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http://dx.doi.org/10.7744/cnujas.2011.38.1.051

Analyses of single nucleotide polymorphisms and haplotypes of BoLA-DRB3 gene in Holstein and Hanwoo  

Jeong, Hang-Jin (Dept. of Animal Biosystem Science, Chungnam National University)
Yu, Seong-Lan (Dept. of Animal Science and Biotechnology, Chungnam National University)
Hoque, M.R. (Dept. of Animal Science and Biotechnology, Chungnam National University)
Lee, Jun-Heon (Dept. of Animal Science and Biotechnology, Chungnam National University)
Do, Chang-Hee (Dept. of Animal Biosystem Science, Chungnam National University)
Ryoo, Seung-Heui (Government of Changcheongnam-Do, Livestock Research Institute)
Sang, Byung-Chan (Dept. of Animal Biosystem Science, Chungnam National University)
Publication Information
Korean Journal of Agricultural Science / v.38, no.1, 2011 , pp. 51-63 More about this Journal
Abstract
BoLA (bovine leukocyte antigens) have been known as gene complex related with bovine diseases and immunological traits. This study was conducted to find out the characteristics of BoLA-DRB3 gene related to mastitis and BL(bovine leukocyte) from 280 cattle [193 animals of Holstein cattle and 87 animals of Hanwoo]. As a result, five PCR-RFLP types (b, d, e, f and g) using HaeIII restriction enzyme, three BstYI restriction patterns (b, d and e) and eight RsaI restriction types(b, d, f, I, j, n, o and w) were identified. Moreover, we identified new d' type ($197{\rightarrow}175$/22), having one more cutting site by BstYI enzyme than d type allele and n' type ($180{\rightarrow}169$/11) having one more cutting site by RsaI enzyme than n allele was additionally identified. Next, we identified 53 SNPs in BoLA-DRB3 exon2 from 280 cattle. SNP frequency and heterozygosity of Holstein and Hanwoo were investigated in all the SNP genotype. These results might be based on research for identifying marker associated with bovine diseases.
Keywords
BoLA-DRB3; PCR-RFLP; Holstein; Hanwoo;
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