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http://dx.doi.org/10.4041/kjod.2012.42.6.307

Differentiation and characteristics of undifferentiated mesenchymal stem cells originating from adult premolar periodontal ligaments  

Kim, Seong Sik (Department of Orthodontics, School of Dentistry, Pusan National University)
Kwon, Dae-Woo (Department of Orthodontics, School of Dentistry, Pusan National University)
Im, Insook (Department of Orthodontics, School of Dentistry, Pusan National University)
Kim, Yong-Deok (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
Hwang, Dae-Seok (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
Holliday, L. Shannon (Department of Orthodontics, School of Dentistry, University of Florida)
Donatelli, Richard E. (Department of Orthodontics, School of Dentistry, University of Florida)
Son, Woo-Sung (Department of Orthodontics, School of Dentistry, Pusan National University)
Jun, Eun-Sook (Biomedical Research Institute, Pusan National University Hospital)
Publication Information
The korean journal of orthodontics / v.42, no.6, 2012 , pp. 307-317 More about this Journal
Abstract
Objective: The purpose of this study was to investigate the isolation and characterization of multipotent human periodontal ligament (PDL) stem cells and to assess their ability to differentiate into bone, cartilage, and adipose tissue. Methods: PDL stem cells were isolated from 7 extracted human premolar teeth. Human PDL cells were expanded in culture, stained using anti-CD29, -CD34, -CD44, and -STRO-1 antibodies, and sorted by fluorescent activated cell sorting (FACS). Gingival fibroblasts (GFs) served as a positive control. PDL stem cells and GFs were cultured using standard conditions conducive for osteogenic, chondrogenic, or adipogenic differentiation. Results: An average of $152.8{\pm}27.6$ colony-forming units was present at day 7 in cultures of PDL stem cells. At day 4, PDL stem cells exhibited a significant increase in proliferation (p < 0.05), reaching nearly double the proliferation rate of GFs. About $5.6{\pm}4.5%$ of cells in human PDL tissues were strongly STRO-1-positive. In osteogenic cultures, calcium nodules were observed by day 21 in PDL stem cells, which showed more intense calcium staining than GF cultures. In adipogenic cultures, both cell populations showed positive Oil Red O staining by day 21. Additionally, in chondrogenic cultures, PDL stem cells expressed collagen type II by day 21. Conclusions: The PDL contains multipotent stem cells that have the potential to differentiate into osteoblasts, chondrocytes, and adipocytes. This adult PDL stem cell population can be utilized as potential sources of PDL in tissue engineering applications.
Keywords
Histochemistry; Periodontics; Bone biology;
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1 Hou LT, Li TI, Liu CM, Liu BY, Liu CL, Mi HW. Modulation of osteogenic potential by recombinant human bone morphogenic protein-2 in human periodontal ligament cells: effect of serum, culture medium, and osteoinductive medium. J Periodontal Res 2007;42:244-52.   DOI   ScienceOn
2 Yamagiwa H, Endo N, Tokunaga K, Hayami T, Hatano H, Takahashi HE. In vivo bone-forming capacity of human bone marrow-derived stromal cells is stimulated by recombinant human bone morphogenetic protein-2. J Bone Miner Metab 2001;19:20-8.   DOI   ScienceOn
3 Lecka-Czernik B, Moerman EJ, Grant DF, Lehmann JM, Manolagas SC, Jilka RL. Divergent effects of selective peroxisome proliferator-activated receptorgamma 2 ligands on adipocyte versus osteoblast differentiation. Endocrinology 2002;143:2376-84.   DOI   ScienceOn
4 Krishnan V, Davidovitch Z. Cellular, molecular, and tissue-level reactions to orthodontic force. Am J Orthod Dentofacial Orthop 2006;129:469.e1-32.
5 Mao JJ, Giannobile WV, Helms JA, Hollister SJ, Krebsbach PH, Longaker MT, et al. Craniofacial tissue engineering by stem cells. J Dent Res 2006;85:966-79.   DOI   ScienceOn
6 Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs)in vitro and in vivo. Proc Natl Acad Sci U S A 2000; 97:13625-30.   DOI   ScienceOn
7 Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004;364:149-55.   DOI   ScienceOn
8 Gay IC, Chen S, MacDougall M. Isolation and characterization of multipotent human periodontal ligament stem cells. Orthod Craniofac Res 2007;10:149-60.   DOI   ScienceOn
9 Shi S, Bartold PM, Miura M, Seo BM, Robey PG, Gronthos S. The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthod Craniofac Res 2005;8:191-9.   DOI   ScienceOn
10 Jo YY, Lee HJ, Kook SY, Choung HW, Park JY, Chung JH, et al. Isolation and characterization of postnatal stem cells from human dental tissues. Tissue Eng 2007;13:767-73.   DOI   ScienceOn
11 Laino G, d'Aquino R, Graziano A, Lanza V, Carinci F, Naro F, et al. A new population of human adult dental pulp stem cells: a useful source of living autologous fibrous bone tissue (LAB). J Bone Miner Res 2005;20:1394-402.   DOI   ScienceOn
12 Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG, et al. SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci U S A 2003; 100:5807-12.   DOI   ScienceOn
13 Digirolamo CM, Stokes D, Colter D, Phinney DG, Class R, Prockop DJ. Propagation and senescence of human marrow stromal cells in culture: a simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate. Br J Haematol 1999;107:275-81.   DOI   ScienceOn
14 Reyes M, Lund T, Lenvik T, Aguiar D, Koodie L, Verfaillie CM. Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood 2001;98:2615-25.   DOI   ScienceOn
15 Galotto M, Campanile G, Robino G, Cancedda FD, Bianco P, Cancedda R. Hypertrophic chondrocytes undergo further differentiation to osteoblast-like cells and participate in the initial bone formation in developing chick embryo. J Bone Miner Res 1994;9:1239-49.
16 Bennett JH, Joyner CJ, Triffitt JT, Owen ME. Adipocytic cells cultured from marrow have osteogenic potential. J Cell Sci 1991;99:131-9.
17 Caplan AI. Mesenchymal stem cells. J Orthop Res 1991;9:641-50.   DOI
18 Majumdar MK, Thiede MA, Mosca JD, Moorman M, Gerson SL. Phenotypic and functional comparison of cultures of marrow-derived mesenchymal stem cells (MSCs) and stromal cells. J Cell Physiol 1998;176:57-66.   DOI   ScienceOn
19 Simmons PJ, Torok-Storb B. Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood 1991;78:55-62.
20 Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet 1970;3:393-403.
21 Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143-7.   DOI   ScienceOn
22 Alhadlaq A, Mao JJ. Tissue-engineered osteochondral constructs in the shape of an articular condyle. J Bone Joint Surg Am 2005;87:936-44.   DOI   ScienceOn
23 Dogan A, Ozdemir A, Kubar A, Oygur T. Healing of artificial fenestration defects by seeding of fibroblastlike cells derived from regenerated periodontal ligament in a dog: a preliminary study. Tissue Eng 2003;9:1189-96.   DOI   ScienceOn
24 Nakahara T, Nakamura T, Kobayashi E, Kuremoto K, Matsuno T, Tabata Y, et al. In situ tissue engineering of periodontal tissues by seeding with periodontal ligament-derived cells. Tissue Eng 2004;10:537-44.   DOI   ScienceOn
25 Akizuki T, Oda S, Komaki M, Tsuchioka H, Kawakatsu N, Kikuchi A, et al. Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs. J Periodontal Res 2005;40:245-51.   DOI   ScienceOn
26 Young HE, Duplaa C, Katz R, Thompson T, Hawkins KC, Boev AN, et al. Adult-derived stem cells and their potential for use in tissue repair and molecular medicine. J Cell Mol Med 2005;9:753-69.   DOI   ScienceOn
27 Hasegawa M, Yamato M, Kikuchi A, Okano T, Ishikawa I. Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model. Tissue Eng 2005;11:469-78.   DOI   ScienceOn
28 Nussenbaum B, Rutherford RB, Teknos TN, Dornfeld KJ, Krebsbach PH. Ex vivo gene therapy for skeletal regeneration in cranial defects compromised by postoperative radiotherapy. Hum Gene Ther 2003;14:1107-15.   DOI   ScienceOn
29 Sloan AJ, Smith AJ. Stem cells and the dental pulp: potential roles in dentine regeneration and repair. Oral Dis 2007;13:151-7.   DOI   ScienceOn
30 Kemp KC, Hows J, Donaldson C. Bone marrow-derived mesenchymal stem cells. Leuk Lymphoma 2005; 46:1531-44.   DOI   ScienceOn
31 Sakaguchi Y, Sekiya I, Yagishita K, Muneta T. Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source. Arthritis Rheum 2005;52:2521-9.   DOI   ScienceOn
32 Müssig E, Tomakidi P, Steinberg T. Molecules contributing to the maintenance of periodontal tissues. Their possible association with orthodontic tooth movement. J Orofac Orthop 2005;66:422-33.   DOI   ScienceOn
33 Sawa Y, Phillips A, Hollard J, Yoshida S, Braithwaite MW. The in vitro life-span of human periodontal ligament fibroblasts. Tissue Cell 2000;32:163-70.   DOI   ScienceOn
34 Baddoo M, Hill K, Wilkinson R, Gaupp D, Hughes C, Kopen GC, et al. Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection. J Cell Biochem 2003;89:1235-49.   DOI   ScienceOn
35 Lemoli RM, Tafuri A, Fortuna A, Catani L, Rondelli D, Ratta M, et al. Biological characterization of CD34+ cells mobilized into peripheral blood. Bone Marrow Transplant. 1998;22(Suppl 5):S47-50.   DOI
36 Waller EK, Olweus J, Lund-Johansen F, Huang S, Nguyen M, Guo GR, et al. The "common stem cell" hypothesis reevaluated: human fetal bone marrow contains separate populations of hematopoietic and stromal progenitors. Blood 1995;85:2422-35.