Browse > Article
http://dx.doi.org/10.7744/cnujas.2011.38.4.689

Effect of the single nucleotide polymorphism from cytochrome P450 (CYP2A6) gene to fatty acid composition traits in Korean native pig crossed progeny  

Roh, Jung-Gun (Department of Animal Science, Chungbuk National University)
Kim, Sang-Wook (Department of Animal Science, Chungbuk National University)
Kim, Kwan-Suk (Department of Animal Science, Chungbuk National University)
Publication Information
Korean Journal of Agricultural Science / v.38, no.4, 2011 , pp. 689-693 More about this Journal
Abstract
The purpose of this study was to investigate the Cytochrome P450 (CYP2A6) gene as a candidate gene for the traits related with meat fatty acid composition traits in pigs. Porcine CYP2A6 polymorphisms were detected and PCR-RFLP was performed for genotyping of Korean native pig (n=14), Landrace (n=3), Duroc (n=3), Berkshire (n=3), Yorkshire (n=8) and F2 population composed of 202 individuals from an intercross between Korean Native pig and Yorkshire. PCR primer set amplified a 612 bp fragment of CYP2A6 and digestion of the PCR products was performed with the restriction enzymes SchI. The CYP2A6 SchI polymorphism was only found in the KNP breed. The genotype frequencies of TT, TC and CC genotypes were 0.36, 0.56 and 0.08 in the KNP respectively and the other pig breeds were fixed with CC genotype (Duroc, Landrace, Berkshire and Yorkshire). Statistical association between genotypes and fatty acid composition traits were tested in the Korean native pig and Yorkshire crossed F2 pigs. The CYP2A6 SchI polymorphism was associated with only fatty acid composition C20:3n3 level (cis11,14,17-Eicosatrienoic acid, p=0.0252). The 'T' allele was associated with lower C20:3n3 level. Further study is required to validate the genotypic association and biological consequence of the CYP2A6 gene polymorphism in pigs.
Keywords
CYP2A6 gene; Unsaturated Fatty-Acid Composition; Single nucleotide polymorphism;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Ayajiki KH, Fujioka N, Toda S, Okada Y, Minamiyama S, Imaoka, Y. Funae S, Watanabe A, Okamura T. 2003. Mediation of arachidonic acid metabolite(s) produced by endothelial Cytochrome P-450 3A4 in monkey arterial relaxation. Hypertens. Res. 26: 237-243.   DOI   ScienceOn
2 Chen GR, Cue K, Lundstrom JD, Doran O. 2008. Regulation of CYP2A6 Protein Expression by Skatole , Indole, and Testicular Steroids in Primary Cultured Pig. Hepatocytes. Drug. Metab. Dispos. 36: 56-60.
3 Choi KM, Moon JK, Choi SH, Kim KS, Choi YI, Kim JJ, Lee CK. 2008. Differental Expression of Cytochrome P450 Genes Regulate the Level of Adipose Arachidonic Acid Sus Scrofa. Asian-Aust. J. Anim. Sci. 21: 967-971.
4 Chuang SS, Helving C, Taimi M, Ramshaw HA, Collop AH, Amad M, White JA, Petkovich M, Jones G, and Korczak B. 2004. CYP2UI, a novel human thymus- and brain-specific Cytochrome P450, catalyzes omega- and (omega-1)-hydroxylation of fatty acids. J. Biol. Chem. 279: 6305-6314.
5 Kim TH, Choi BH, Chang GW, Lee KT, Lee HY, Lee JH, Kim KS, Park CK, Moran C. 2005. Molecular characterization and chromosomal mapping of porcine adipose differentiation related protein (ADRP). J. Anim. Breed. Genet. 122: 240-246.   DOI   ScienceOn
6 Kim EH, Choi BH, Kim KS, Lee CK, Cho BW, Kim TH, Kim JJ. 2007. Detection of medelian and parent-of-origin quantitative trait loci in a cross between Korean native pig and Landrace I. Growth and body composition traits. Asian-Aust. J. Anim. Sci. 20: 669-676.
7 Lee HA, Yoo IJ, Lee BI. 1997. Research and development trends on omega-3 fatty acid fortified foodstuffs. Korean J. Food. Sci. 26: 161-174. [in Korean]