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http://dx.doi.org/10.5713/ab.21.0131

Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes  

Son, Young-Bum (UAE Biotech Research Center)
Jeong, Yeon Ik (UAE Biotech Research Center)
Jeong, Yeon Woo (UAE Biotech Research Center)
Olsson, Per Olof (UAE Biotech Research Center)
Hossein, Mohammad Shamim (UAE Biotech Research Center)
Cai, Lian (UAE Biotech Research Center)
Kim, Sun (UAE Biotech Research Center)
Choi, Eun Ji (UAE Biotech Research Center)
Sakaguchi, Kenichiro (UAE Biotech Research Center)
Tinson, Alex (Hilli E.T. Cloning and Surgical Centre Presidential Camels and Camel Racing Affairs)
Singh, Kuhad Kuldip (Hilli E.T. Cloning and Surgical Centre Presidential Camels and Camel Racing Affairs)
Rajesh, Singh (Hilli E.T. Cloning and Surgical Centre Presidential Camels and Camel Racing Affairs)
Noura, Al Shamsi (Hilli E.T. Cloning and Surgical Centre Presidential Camels and Camel Racing Affairs)
Hwang, Woo Suk (UAE Biotech Research Center)
Publication Information
Animal Bioscience / v.35, no.2, 2022 , pp. 177-183 More about this Journal
Abstract
Objective: The present study evaluated the efficiency of embryo development and pregnancy of somatic cell nuclear transfer (SCNT) embryos using different source-matured oocytes in Camelus dromedarius. Methods: Camelus dromedarius embryos were produced by SCNT using in vivo- and in vitro- matured oocytes. In vitro embryo developmental capacity of reconstructed embryos was evaluated. To confirm the efficiency of pregnancy and live birth rates, a total of 72 blastocysts using in vitro- matured oocytes transferred into 45 surrogates and 95 blastocysts using in vivo- matured oocytes were transferred into 62 surrogates by transvaginal method. Results: The collected oocytes derived from ovum pick up showed higher maturation potential into metaphase II oocytes than oocytes from the slaughterhouse. The competence of cleavage, and blastocyst were also significantly higher in in vivo- matured oocytes than in vitro- matured oocytes. After embryo transfer, 11 pregnant and 10 live births were confirmed in in vivo- matured oocytes group, and 2 pregnant and 1 live birth were confirmed in in vitro- matured oocytes group. Furthermore, blastocysts produced by in vivo-matured oocytes resulted in significantly higher early pregnancy and live birth rates than in vitro-matured oocytes. Conclusion: In this study, SCNT embryos using in vivo- and in vitro-matured camel oocytes were successfully developed, and pregnancy was established in recipient camels. We also confirmed that in vivo-matured oocytes improved the development of embryos and the pregnancy capacity using the blastocyst embryo transfer method.
Keywords
Camelus dromedarius; Embryo Development; In vitro-matured Oocytes; In vivo-Matured Oocytes; Pregnancy Rates; Somatic Cell Nuclear Transfer;
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