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http://dx.doi.org/10.7744/cnujas.2012.39.1.023

Molecular cloning of Prophenoloxidase (PPO) gene related to melanin formation of elytra of Harmonia axyridis  

Kim, Sae-Hee (Dept. Applied Biology, Chungnam National University)
Seo, Mi-Ja (Dept. Applied Biology, Chungnam National University)
Park, Min-Woo (Dept. Applied Biology, Chungnam National University)
Yu, Yong-Man (Dept. Applied Biology, Chungnam National University)
Youn, Young-Nam (Dept. Applied Biology, Chungnam National University)
Publication Information
Korean Journal of Agricultural Science / v.39, no.1, 2012 , pp. 23-28 More about this Journal
Abstract
In order to cloning of PPO gene as a melanin formation related genes involved in hardening and pigmentation of insect integument or wing, we cloned cDNA and analyzed the sequence of PPO gene of H. axyridis. PPO2 primer were designed based on the sequences of PPO genes of Tribolium castaneum and Drosophila melanogaster, and then plasmid DNA were cloned from PCR products obtained from different two color patterns. When the plasmid DNA band pattern were digested by restriction enzymes, BamH1, Xba1, and EcoR1, we found same size band pattern. However, this sequence was not homologous to sequence of T. castaneum PPO gene. Using the primer designed based on the sequence of D. melanogaster, 209 bp PCR product was observed.
Keywords
Harmonia axyridis; PPO (prophenoloxidase); Color polymorphism;
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