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http://dx.doi.org/10.9708/jksci.2022.27.01.175

Anti-inflammatory Effect of Broccoli Leaf Hexane Fraction in LPS-stimulated RAW264.7 Cells  

Kim, Mee-Kyung (Dept. of Bio-Cosmetic Science, Seowon University)
Abstract
In this study, we tested the anti-inflammatory effects of broccoli leaf hexane fraction to confirm the applicability as a functional material in food and cosmetics. This sample was extracted using 70% ethanol from Broccoli leaf and then fractionated with hexane. The production of pro-inflammatory cytokines (TNF-α, IL-4, IL-6, IL-1β), protein expression of iNOS and COX-2, phosphorylation of MAPKs (ERK, JNK, p38) and NF-κB with broccoli leaf hexane fraction were assayed on LPS-stimulated RAW264.7 cells. The broccoli leaf hexane fraction inhibited the secretion of pro-inflammatory cytokines and protein expression of iNOS and COX-2. Also, the broccoli leaf hexane fraction reduced the phosphorylation of MAPKs and NF-κB. Therefore, it is considered that th broccoli leaf hexane fraction has the potential to be used as a natural anti-inflammatory material in food and cosmetics. In the future, it is considered necessary to study the anti-inflammatory mechanism and identification of major bioactive substances.
Keywords
Anti-inflammatory effect; Broccoli leaf; iNOS & COX-2; MAPKs; NF-${\kappa}B$;
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