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http://dx.doi.org/10.7744/cnujas.2014.41.3.187

Development of distinction methods for male-sterile and dwarfism herbicide tolerant Zoysia japonica Steud  

Lee, Bum Kyu (Biosafety Division, National Academy of Agricultural Science)
Kang, Hong-Gyu (Subtropical Horticulture Research Institute, Jeju National University)
Ra, Nu Ri (Biosafety Division, National Academy of Agricultural Science)
Sun, Hyeon-Jin (Subtropical Horticulture Research Institute, Jeju National University)
Kwon, Yong-Ik (Subtropical Horticulture Research Institute, Jeju National University)
Song, In-Ja (Subtropical Horticulture Research Institute, Jeju National University)
Kim, Chang-Gi (Bio-Evaluation Center, Korea Research Institute of Bioscience & Biotechnology)
Ryu, Tae-Hun (Biosafety Division, National Academy of Agricultural Science)
Park, Kee Woong (Department of Crop Science, Chungnam National University)
Lee, Hyo-Yeon (Subtropical Horticulture Research Institute, Jeju National University)
Publication Information
Korean Journal of Agricultural Science / v.41, no.3, 2014 , pp. 187-191 More about this Journal
Abstract
The cultivation area and use of genetically modified (GM) crops have been increased continuously over the world. Concerns about the potential risks of GM crops are also increasing. Safe management for the development and production of GM crops is required according to Living Modified Organism Act in Korea. Planning about the methods, duration, and frequency of environmental monitoring is also required for commercial use of GM crops. GM Zoysia japonica Steud. (event name: JG21) expressing resistance to glufosinate-ammonium has been generated previously. By using gamma ray treatment to JG21 we also developed male sterility and dwarf Z. japonica (event name: JG21-MS). The objective of this study was to establish the monitoring system for environment release of JG21-MS. In this study we extracted RNA from JG21 and JG21-MS and conducted RAPD (random amplified polymorphic DNA) method to distinguish JG21 and JG21-MS.
Keywords
Genetically modified (GM); Zoysia japonica; Environmental risk assessment; Monitoring; RAPD;
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