 Log CFU after a 6 min treatment and by 1.5 Log CFU after a 10 min treatment, respectively. The scanning electron microscopy analysis indicated that C. jejuni cells were deformed or transformed into coccoid form under the plasma treatment. During the plasma treatment, the temperature of the samples did not rise above $43^{\circ}C$, suggesting that heat did not contribute to the inactivation. Meanwhile, water activity significantly decreased after a 10 min treatment (p<0.05). This study conveyed that radio-frequency atmospheric pressure plasma can effectively inactivate C. jejuni with strain-specific variation.
Keywords
Campylobacter jejuni; radio-frequency atmospheric pressure plasma; inactivation; chicken breast ham; scanning electron microscopy;
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1 Srivastava, N., and Wang, C. (2011) Effects of water addition on OH radical generation and plasma properties in an atmospheric argon microwave plasma jet. J. Appl. Phys. 110, 053304.   DOI   ScienceOn
2 Sureshkumar, A., Sankar, R., Mandal, M., and Neogi, S. (2010) Effective bacterial inactivation using low temperature radio frequency plasma. Int. J. Pharm. 396, 17-22.   DOI   ScienceOn
3 Tangwatcharin, P., Chanthachum, S., Khopaibool, P., and Griffiths, M. W. (2006) Morphological and physiological responses of Campylobacter jejuni to stress. J. Food Prot. 69, 2747-2753.
4 Tendero, C., Tixier, C., Tristant, P., Desmaison, J., and Leprince, P. (2006) Atmospheric pressure plasmas: A review. Spectrochimica Acta Part B. 61, 2-30.   DOI   ScienceOn
5 von Keudell, A., Awakowicz, P., Benedikt, J., Raballand, V., Yanguas-Gil, A., Opretzka, J., Flotgen, C., Reuter, R., Byelykh, L., Halfmann, H., Stapelmann, K., Denis, B., Wunderlich, J., Muranyi, P., Rossi, F., Kylian, O., Hasiwa, N., Ruiz, A., Rauscher, H., Sirghi, L., Comoy, E., Dehen, C., Challier, L., and Deslys, J. P. (2010) Inactivation of bacteria and biomolecules by low-pressure plasma discharges. Plasma Process. Polym. 7, 327-352.   DOI   ScienceOn
6 Wareing, D. R., Bolton, F. J., Fox, A. J., Wright, P. A., and Greenway, D. L. (2002) Phenotypic diversity of Campylobacter isolates from sporadic cases of human enteritis in the UK. J. Appl. Microbiol. 92, 502-509.   DOI   ScienceOn
7 Yang, B., Chen, J., Yu, Q., Li, H., Lin, M., Mustapha, A., Hong, L., and Wang, Y. (2011) Oral bacterial deactivation using a lowtemperature atmospheric argon plasma brush. J. Dent. 39, 48-56.   DOI   ScienceOn
8 Young, V. B. and Mansfield, L. S. (2005) Campylobacter infection- clinical context. In: Campylobacter molecular and cellular biology. Ketley, J. M. and Konkel, M. E. (ed) Horizon bioscience, UK, pp. 1-12.
9 Yun, H., Kim, B., Jung, S., Kruk, Z. A., Kim, D. B., Choe, W., and Jo, C. (2010) Inactivation of Listeria monocytogenes inoculated on disposable plastic tray, aluminum foil, and paper cup by atmospheric pressure plasma. Food Control 21, 1182-1186.   DOI   ScienceOn
10 Kim, B., Yun, H., Jung, S., Jung, Y., Jung, H., Choe, W., and Jo, C. (2011) Effect of atmospheric pressure plasma on inactivation of pathogens inoculated onto bacon using two different gas compositions. Food Microbiol. 28, 9-13.   DOI   ScienceOn
11 Korachi, M., Gurol, C., and Aslan, N. (2010) Atmospheric plasma discharge sterilization effects on whole cell fatty acid profiles of Escherichia coli and Staphylococcus aureus. J. Electrostatics 68, 508-512.   DOI   ScienceOn
12 Laroussi, M. (2005) Low temperature plasma-based sterilization: overview and state-of-the-art. Plasma Process Polym. 2, 391-400.   DOI   ScienceOn
13 Moran, A. P. and Upton, M. E. (1987a) Effect of medium supplements, illumination and superoxide dismutase on the production of coccoid forms of Campylobacter jejuni ATCC 29428. J. Appl. Bacteriol. 62, 43-51.   DOI   ScienceOn
14 Moran, A. P. and Upton, M. E. (1987b) Factors affecting production of coccoid forms by Campylobacter jejuni on solid media during incubation. J. Appl. Bacteriol. 62, 527-537.   DOI   ScienceOn
15 Moreau, M., Orange, N., and Feuilloley, M. G. (2008) Nonthermal plasma technologies: New tools for bio-decontamination. Biotechnol. Adv. 26, 610-617.   DOI   ScienceOn
16 Niemira, B. A. and Sites, J. (2008) Cold plasma inactivates Salmonella Stanley and Escherichia coli O157:H7 inoculated on golden delicious apples. J. Food Prot. 71, 1357-1365.
17 Park, S. F. (2002) The physiology of Campylobacter species and its relevance to their role as foodborne pathogens. Int. J. Food Microbiol. 74, 177-188.   DOI   ScienceOn
18 Perni, S., Shama, G., and Kong, M. G. (2008b) Cold atmospheric plasma disinfection of cut fruit surfaces contaminated with migrating microorganisms. J. Food Prot. 71, 1619-1625.
19 Parker, C. T., Quinones, B., Miller, W. G., Horn, S. T., and Mandrell, R. E. (2006) Comparative genomic analysis of Campylobacter jejuni strains reveals diversity due to genomic elements similar to those present in C. jejuni strain RM1221. J. Clin. Microbiol. 44, 4125-4135.   DOI   ScienceOn
20 Perni, S., Liu, D. W., Shama, G., and Kong, M. G. (2008a) Cold atmospheric plasma decontamination of the pericarps of fruit. J. Food Prot. 71, 302-308.
21 Rod, S. K., Hansen, F., Leipold, F., and Knochel, S. (2012) Cold atmospheric pressure plasma treatment of ready-to-eat meat: Inactivation of Listeria innocua and changes in product quality. Food Microbiol. 30, 233-238.   DOI   ScienceOn
22 Scallan, E., Hoekstra, R. M., Angulo, F. J., Tauxe, R. V., Widdowson, M. A., Roy, S. L., Jones, J. L., and Griffin, P. M. (2011) Foodborne illness acquired in the United States--major pathogens. Emerg. Infect. Dis. 17, 7-15.   DOI
23 Senorans, J., Ibanez, E., and Cifuentes, A. (2003) New trends in food processing. Crit. Rev. Food Sci. Nutr. 43, 507-526.   DOI   ScienceOn
24 Serra, X., Ruiz-Ramírez, J., Arnau, J., and Gou, P. (2005) Texture parameters of dry-cured ham m. biceps femoris samples dried at different levels as a function of water activity and water content. Meat Sci. 69, 249-254.   DOI   ScienceOn
25 AOAC. (2005) Official methods of analysis of the Association of Analytical Chemistry. Association of Official Analytical Chemists, Washington, DC, USA.
26 Buck, G. E., Parshall, K. A., and Davis, C. P. (1983) Electron microscopy of the coccoid form of Campylobacter jejuni. J. Clin. Microbiol. 18, 420-421.
27 Dorrell, N., Mangan, J. A., Laing, K. G., Hinds, J., Linton, D., Al-Ghusein, H., Barrell, B. G., Parkhill, J., Stoker, N. G., Karlyshev, A. V., Butcher, P. D., and Wren, B. W. (2001) Whole genome comparison of Campylobacter jejuni human isolates using a low-cost microarray reveals extensive genetic diversity. Genome Res. 11, 1706-1715.   DOI   ScienceOn
28 Critzer, F. J., Kelly-Wintenberg, K., South, S. L., and Golden, D. A. (2007) Atmospheric plasma inactivation of foodborne pathogens on fresh produce surfaces. J. Food Prot. 70, 2290-2296.
29 Deng, S., Cheng, C., Ni, G., Meng, Y., and Chen, H. (2010) Bacillus subtilis devitalization mechanism of atmosphere pressure plasma jet. Curr. Appl. Phys. 10, 1164-1168.   DOI   ScienceOn
30 Dirks, B. P., Dobrynin, D., Fridman, G., Mukhin, Y., Fridman, A., and Quinlan, J. J. (2012) Treatment of raw poultry with nonthermal dielectric barrier discharge plasma to reduce Campylobacter jejuni and Salmonella enterica. J. Food Prot. 75, 22-28.   DOI   ScienceOn
31 Fernandez, A., Noriega, E., and Thompson, A. (2013) Inactivation of Salmonella enterica serovar Typhimurium on fresh produce by cold atmospheric gas plasma technology. Food Microbiol. 33, 24-29.   DOI   ScienceOn
32 He, Y. and Chen, C. Y. (2010) Quantitative analysis of viable, stressed and dead cells of Campylobacter jejuni strain 81-176. Food Microbiol. 27, 439-446.   DOI   ScienceOn
33 Hofreuter, D., Novik, V., and Galan, J. E. (2008) Metabolic diversity in Campylobacter jejuni enhances specific tissue colonization. Cell Host Microbe. 4, 425-433.   DOI   ScienceOn
34 Hofreuter, D., Tsai, J., Watson, R. O., Novik, V., Altman, B., Benitez, M., Clark, C., Perbost, C., Jarvie, T., Du, L., and Galan, J. E. (2006) Unique features of a highly pathogenic Campylobacter jejuni strain. Infect. Immun. 74, 4694-4707.   DOI   ScienceOn
35 Humphrey, T., O'Brien, S., and Madsen, M. (2007) Campylobacters as zoonotic pathogens: a food production perspective. Int. J. Food Microbiol. 117, 237-257.   DOI   ScienceOn
36 Jin, S.-K., Shin, D., and Hur, I.-C. (2011) Effects of moisture content on quality characteristics of dry-cured ham during storage. Korean J. Food Sci. An. 31, 756-762.   과학기술학회마을   DOI   ScienceOn
37 Jang, K. I., Kim, M. G., Ha, S. D., Kim, K. S., Lee, K. H., Chung, D. H., Kim, C. H., and Kim, K. Y. (2007) Morphology and adhesion of Campylobacter jejuni to chicken skin under varying conditions. J. Microbiol. Biotechnol. 17, 202-206.