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http://dx.doi.org/10.5713/ajas.20.0349

Protective effects of 5-aminolevulinic acid on heat stress in bovine mammary epithelial cells  

Islam, Md Aminul (Graduate School of Medicine, Science and Technology, Shinshu University)
Noguchi, Yoko (Neopharma Japan Co., Ltd.)
Taniguchi, Shin (Neopharma Japan Co., Ltd.)
Yonekura, Shinichi (Graduate School of Medicine, Science and Technology, Shinshu University)
Publication Information
Animal Bioscience / v.34, no.6, 2021 , pp. 1006-1013 More about this Journal
Abstract
Objective: Cells have increased susceptibility to activation of apoptosis when suffering heat stress (HS). An effective supplementation strategy to mimic heat-induced apoptosis of bovine mammary epithelial cells (MECs) is necessary to maintain optimal milk production. This study aimed to investigate possible protective effects of the anti-apoptotic activity of 5-aminolevulinic acid (5-ALA) against HS-induced damage of bovine MECs. Methods: Bovine MECs were pretreated with or without 5-ALA at concentrations of 10, 100, and 500 µM for 24 h followed by HS (42.5℃ for 24 h and 48 h). Cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Real-time quantitative polymerase chain reaction and Western blotting were used to explore the regulation of genes associated with apoptosis, oxidative stress, and endoplasmic reticulum (ER) stress genes. Results: We found that 5-ALA induces cytoprotection via inhibition of apoptosis markers after HS-induced damage. Pretreatment of bovine MECs with 5-ALA resulted in dramatic upregulation of mRNA for nuclear factor erythroid-derived 2-like factor 2, heme oxygenase-1, and NAD(P)H quinone oxidoreductase 1, all of which are antioxidant stress genes. Moreover, 5-ALA pretreatment significantly suppressed HS-induced ER stress-associated markers, glucose-regulated protein 78, and C/EBP homologous protein expression levels. Conclusion: 5-ALA can ameliorate the ER stress in heat stressed bovine MEC via enhancing the expression of antioxidant gene.
Keywords
Heat Stress; 5-Aminolevulinic Acid; Bovine Mammary Epithelial Cells; Endoplasmic Reticulum Stress; Oxidative Stress;
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