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http://dx.doi.org/10.7744/cnujas.2010.37.3.367

Molecular Cloning and Characterization of cDNA Encoding Farnesyl Diphosphate Synthase in Garlic (Allium sativum)  

Pham, Anh Tuan (Department of Crop Science, Chungnam National University)
Park, Nam-Il (Department of Crop Science, Chungnam National University)
Kim, Haeng-Hoon (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration (RDA))
Park, Sang-Un (Department of Crop Science, Chungnam National University)
Publication Information
Korean Journal of Agricultural Science / v.37, no.3, 2010 , pp. 367-371 More about this Journal
Abstract
Farnesyl diphosphate synthase (FPS) catalyzes the biosynthesis of farnesyl diphosphate, a precursor for many important terpenoid products. A cDNA encoding FPS was first isolated from Allium sativum (AsFPS) using rapid amplification of cDNA ends (RACE) PCR. The sequence of AsFPS contains an open reading frame encoding a protein of 341 amino acids with a predicted molecular mass of 39.61 kDa. Alignment of AsFPS deduced amino acid revealed high identities with other plants ranging from 79% to 85% and showed 2-high conserved aspartate-rich motifs known to be important for FPS activity. Furthermore, AsFPS expression was stronger in the green organs such as bulbils, scapes, leaves, stems, but weaker in bulbs and roots than on-green organs of A. sativum.
Keywords
Allium sativum; Farnesyl diphosphate synthase; Gene expression;
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