Browse > Article
http://dx.doi.org/10.7744/kjoas.20160023

Acute toxicity evaluation of drought-tolerant transgenic rice Agb0103 to Daphnia magna  

Oh, Sung-Dug (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Bum Kyu (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
Park, Soo-Yun (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
Yun, Doh-Won (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
Sohn, Soo-In (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
Chang, Ancheol (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
Suh, Sang Jae (School of Applied Biosciences, Kyungpook National University)
Publication Information
Korean Journal of Agricultural Science / v.43, no.2, 2016 , pp. 205-214 More about this Journal
Abstract
A drought-tolerant transgenic rice (Agb0103) was developed using a pepper methionine sulfoxide reductase (CaMsrB2) under the control of rice Rab21 promoter with a selection marker, the phosphinothricin acetyltransferase (PAT) gene. Commercialization of genetically modified (GM) crops will require the evaluation of risks associated with the release of GM crops. With the potential problems associated to GM crops safety testing, the investigation of their effects on non-target organisms is necessary for environmental risk research. This study was carried out to assess acute toxicity of a GM crop using the water flea (Daphnia magna) for non-target organism risk evaluation. The effect of acute toxicity on Daphnia magna of Agb0103 rice and a non-GM rice, Ilmibyeo, were investigated at different concentrations (0, 625, 1,250, 2,500, 5,000, and 10,000 mg/L). The Agb0103 rice used for the test was confirmed to express the CaMsrB2/PAT gene by the PCR and ELISA. Daphnia magna feeding tests showed no significant differences in cumulative immobility or abnormal response with either Agb0103 rice or non-GM rice. The 48hr-EC50 values showed no difference between Agb0103 rice (2243 mg/L) and non-GM rice (2694 mg/L). These results suggest that there is no significant difference in toxicity to Daphnia magna between Agb0103 rice and its non-GM counterpart.
Keywords
Daphnia magna; drought-tolerant transgenic rice; risk assessment;
Citations & Related Records
Times Cited By KSCI : 9  (Citation Analysis)
연도 인용수 순위
1 Ha SH, Liang YS, Jung HR, Ahn MJ, Suh GC, Kweon SJ, Kim DH, Kim YM, Kim JK. 2010. Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm. Plant Biotechnology 8:928-938.   DOI
2 James C. 2014. The global status of commercialized biotech/GM crops: 2014. ISAAA Brief No. 49. ISAAA: Ithaca, NY.
3 Jung S, Lee Y, Yang K, Lee SB, Jang SM, Ha SB, Back K. 2004. Dual targeting of Myxococcus xanthus protoporphyrinogen oxidase into chloroplasts and mitochondria and high level oxyfluorfen resistance. Plant Cell Environment 27:1436-1446.   DOI
4 Kim JS, Park HM, Chae SH, Lee TH, Hwang DJ, Oh SD, Song DG, Park JS, Pan CH, Nahm BH, Kim YH, Kim YK. 2014. A pepper Msrb2 gene confers drought tolerance in rice through the protection of chloroplast targeted genes. Plos one 9:e90588.   DOI
5 Kim HJ, Lee SM, Kim JK, Ryu TH, Suh SC, Cho HS. 2010. Expression of PAT and NPT II proteins during the developmental stages of a genetically modified pepper developed in Korea. Journal of Agriculture & Food Chemistry 58:10906-10910.   DOI
6 Lee CW, Ryu JY, Lim KW. 2007. Acute toxicity test of agricultural chemicals to water fleas. Journal of the Environmental Sciences 16:55-63. [in Korean]   DOI
7 Lee SM, Park SY, Yeo YS, Cho HS, Kwon SJ, Oh SD, Oh SW, Kim JK, Park SK. 2015. The allergy assessments of insect-resistant Agb0101 rice. Korean Journal International Agriculture 27:69-75. [in Korean]
8 Li G, Wang Y, Liu B, Zhang G. 2014. Transgenic Bacillus thuringiensis (Bt) rice is safer to aquatic ecosystems than its non-transgenic counterpart. Plos one. 9:e104270.   DOI
9 Moon JH, Moon JM, Lee YH, Lee JJ. 2013. Influence of transgenic rice plant transformed with silbene synthase gene on insect fauna and biosafety evaluation on biological indicators. Journal of Agriculture & Life Science 44:11-16. [in Korean]
10 Oh SD, Shin HC, Sohn SI, Lee KJ, Kim HJ, Ryu TH, Lee JY, Park BS, Kweon SJ, Suh SC, Park JS. 2011. Evaluation and assessment of biosafety for Bt-transgenic rice: Responses of Daphnia magna fed on Bt-transgenic rice variety, Journal of Applied Biological Chemistry 54:296-302. [in Korean]   DOI
11 Oh SD, Lee KJ, Park SY, Sohn SI, Ryu TH, Kim JK, Kim JS, Ahn HI, Ha SH, Park JS, Ahn BO, Cho HS, Suh SJ. 2012. Molecular biological analysis of carotenoid-biofortified rice and its effect on Daphnia magna feeding. Korean Journal International Agriculture 24:477-484. [in Korean]
12 Oh SD, Lee KJ, Park SY, Lee DY, Sohn SI, Kim MY, Ryu TH. 2013. Responses of Misgurnus anguillicaudatus and Cyprinus carpio fed on disease resistant (OsCK1) rice variety. Korean Journal of Environmental Agriculture 32:231-239. [in Korean]   DOI
13 Oh SD, Lee KJ, Park SY. Ryu TH, Suh SJ. 2014a. Acute toxicity evaluation to Daphnia magna of disease resistant (OsCK1) rice. Korean Journal of Environmental Agriculture 33:121-128. [in Korean]   DOI
14 Thomas, B, Traavik T, Primicerio R. 2010. Demographic responses of Daphnia magna fed transgenic Bt-maize. Ecotoxicology 19:419-430.   DOI
15 Oh SD, Sohn SI, Lee KJ, Shin HC, Park JS, Ryu TH. 2014b. Risk assessment and evaluation of carotenoid-biofortified rice: responses of Misgurnus Anguillicaudatus and Cyprinus Carpio fed on carotenoid-biofortified rice variety. Korean Journal International Agriculture 26:27-34. [in Korean]   DOI
16 Oh SK, Baek KH, Seong ES, Joung YH, Choi GJ, Park JM, Cho HS, Kim EA, Lee S, Choi DI. 2010. CaMsrB2, pepper methionine sulfoxide reductase B2, is a novel defense regulator against oxidative stress and pathogen attack. Plant Physiology. 154:245-261.   DOI
17 Shin KS, Lee SM, Lim SH, Woo HJ, Cho HS, Lee KR, Lee MC, Kweon SJ, Suh SC. 2009. Molecular biological characteristics and analysis using the specific markers of leaf folder-resistant GM rice. Plant Biotechnology 36:115-123.   DOI
18 Toriyama K, Arimotoa Y, Uchimiyaa H, Hinata K. 1988. Transgenic rice plants after direct gene transfer into protoplasts. Nature Biotechnology 6:1072-1074.   DOI
19 Woo HJ, Shin KS, Lim MH, Lee JH, Qin Y, Park SK. 2015. Comparative nutritional analysis for marker-free transgenic Bt rice and non-transgenic counterparts. Korean Journal of Breeding Science 47:39-44. [in Korean]   DOI