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http://dx.doi.org/10.7744/cnujas.2013.40.4.359

Reduction in antigenesity of whey protein by alcalase  

Yu, Jae-Min (Lab. of Milk Food Biochemistry and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
Renchinkhand, Renchinkhand (Lab. of Milk Food Biochemistry and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
Bae, Hyoung Churl (Lab. of Milk Food Biochemistry and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
Jeong, Seok Geun (National Institute of Animal Svience, Rural Development Administration)
Paik, Seung-Hee (Div. of Food Service Industry, Cheonan Yonam College)
Nam, Myoung Soo (Lab. of Milk Food Biochemistry and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
Korean Journal of Agricultural Science / v.40, no.4, 2013 , pp. 359-365 More about this Journal
Abstract
The aim of this study was to produce enzymatic hydrolysis of ${\alpha}$-LA, ${\beta}$-LG and BSA with alcalase for the possible application of hypoallergenic foods toward cow's milk allergenic infant. The molecular weights of most of the peptides in hydrolysates from ${\alpha}$-LA, ${\beta}$-LG and BSA by alcalase were below 3,000 dalton. Antigenesity of ${\alpha}$-LA, ${\beta}$-LG and BSA hydrolysates to rabbit anti-${\alpha}$-LA antiserum, ${\beta}$-LG antiserum and BSA antiserum were remarkably decreased by more than $10^{-3}$ at 20% inhibitionrate. Antigenesity of polyvalent antigenic peptide in ${\alpha}$-LA, ${\beta}$-LG and BSA hydrolysates to specific rabbit anti-${\alpha}$-LA antiserum, ${\beta}$-LG antiserum and BSA antiserum was determined by PCS test using guina-pig. Hydrolysates of ${\alpha}$-LA, ${\beta}$-LG and BSA with less than 3,000 dalton did not show polyvalent antigenic reaction against rabbit antiserum. Hydrolysates of ${\alpha}$-LA, ${\beta}$-LG and BSA could be a source for the manufacturing of hypoallergenic food.
Keywords
${\alpha}$-LA; ${\beta}$-LG; BSA; Enzymatic hydrolysis; Antigenesity;
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