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http://dx.doi.org/10.5713/ajas.20.0545

Application of lactic acid bacteria producing antifungal substance and carboxylesterase on whole crop rice silage with different dry matter  

Lee, Seong Shin (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University)
Paradhipta, Dimas Hand Vidya (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University)
Lee, Hyuk Jun (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University)
Joo, Young Ho (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University)
Noh, Hyeon Tak (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University)
Choi, Jeong Seok (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University)
Ji, Keum Bae (Institute of Technology, Livemac Co. Ltd.)
Kim, Sam Churl (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University)
Publication Information
Animal Bioscience / v.34, no.6, 2021 , pp. 1029-1037 More about this Journal
Abstract
Objective: This study was conducted to investigate effects of antifungal substance and carboxylesterase-producing inoculant on fermentation indices and rumen degradation kinetics of whole crop rice (WCR) silage ensiled at different dry matter (DM) contents. Methods: Dual-purpose inoculants, Lactobacillus brevis 5M2 and Lactobacillus buchneri 6M1, confirmed both activities of antifungal and carboxylesterase in the previous study. The WCR at mature stage was chopped, and then wilted to obtain three different DM contents consisting of 35.4%, 43.6%, and 51.5%. All WCR forages were applied distilled water (CON) or mixed inoculants with 1:1 ratio at 1×105 colony forming unit/g (INO), and ensiled into 20 L mini silo (5 kg) in quadruplicates for 108 d. Results: The INO silages had lower lactate (p<0.001) and butyrate (p = 0.022) with higher acetate (p<0.001) and propionate (p<0.001) than those of CON silages. Ammonia-N (p<0.001), lactate (tendency; p = 0.068), acetate (p = 0.030), and butyrate (p<0.001) concentrations of INO silages decreased linearly with increasing DM content of WCR forage. The INO silages presented higher lactic acid bacteria (p<0.001) with lower molds (p<0.001) than those of CON silages. Yeasts (p = 0.042) and molds (p = 0.046) of WCR silages decreased linearly with increasing DM content of WCR forage. In the rumen, INO silages had higher the total degradable fraction (p<0.001), total volatile fatty acid (tendency; p = 0.097), and acetate (p = 0.007), but lower the fractional degradation rate (p = 0.011) and propionate (p<0.001) than those of CON silage. The total degradable fraction (p<0.001), total volatile fatty acid (p = 0.001), iso-butyrate (p = 0.036), and valerate (p = 0.008) decreased linearly with increasing DM content of WCR forage, while the lag phase (p<0.001) was increased linearly. Conclusion: This study concluded that application of dual-purpose inoculants on WCR silage confirmed antifungal and carboxylesterase activities by inhibiting mold and improving rumen digestibility, while increase of wilting times decreased organic acids production and rumen digestibility.
Keywords
Haylage; Lactic Acid Bacteria; Rumen Fermentation; Silage; Whole Crop Rice;
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1 Nkosi BD, Meeske R. Effects of ensiling totally mixed potato hash ration with or without a heterofermentative bacterial inoculant on silage fermentation, aerobic stability, growth performance and digestibility in lambs. Anim Feed Sci Technol 2010;161:38-48. https://doi.org/10.1016/j.anifeedsci.2010.07.015   DOI
2 Lee SS, Lee HJ, Paradhipta DHV, et al. Temperature and microbial changes of corn silage during aerobic exposure. Asian-Australas J Anim Sci 2019;32:988-95. https://doi.org/10.5713/ajas.18.0566   DOI
3 Han KJ, Collins M, Vanzant ES, Dougherty CT. Bale density and moisture effects on alfalfa round bale silage. Crop Sci 2004;44:914-9. https://doi.org/10.2135/cropsci2004.9140   DOI
4 Kleinschmit DH, Schmidt RJ, Kung L. The effects of various antifungal additives on the fermentation and aerobic stability of corn silage. J Dairy Sci 2005;88:2130-9. https://doi.org/10.3168/jds.S0022-0302(05)72889-7   DOI
5 Hobson PN, Stewart CS. The rumen microbial ecosystem, 2nd ed. London, UK: Blackie Academic and Professional; 1997. pp. 543-708.
6 Weinberg ZG, Chen Y, Gamburg M. The passage of lactic acid bacteria from silage into rumen fluid, in vitro studies. J Dairy Sci 2004;87:3386-97. https://doi.org/10.3168/jds.S0022-0302(04)73474-8   DOI
7 Sutton JD, Dhanoa MS, Morant SV, France J, Napper DJ, Schuller E. Rates of production of acetate, propionate, and butyrate in the rumen of lactating dairy cows given normal and low-roughage diets. J Dairy Sci 2003;86:3620-33. https://doi.org/10.3168/jds.S0022-0302(03)73968-X   DOI
8 Paradhipta DHV, Lee SS, Kang B, et al. Dual-purpose inoculants and their effects on corn silage. Microorganisms 2020;8:765. https://doi.org/10.3390/microorganisms8050765   DOI
9 Santos MC, Kung L. Short communication: the effects of dry matter and length of storage on the composition and nutritive value of alfalfa silage. J Dairy Sci 2016;99:5466-9. https://doi.org/10.3168/jds.2016-10866   DOI
10 Paradhipta DHV, Joo YH, Lee HJ, et al. Effects of inoculant application on fermentation quality and rumen digestibility of high moisture sorghum-sudangrass silage. J Appl Anim Res 2019;47:486-91. https://doi.org/10.1080/09712119.2019.1670667   DOI
11 Alonso VA, Pereyra CM, Keller LAM, et al. Fungi and mycotoxins in silage: an overview. J Appl Microbiol 2013;115:637-43. https://doi.org/10.1111/jam.12178   DOI
12 Adesogan AT, Ma ZX, Romero JJ, Arriola KG. Ruminant nutrition symposium: improving cell wall digestion and animal performance with fibrolytic enzymes. J Anim Sci 2014;92:1317-30. https://doi.org/10.2527/jas.2013-7273   DOI
13 Paradhipta DHV, Joo YH, Lee HJ, et al. Effects of wild or mutated inoculants on rye silage and its rumen fermentation indices. Asian-Australas J Anim Sci 2020;33:949-56. https://doi.org/10.5713/ajas.19.0308   DOI
14 Schnurer J, Magnusson J. Antifungal lactic acid bacteria as biopreservatives. Trends Food Sci Technol 2005;16:70-8. https://doi.org/10.1016/j.tifs.2004.02.014   DOI
15 Muck RE, Dickerson JT. Storage temperature effects on proteolysis in alfalfa silage. Trans ASAE 1988;31:1005-9. https://doi.org/10.13031/2013.30813   DOI
16 Aquino D, Del Barrio A, Trach NX, et al. Rice straw-based fodder for ruminants. In: Gummert M, Van Hung N, Chivenge P, Douthwaite B, editors. Sustainable rice straw management. Cham, Switzerland: Springer; 2020. pp. 111-29.
17 Zhao J, Dong Z, Li J, et al. Effects of sugar sources and doses on fermentation dynamics, carbohydrates changes, in vitro digestibility and gas production of rice straw silage. Ital J Anim Sci 2019;18:1345-55. https://doi.org/10.1080/1828051X.2019.1659106   DOI
18 McDonald P, Henderson AR, Heron SJE. The biochemistry of silage, 2nd ed. Marlow, Bucks, UK: Chalcombe Publications; 1991. pp. 248-91.
19 AOAC International. Official methods of analysis. 18th ed. Washington DC, USA: AOAC International; 2005.
20 Chaney AL, Marbach EP. Modified reagents for determination of urea and ammonia. Clin Chem 1962;8:130-2. https://doi.org/10.1093/clinchem/8.2.130   DOI
21 Adesogan AT, Krueger NK, Kim SC. A novel, wireless, automated system for measuring fermentation gas production kinetics of feeds and its application to feed characterization. Anim Feed Sci Technol 2005;123-4:211-23. https://doi.org/10.1016/j.anifeedsci.2005.04.058   DOI
22 SAS Institute Inc. SAS/STAT User's guide: version 9. Cary, NC, USA: SAS Institute Inc.; 2002.
23 McDonald I. A revised model for the estimation of protein degradability in the rumen. J Agric Sci 1981;96:251-2. https://doi.org/10.1017/S0021859600032081   DOI
24 Kim JG, Chung ES, Seo S, et al. Effect of growth stage and variety on the quality of whole crop rice silage. J Korean Soc Grassl Forage Sci 2008;28:29-34. https://doi.org/10.5333/KGFS.2008.28.1.029   DOI
25 Wanapat M, Kang S, Khejornsart P, Pilajun R, Wanapat S. Performance of tropical dairy cows fed whole crop rice silage with varying levels of concentrate. Trop Anim Health Prod 2014;46:185-9. https://doi.org/10.1007/s11250-013-0473-5   DOI
26 Takahashi T, Horiguchi K, Goto M. Effect of crushing unhulled rice and the addition of fermented juice of epiphytic lactic acid bacteria on the fermentation quality of whole crop rice silage, and its digestibility and rumen fermentation status in sheep. Anim Sci J 2005;76:353-8. https://doi.org/10.1111/j.1740-0929.2005.00275.x   DOI
27 Boufaied H, Chouinard PY, Tremblay GF, Petit HV, Michaud R, Belanger G. Fatty acids in forages. I. Factors affecting concentrations. Can J Anim Sci 2003;83:501-11. https://doi.org/10.4141/A02-098   DOI
28 Roffler RE, Niedermeier RP, Baumgardt BR. Evaluation of alfalfa-brome forage stored as wilted silage, low-moisture silage, and hay. J Dairy Sci 1967;50:1805-13. https://doi.org/10.3168/jds.S0022-0302(67)87719-1   DOI
29 Kim BW, Kim GS, Sung KI. Effect of lactic acid bacteria and formic acid on the silage quality of whole crop rice at different maturity. J Korean Soc Grassl Forage Sci 2004;24:61-70. https://doi.org/10.5333/KGFS.2004.24.1.061   DOI
30 Danner H, Holzer M, Mayrhuber E, Braun R. Acetic acid increases stability of silage under aerobic conditions. Appl Environ Microbiol 2003;69:562-7. https://doi.org/10.1128/AEM.69.1.562-567.2003   DOI