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Antimicrobial and Antitumoral Activities of Candida kefyr TEP 7 Isolated from Tibetan Fermented Milk  

윤원호 (서일대학 식품가공과)
나영미 (건국대학교 축산가공학과)
김창한 (건국대학교 축산가공학과)
Publication Information
Food Science of Animal Resources / v.23, no.1, 2003 , pp. 80-85 More about this Journal
Abstract
This study was carried out to investigate antimicrobial and antitumoral activities of Candida kefyr TFP7 isolated from Tibetan fermented milk Strains of TFP1∼10 were isolated from Tibetan fermented milk by agar diffusion method using potato dextrose agar(PDA). Antimicrobial activities were examined against 18 microorganisms of food-related bacteria, yeast, algae, fungi and actinomycetes isolated from soil. Antitumor activities were examined against 9 human tumor cell lines. Strains of TFP2∼10 showed strong antimicrobial activities against Micrococcus luteus ATCC l1880, and strains of TFP6∼10 to actinomycetes, Streptomyces murinus JCM 4333. In antitumor test, all isolated strains(TEP1∼10) showed the growth inhibition of SNU-5 and SW-534 by 60% and 70%, respectively. Among those, the strain TFP7 showed the most antitumor activity, which was 77.5% for SNU-5 and 76.5% for SW-534. The strain was identified as Candida kefyr by use of API 20C AUX kit and scanning electron micrograph.
Keywords
antimicrobial activity; antitumor activity; candida kefyr; tibetan fermented milk;
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1 K.oroleva, N. S. and Bavina, N. A. (1970) Effects of conditions of kefir fungus cultivation on the microflora and biochemical characteristics of kefir starters. Proc. 8th Int. Dairy Cong 1E., 413
2 Na, Y. M. (2003) Antimicrobial activity and Antitumor activity by Cundida kefyr TFP7 Isolated from Tibetan Fermented Milk. M. S. thesis, K.onkuk Univ., Seoul, Korea
3 Wiseman, U. W. and Marth, E. H. (1983) Behavior of aflatoxin Ml in yogurt, butter milk and kefir. J. Food Protection 46(2), 115-118   DOI
4 Allcroft, R. and Camaghan, R. B. A. (1962) Groundnut toxicity : An examination tbr toxin in human products from animals ted toxic groundnut meal. Vet. Rec. 75, 259-263
5 K.oneman, E. W., Aelen, S. D., Dowell, V. R., and Sommers, H. M. (1979) Color atlas and textbook of diagnostic microbiology. Lippincott Co., Philadelphia. Toronto, pp. 316-349
6 K.andler, 0. and Kunath, P. (1983) LactobaciUus kefir sp. nov., A component of the microflora of kefir. Systematic and Applied Microbiolovy 4, 286-294   DOI
7 Alley, M. C., Scudiero, D. A., Monks, A., Hursley, M. L.,Czerwinski, M. J., Fine, D. L., Abbott, B. J., Mayo, J. G., Shoemaker, R. H., and Boyd, M. R. (1988) Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Research 48, 589-601
8 Lee, K. H. (2000) Studies on antitumor, antileukemic and apoptosis induction activities of di(2-ethy1hexy1)Phtha1ate isolated from Aloe veru Linne. Ph. D. thesis. Konkuk Univ., Seoul, Korea
9 Nakanishi,T.(1967)牛乳と乳製品の微微生物.pp.157
10 Nagaoka, M., Muto, M., Nomto, K.., Matuzaki, M., Watanabe, T., and Yokokura, T. (1990) Structure of Polysaccharide Peptidogly-can Complex from the Cell Wall of LactohacitIus casei Y[T 9018. J. Biochem. 108, 568-571   DOI   PUBMED
11 Suganuma, M., Ohkura, Y., Okabe, S., and Fujiki, H. (2001)Combination cancer chemoprevention with green tea extract and sulindac shown in intestinal tumor formation in Min mice. Journul of Cancer Research and Ctinical OncoIoyy 127, 69-72   DOI   ScienceOn
12 Yan, M., Lin, H., Shen, Y., and Wang, Q. (2001) Studies on inhibiting activities of five antitumour drugs to human cancer cell in vitro with MTT assay. Jourwl of Chinese Medicinal Materials24, 418-419
13 Bottazzi, V. (1980) A note on scanning electron microscopy of micro-organisms associated with the kefir granule. J. Applivd Bacteriolom 48, 265-268   DOI
14 Choi, G. H. and Kim, C. H. (2002) Growth inhibition of extracts from sulfur feed duck meat against various tumor cell lines. Kor. J. Food Sci. Ani. Resour. 22, 348-351
15 Yang, S. J., Yoon, J. W., Seo, K-. S., K-oo, H. C., Kim. S. H., Bae, H. S., Baek, Y. and Park, Y. H. (1999) Prophylactic KfTects of Bifidobacterium Iongum HY8001 against Escherichia coli 0157 :117 and Satmonella typhiimurium DT104 Enteric Infection and Evaluation of Vero Cytotoxin Neutralizing Effccts. Kor. J. Appl. Microhiot. Biolechiwl. Vol. 27, No. 5, 419-425
16 Ham, J. S., Jeong, J. Y., Jeong, S. G., Ahn, J. N., Ahn, Y. T., K-im, S. K... and Kim, H. V. (1999) Effects of Feeding with Lactobacillus ptantarum and Candida kefyr Isolated from Mongolian Koumiss on the Growth and Fecal Microflora of Broilers. Korean J. Dairy Sci. 21(3) : 241-246