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Expression of UNC-50 DNA in periodontal tissue of rats after application of intermittent orthodontic force  

Park, Mi-Kyoung (Department of Orthodontics, College of Dentistry, Chosun University)
Lim, Sung-Hoon (Department of Orthodontics, College of Dentistry, Chosun University)
Kim, Kwang-Won (Department of Orthodontics, College of Dentistry, Chosun University)
Park, Joo-Cheol (Department of Oral Histology, College of Dentistry, Chosun University)
Publication Information
The korean journal of orthodontics / v.36, no.4, 2006 , pp. 242-250 More about this Journal
Abstract
Objective: Periodontal ligament fibroblasts have an ectomesenchymal origin and are thought to play a crucial role for not only homeostasis of periodontal tissues but also bone remodeling, wound healing and regeneration of tissues. Recently, it has been reported that UNC-50 is not expressed in gingival fibroblasts but in PDL fibroblasts. The purpose of this study was to examine the expression of UNC-50 and osteocalcin in the periodontium after application of intermittent force. Methods: Twelve rats had 40 grams of mesially-directed force applied at the upper molar for 1 hour/day. Four rats were sacrificed at 1, 3 and 5 days. Immunohistochemical localization of UNC-50 and osteocalcin antibody was carried out. The results showed apposition of new cellular cementum and a slight increase in periodontal space at the tension side. Results: Strong UNC-50 expression was observed in the differentiating cementoblasts close to PDL fibroblasts in the tension side whereas it was barely expressed at the compression side. Expression was strong at day 3, and decreased at day 5. Osteocalcin immunoreactivity expression was strong in differentiating cementoblasts at the tension side. Conclusion: It can be suggested that UNC-50 is related to the differentiation of cementoblasts, and may be responsible for the molecular event in PDL cells under mechanical stress.
Keywords
Mechanical stress; UNC-50; Cementoblast;
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  • Reference
1 McCulloch CA. Basic considerations in periodontal wound healing to achieve regeneration. Periodontol 2000 1993;1:16-25
2 Cochran DL, Wozney JM. Biological mediators for periodontal regeneration. Periodontol 1999;19:40-58   DOI   ScienceOn
3 Shimono M, Ishikawa T, Ishikawa H, Matsuzaki H, Hashimoto S, Muramatsu T, Shima K, Matsuzaka K, Inoue T. Regulatory mechanisms of periodontal regeneration. Microsc Res Tech 2003;60:491-502   DOI   ScienceOn
4 Kim HJ, Jeong MJ, Kim BO, Kook JK, Kim CK, Park JC. Expression of periodontal ligament fibroblast-specific gene, PDLs22 during development and differentiation of periodontal tissue J Kor Oral Maxillofac Pathol 2005;29:17-23
5 Dennison DK, Vallone DR, Pinero GJ, Rittman B, Caffesse RG. Differential effect of $TGF-{\beta}1$ and PDGF on proliferation of periodontal ligament cells and gingival fibroblasts. J Periodontol 1994;65:641-8   DOI   ScienceOn
6 Nohutcu RM, Somerman MJ, McCauley LK. Dexamethasone enhances the effects of parathyroid hormone on human periodontal ligament cells in vitro. Calcif Tissue Int 1995;56:571-7   DOI
7 Ruch JV. Odontoblast commitment and differentiation. Biochem Cell Biol 1998;76:923-38   DOI
8 King GJ, Keeling SD, McCoy EA, Ward TH. Measuring dental drift and orthodontic tooth movement in response to various initial forces in adult rats. Am J Orthod Dentofacial Orthop 1991;99:456-65   DOI   ScienceOn
9 Reitan K. Tissue behavior during orthodontic movement. Am J Orthod 1960;46:881-900   DOI   ScienceOn
10 Fitzgerald J, Kennedy D, Viseshakul N, Cohen BN, Mattick J, Bateman JF, Forsayeth JR. UNCL, the mammalian homologue of UNC-50, is an inner nuclear membrane RNA-binding protein. Brain Res 2000;877:110-23   DOI   ScienceOn
11 Gibson JM, King GJ, Keeling SD. Long-term orthodontic tooth movement response to short-term force in the rat. Angle Orthod 1992;62:211-5
12 Canalis E, McCarthy T, Centrella M. Effects of basic fibroblast growth factor on bone formation in vitro. J Clin Invest 1988;81:1572-77   DOI   ScienceOn
13 Schroeder HE. The periodontium. Berlin: Springer-Verlag;1986
14 Cho MI, Garant PR. Expression and role of epidermal growth factor receptors during differentiation of cementoblasts, osteoblasts, and periodontal ligament fibroblasts in the rat. Anat Rec 1996;245:342-60   DOI   ScienceOn
15 Reddi AH, Cunningham NS. Initiation and promotion of bone differentiation by bone morphogenetic proteins. J Bone Miner Res 1993;8(2 suppl):499S-502S   DOI
16 O'Connor JA, Lanyon LE, MacFie H. The influence of strain rate on adaptive bone remodelling. J Biomech 1982;15:767-81   DOI   ScienceOn
17 Davidovitch Z, Shanfeld JL. Cyclic AMP levels in alveolar bone of orthodontically-treated cats. Arch Oral Biol 1975;20:567-74   DOI   ScienceOn
18 Hammarstrom L, Heijl L, Gestrelius S. Periodontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins. J Clin Periodontol 1997;24:669-77   DOI   ScienceOn
19 Kernan M, Cowan D, Zuker C. Genetic dissection of mechanosensory transduction : mechanoreception-defective mutations of Drosophila. Neuron 1994;12:1195-206   DOI   ScienceOn
20 Reitan K. Some factors determining the evaluation of forces in orthodontics. Am J Orthod 1957;43:32-45   DOI   ScienceOn
21 Nilda A, John JS, Jennifer M, Ruth AF, Martha J. Human periodontal cells initiate mineral-like nodule in vitro. J periodontal 1991;62:499-503   DOI
22 Ramakrishnan PR, Lin WL, Sodek J, Cho MI. Synthesis of noncollagenous extracellualar matrix proteins during development of mineralized nodules by rat periodontal ligament cells in vitro. Calcif Tissue Int 1995;57:52-9   DOI
23 Chien HH, Lin WL, Cho MI. Expression of TGF-beta isoforms and their receptors during mineralized nodule formation by rat periodontal ligament cells in vitro. J Periodontal Res 1999;34:301-9   DOI
24 Kwon SY, Bae SM, Kyung HM, Sung JH. The effect of continuous and intermittent compressive pressure on alkaline phosphatase activity of periodontal ligament cells. Koean J Orthod 1997;27:599-605   과학기술학회마을
25 Kirino Y, Tsuchiya T, Kurihara S, Chiba M, Miura F. A study of tooth movement with super-elastic force. J Jpn Orthod Soc 1991;99: 315-24
26 Park JC, Kim YB, Kim HJ, Jang HS, Kim HS, Kim BO, Han KY. Isolation and charaterization of cultured human periodental ligament fibroblast-specific cDNAs. Biochem Biophys Res Commun 2001; 282:1145-53   DOI   ScienceOn
27 Perrimon N, Engstrom L, Mahowald AP. Zygotic lethals with specific maternal effect phenotypes in Drosophila melanogaster. I. Loci on the X chromosome, Genetics 1989;121:333-52
28 Cho MI, Matsuda N, Lin WL, Moshier A, Ramakrishnan PR. In vitro formation of mineralized nodules by periodontal ligament cells from the rat. Calcif Tissue Int 1992;50:459-67   DOI
29 Oppenheim A. A possibility for physiologic orthodontic movement. Am J Orthod Oral Surg 1944;30:277-328   DOI   ScienceOn
30 Howes R, Bowness JM, Grotendorst GR, Martin GR, Reddi AH. Platelet-derived growth factor enhances demineralized bone matrix-induced cartilage and bone formation. Calcif Tissue Int 1998;42:34-8   DOI
31 Cho MI, Lin WL, Garant PR. Occurrence of epidermal growth factor-binding sites during differentiation of cementoblasts and periodontal ligament fibroblasts of the young rat: a light and electron microscopic radioautographic study. Anat Rec 1991;231:14-24   DOI   ScienceOn
32 Konoo T, Kim YJ, Gu GM, King GJ. Intermittent force in orthodontic tooth movement. J Dent Res 2001;80:457-60   DOI   ScienceOn
33 Beertsen W, McCulloch CA, Sodek J. The periodontal ligament: a unique, multifunctional connective tissue. Periodontol 2000 1997;13: 20-40   DOI   ScienceOn
34 Ji S, Kim BO, Kim SM, Park CJ. Expression of peridontal ligament fibroblast-specific gene, PDLs22 during development of periodontal ligament, alveolar bone and cementum. Korean J Anat 2003;36: 123-32
35 Nancy A, Ten Cate's Oral Histology : Development, structure, and function. 6th ed. St Louis: Mosby; 2003. p. 240-274
36 Perrimon N, Smouse D, Miklos GL. Developmental genetics of loci at the base of the X chromosome of Drosophila melanogaster. Genetics 1989;121:313-31