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http://dx.doi.org/10.7744/cnujas.2013.40.3.221

Whey protein hydrolytic properties and its immunomodulation activity by produced enzyme from Serratia marcescens S3-R1  

Yu, Jae Min (Department of Animal Biosystem Science, College of Agriculture and Life Sciences, Chungnam National University)
Renchinkhand, G. (Department of Animal Biosystem Science, College of Agriculture and Life Sciences, Chungnam National University)
Jeong, Seok Geun (National Institute of Animal Science, Rural Development Administration)
Bae, Hyoung Churl (Department of Animal Biosystem Science, College of Agriculture and Life Sciences, Chungnam National University)
Nam, Myoung Soo (Department of Animal Biosystem Science, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
Korean Journal of Agricultural Science / v.40, no.3, 2013 , pp. 221-226 More about this Journal
Abstract
Degrees of hydrolysis by alkaline protease produced from Serratia marcescens S3-R1 is 3.95-6.30% of whey proteins during 5, 15, 30, 60, 90, 120,180, 240 min incubation at $40^{\circ}C$. Proteolytic pattern of the whey proteins showed that various low molecular weight peptides were generated during the incubation periods. The biological function of in Raw 264.7 cells treated with whey protein hydrolytic peptides, anti-inflammatory effect showed exhibit in the expression of pro-inflammatory cytokines such as TNF-${\alpha}$, IL-6, COX-2 and iNOS by PCR analysis. COX-2 and iNOS gene expression inhibited in Raw 264.7 cells on whey protein hydrolysates below 3,000 dalton. The protease from Serratia marcescens S3-R1 showed a potential in production of low molecular weight whey protein hydrolysates which could be used for industrial application.
Keywords
Serratia marcescens S3-R1; Protease; Peptide; Anti-inflammatory;
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