Browse > Article
http://dx.doi.org/10.5713/ab.22.0043

Impact of co-transfer of embryos produced by somatic cell nuclear transfer using two types of donor cells on pregnancy outcomes in dogs  

Son, Young-Bum (Abu Dhabi Research Foundation)
Jeong, Yeon Ik (Abu Dhabi Research Foundation)
Jeong, Yeon Woo (Abu Dhabi Research Foundation)
Hossein, Mohammad Shamim (Abu Dhabi Research Foundation)
Hwang, Woo Suk (Abu Dhabi Research Foundation)
Publication Information
Animal Bioscience / v.35, no.9, 2022 , pp. 1360-1366 More about this Journal
Abstract
Objective: The present study analyzed the influence of co-transferring embryos with high and low cloning efficiencies produced via somatic cell nuclear transfer (SCNT) on pregnancy outcomes in dogs. Methods: Cloned dogs were produced by SCNT using donor cells derived from a Tibetan Mastiff (TM) and Toy Poodle (TP). The in vivo developmental capacity of cloned embryos was evaluated. The pregnancy and parturition rates were determined following single transfer of 284 fused oocytes into 21 surrogates and co-transfer of 47 fused oocytes into four surrogates. Results: When cloned embryos produced using a single type of donor cell were transferred into surrogates, the pregnancy and live birth rates were significantly higher following transfer of embryos produced using TP donor cells than following transfer of embryos produced using TM donor cells. Next, pregnancy and live birth rates were compared following single and co-transfer of these cloned embryos. The pregnancy and live birth rates were similar upon co-transfer of embryos and single transfer of embryos produced using TP donor cells but were significantly lower upon single transfer of embryos produced using TM donor cells. Furthermore, the parturition rate for TM dogs and the percentage of these dogs that remained alive until weaning was significantly higher upon co-transfer than upon single transfer of embryos. However, there was no difference between the two embryo transfer methods for TP dogs. The mean birth weight of cloned TM dogs was significantly higher upon single transfer than upon co-transfer of embryos. However, the body weight of TM dogs did not significantly differ between the two embryo transfer methods after day 5. Conclusion: For cloned embryos with a lower developmental competence, the parturition rate and percentage of dogs that remain alive until weaning are increased when they are co-transferred with cloned embryos with a greater developmental competence.
Keywords
Co-transfer; Dog; Pregnancy Outcome; Single Transfer; Somatic Cell Nuclear Transfer;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Tian XC, Kubota C, Enright B, Yang X. Cloning animals by somatic cell nuclear transfer--biological factors. Reprod Biol Endocrinol 2003;1:98. https://doi.org/10.1186/1477-7827-1-98   DOI
2 Son YB, Jeong YI, Jeong YW, et al. Comparison of pregnancy outcomes following the transfer of early-developmental stage embryos and blastocysts produced by somatic cell nuclear transfer in Camelus dromedarius. Anim Reprod Sci 2021; 233:106842. https://doi.org/10.1016/j.anireprosci.2021.106842   DOI
3 Kim MJ, Oh HJ, Park JE, et al. Influence of oocyte donor and embryo recipient conditions on cloning efficiency in dogs. Theriogenology 2010;74:473-8. https://doi.org/10.1016/j.theriogenology.2010.03.001   DOI
4 Meng Q, Wang M, Stanca CA, Bodo S, Dinnyes A. Cotransfer of parthenogenetic embryos improves the pregnancy and implantation of nuclear transfer embryos in mouse. Cloning Stem Cells 2008;10:429-34. https://doi.org/10.1089/clo.2008.0003   DOI
5 Greco E, Litwicka K, Ferrero S, et al. Co-transfer of embryos derived from cryopreserved and fresh natural cycle oocytes: a pilot study. Reprod Biomed Online 2008;17:530-6. https://doi.org/10.1016/s1472-6483(10)60241-6   DOI
6 Wilmut I, Beaujean N, de Sousa PA, et al. Somatic cell nuclear transfer. Nature 2002;419:583-7. https://doi.org/10.1038/nature01079   DOI
7 Kasai K, Sano F, Miyashita N, Watanabe S, Nagai T. Comparison of the growth performances of offspring produced by a pair of cloned cattle and their nuclear donor animals. J Reprod Dev 2007;53:135-42. https://doi.org/10.1262/jrd.18063   DOI
8 Lee SH, Oh HJ, Kim MJ, et al. Oocyte maturation-related gene expression in the canine oviduct, cumulus cells, and oocytes and effect of co-culture with oviduct cells on in vitro maturation of oocytes. J Assist Reprod Genet 2017;34:929-38. https://doi.org/10.1007/s10815-017-0910-x   DOI
9 Jeong YW, Kim JJ, Kim HD, et al. Preimplantation development of cloned canine embryos recovered by hysterectomy or surgical uterine flushing and subsequent pregnancy outcomes. Theriogenology 2016;86:1865-72. https://doi.org/10.1016/j.theriogenology.2015.10.026   DOI
10 Jeong YW, Lee GS, Kim JJ, et al. Establishment of a canine model of human type 2 diabetes mellitus by overexpressing phosphoenolypyruvate carboxykinase. Int J Mol Med 2012; 30:321-9. https://doi.org/10.3892/ijmm.2012.993   DOI
11 Park KS, Kim KJ, Choi SA, et al. 322 Induction of superovulation in proesturs dogs using serum gonadotropin of pregnant mares and human chorionic gonadotropin. Reprod Fertil Dev 2010;23:257-8. https://doi.org/10.1071/RDv23n1Ab322   DOI
12 Kim GA, Oh HJ, Park JE, et al. Employing mated females as recipients for transfer of cloned dog embryos. Reprod Fertil Dev 2013;25:700-6. https://doi.org/10.1071/RD11221   DOI
13 Kato Y, Tani T, Sotomaru Y, et al. Eight calves cloned from somatic cells of a single adult. Science 1998;282:2095-8. https://doi.org/10.1126/science.282.5396.2095   DOI
14 Li J, Du M, Zhang Z, et al. Does a poor-quality embryo have an adverse impact on a good-quality embryo when transferred together? J Ovarian Res 2018;11:78. https://doi.org/10.1186/s13048-018-0452-6   DOI
15 Grupen CG. The evolution of porcine embryo in vitro production. Theriogenology 2014;81:24-37. https://doi.org/10.1016/j.theriogenology.2013.09.022   DOI
16 Son YB, Jeong YI, Hossein MS, et al. Comparative evaluation of three different formulas for predicting the parturition date of German Shepherds following somatic cell nuclear transfer. J Vet Med Sci 2021;83:1448-53. https://doi.org/10.1292/jvms.21-0154   DOI
17 Sutsui T, Hori T, Okazaki H, et al. Transfer of canine embryos at various developmental stages recovered by hysterectomy or surgical uterine flushing. J Vet Med Sci 2001;63:401-5. https://doi.org/10.1292/jvms.63.401   DOI
18 Liu J, Wang Y, Su J, Luo Y, Quan F, Zhang Y. Nuclear donor cell lines considerably influence cloning efficiency and the incidence of large offspring syndrome in bovine somatic cell nuclear transfer. Reprod Domest Anim 2013;48:660-4. https://doi.org/10.1111/rda.12140   DOI
19 Aldemir O, Ozelci R, Baser E, et al. Impact of transferring a poor quality embryo along with a good quality embryo on pregnancy outcomes in IVF/ICSI Cycles: a retrospective study. Geburtshilfe Frauenheilkd 2020;80:844-50. https://doi.org/10.1055/a-1213-9164   DOI
20 McLernon DJ, Harrild K, Bergh C, et al. Clinical effectiveness of elective single versus double embryo transfer: meta-analysis of individual patient data from randomised trials. BMJ 2010; 341:c6945. https://doi.org/10.1136/bmj.c6945   DOI
21 Hossein MS, Jeong YW, Kim S, et al. Protocol for the recovery of in vivo matured canine oocytes based on once daily measurement of serum progesterone. Cloning Stem Cells 2008; 10:403-8. https://doi.org/10.1089/clo.2008.0001   DOI
22 Kawarasaki T, Otake M, Tsuchiya S, Shibata M, Matsumoto K, Isobe N. Co-transfer of parthenogenotes and single porcine embryos leads to full-term development of the embryos. Anim Reprod Sci 2009;112:8-21. https://doi.org/10.1016/j.anireprosci.2008.03.022   DOI
23 Tamashiro KL, Wakayama T, Blanchard RJ, Blanchard DC, Yanagimachi R. Postnatal growth and behavioral development of mice cloned from adult cumulus cells. Biol Reprod 2000;63:328-34. https://doi.org/10.1095/biolreprod63.1.328   DOI
24 Palmieri C, Loi P, Ptak G, Della Salda L. Review paper: a review of the pathology of abnormal placentae of somatic cell nuclear transfer clone pregnancies in cattle, sheep, and mice. Vet Pathol 2008;45:865-80. https://doi.org/10.1354/vp.45-6-865   DOI
25 Jeong YW, Kim JJ, Hossein MS, et al. Influence of somatic cell donor breed on reproductive performance and comparison of prenatal growth in cloned canines. Theriogenology 2014;81: 1207-13. https://doi.org/10.1016/j.theriogenology.2014.01.036   DOI
26 Lee GS, Jeong YW, Kim JJ, et al. A canine model of Alzheimer's disease generated by overexpressing a mutated human amyloid precursor protein. Int J Mol Med 2014;33:1003-12. https://doi.org/10.3892/ijmm.2014.1636   DOI
27 Wei YF, Wan JS, Chen FL, et al. A retrospective study of female reproductive parameters in the Kunming dog. Anim Sci J 2018;89:52-9. https://doi.org/10.1111/asj.12914   DOI
28 Reynaud K, Fontbonne A, Marseloo N, et al. In vivo meiotic resumption, fertilization and early embryonic development in the bitch. Reproduction 2005;130:193-201. https://doi.org/10.1530/rep.1.00500   DOI
29 Son YB, Jeong YI, Hwang KC, Jeong YW, Hwang WS. Mitochondrial metabolism assessment of lycaon-dog fetuses in interspecies somatic cell nuclear transfer. Theriogenology 2021;165:18-27. https://doi.org/10.1016/j.theriogenology.2021.01.010   DOI
30 Kim JJ, Park KB, Choi EJ, et al. Relationship between time post-ovulation and progesterone on oocyte maturation and pregnancy in canine cloning. Anim Reprod Sci 2017;185: 75-82. https://doi.org/10.1016/j.anireprosci.2017.08.004   DOI