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http://dx.doi.org/10.7744/cnujas.2011.38.1.065

Analysis of brain protein expression in developing mouse fetus  

Han, Rong-Xun (Dept. of Animal Science & Biotechnology, Chungnam National University)
Kim, Hong-Rye (Dept. of Animal Science & Biotechnology, Chungnam National University)
Diao, Yun-Fei (Dept. of Animal Science & Biotechnology, Chungnam National University)
Woo, Je-Seok (National Institute of Animal Science)
Jin, Dong-Il (Dept. of Animal Science & Biotechnology, Chungnam National University)
Publication Information
Korean Journal of Agricultural Science / v.38, no.1, 2011 , pp. 65-70 More about this Journal
Abstract
Development of mouse fetus brains can be defined morphologically and functionally by three developmental stages, embryo day (ED) 16, postnatal stage one week and eight weeks. These defined stages of brain development may be closely associated with differential gene expression rates due to limited cellular resources such as energy, space, and free water. Complex patterns of expressed genes and proteins during brain development suggests the changes in relative concentrations of proteins rather than the increase in numbers of new gene products. This study was designed to evaluate early protein expression pattern in mouse fetus brain. The mouse brain proteome of fetus at ED 15.5, and 19.5 was obtained using 2-dimensional gel electrophoresis (DE). Analysis of the 2-DE gels in pH 3-10 range revealed the presence of 15 differentially expressed spots, of which 11 spots were identified to be known proteins following MALDI-TOF analysis; 3 spots were up-regulated and 8 spots were down-regulated in the mouse fetus brain at ED 15.5. UP-regulated proteins were identified as MCG18238, isoform M2 of pyruvate kinase isozymes M1/M2, isoform 2 of heterogeneous nuclear ribonucleoprotein K, heterogeneous nuclear ribonucleoprotein H2, creatine kinase B-type, 40S ribosomal protein SA and hemoglobin subunit beta-H1. Down-regulated proteins were putative uncharacterized protein, lactoylglutathione lyase and secreted acidic cysteine rich glycoprotein. Our results revealed composite profiles of mouse fetus brain proteins related to mouse fetus development by 2-DE analysis implying possible roles of these proteins in neural differentiation.
Keywords
Mouse fetus; Brain proteins; 2-DE;
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1 Bradshaw AD, Graves DC, Motamed K, Sage EH. 2003. SPARC-null mice exhibit increased adiposity without significant differences in overall body weight. Proc. Natl. Acad. Sci. 100: 6045-6050.
2 Gorg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, Weiss W. 2000. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 21: 1037-1053.   DOI   ScienceOn
3 Gotz, M, Huttner WB. 2005. The cell biology of neurogenesis. Nat. Rev. Mol. Cell Biol. 6: 777-788.   DOI   ScienceOn
4 Greenberg DA, Jin K. 2006. Neurodegeneration and neurogenesis: Focus on Alzheimer's disease. Curr. Alzheimer Res. 3: 25-28.   DOI   ScienceOn
5 Guillemot F. 2007. Cell fate specification in the mammalian telencephalon. Prog. Neurobiol. 134: 3771-3780.
6 Hartl D, Irmler M, Romer I, Mader MT, Mao L, Zabel C, de Angelis MH, Beckers J, Klose J. 2008. Transcriptome and proteome analysis of early embryonic mouse brain development. Proteomics 8: 1257-1265.   DOI   ScienceOn
7 Hirabayashi Y, Gotoh Y. 2005. Stage-dependent fate determination of neural precursor cells in mouse forebrain. Neurosci. Res. 51: 331-336.   DOI   ScienceOn
8 Ikeda Y, Noguchi T. 1998. Allosteric regulation of pyruvate kinase M-2 isozyme involves a cysteine residue in the intersubunit contact. J. Biol. Chem. 273: 12227-12233.   DOI
9 Kagami Y, Furuichi T. 2001. Investigation of differentially expressed genes during the development of mouse cerebellum. Brain Res. Gene Expr. Patterns 1: 39-59.   DOI
10 Kaindl AM, Sifringer M, Zabel C, Nebrich G, Wacker MA, Felderhoff-Mueser U, Endesfelder S, Hagen M, Stefovska V, Klose J, Ikonomidou C. 2006. Acute and long-term proteome changes induced by oxidative stress in the developing brain. Cell Death Differ. 13: 1097-1099.   DOI   ScienceOn
11 Matsuki T, Hori G, Fruichi T. 2005. Gene expression profiling during the embryonic development of mouse brain using an oligonucleotide-based microarray system. Brain Res. Mol. Brain Res. 136: 231-254.   DOI
12 Qian X, Shen Q, Goderie SK, He W, Capela A, Davis AA, Temple S. 2000. Timing of CNS cell generation: A programmed sequence of neuron and glial cell production from isolated murine cortical stem cells. Neuron 28: 69-80.   DOI   ScienceOn
13 Seefeldt I, Nebrich G, Römer I, Mao L, Klose J. 2006. Evaluation of 2-DE protein patterns from pre- and postnatal stages of the mouse brain. Proteomics 6: 4932-4939.   DOI   ScienceOn
14 Shevchenko A, Wilm M, Vorm O, Mann M. 1996. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 68: 850-858.   DOI   ScienceOn