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http://dx.doi.org/10.4041/kjod.2013.43.6.294

Role of interleukin-6 in orthodontically induced inflammatory root resorption in humans  

Kunii, Ryuichi (Department of Orthodontics, Nihon University School of Dentistry at Matsudo)
Yamaguchi, Masaru (Department of Orthodontics, Nihon University School of Dentistry at Matsudo)
Tanimoto, Yasuhiro (Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo)
Asano, Masaki (Department of Orthodontics, Nihon University School of Dentistry at Matsudo)
Yamada, Kunihiko (Department of Orthodontics, Nihon University School of Dentistry at Matsudo)
Goseki, Takemi (Department of Orthodontics, Nihon University School of Dentistry at Matsudo)
Kasai, Kazutaka (Department of Orthodontics, Nihon University School of Dentistry at Matsudo)
Publication Information
The korean journal of orthodontics / v.43, no.6, 2013 , pp. 294-301 More about this Journal
Abstract
Objective: To determine the interleukin (IL)-6 levels in gingival crevicular fluid (GCF) of patients with severe root resorption after orthodontic treatment and investigate the effects of different static compressive forces (CFs) on IL-6 production by human periodontal ligament (hPDL) cells and the influence of IL-6 on osteoclastic activation from human osteoclastic precursor (hOCP) cells in vitro. Methods: IL-6 levels in GCF samples collected from 20 patients (15 and 5 subjects without and with radiographic evidence of severe root resorption, respectively) who had undergone orthodontic treatment were measured by ELISA. The levels of IL-6 mRNA in hPDL cells and IL-6 protein in conditioned medium after the application of different uniform CFs (0, 1.0, 2.0, or 4.0 $g/cm^2$ for up to 72 h) were measured by real-time PCR and ELISA, respectively. Finally, the influence of IL-6 on mature osteoclasts was investigated by using hOCP cells on dentin slices in a pit-formation assay. Results: Clinically, the IL-6 levels were significantly higher in the resorption group than in the control group. In vitro, IL-6 mRNA expression significantly increased with increasing CF. IL-6 protein secretion also increased in a time- and magnitude-dependent manner. Resorbed areas on dentin slices were significantly greater in the recombinant human IL-6-treated group and group cultured in hPDL cell-conditioned medium with CF application (4.0 $g/cm^2$) than in the group cultured in hPDL cell-conditioned medium without CF application. Conclusions: IL-6 may play an important role in inducing or facilitating orthodontically induced inflammatory root resorption.
Keywords
Interleukin-6; Osteo/odontoclastogenesis; Orthodontic force; Root resorption;
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