Browse > Article

Effects on Osteoclast in Periodontal Ligament Space by Denerveation of Inferior Alveolar Nerve in Young and Adult Rats  

Park, Kyung-Duk (Mi Dental Clinic)
Sung, Jae-Hyun (Sung Jae Hyun Dental Clinic)
Bae, Yong-Chul (Department of Oral Anatomy, College of Dentistry, Kyungpook National University)
Kyung, Hee-Moon (Department of Orthodontics, College of Dentistry, Kyungpook National University)
Publication Information
The korean journal of orthodontics / v.34, no.6, 2004 , pp. 506-513 More about this Journal
Abstract
Osteoclast action is necessary for alveolar bone remodeling in orthodontic tooth movement. The nervous system has also been reported to be associated with bone remodeling. This study was aimed to investigate the changes of osteoclasts in the periodontal ligament (PDL) space after surgical resection of the inferior alveolar nerve (IAN). Experimental rats were divided into young and adult groups. A surgical resection procedure of the IAN was carried out in the left side of the mandible and a sham operation in the right side of the mandible. The number of osteoclasts on the bundle bone surface and the resorption activity of the osteoclasts were histomorphometrically measured. The changes in distribution of substance P (SP) immunoreactive (IR) nerve fiber were evaluated in the PDL and pulp. SP-IR nerve fiber was depleted in both the PDL and pulp of the IAN resection side in both groups, which confirmed the resection of IAN to be successfully conducted. The number of osteoclasts in the IAN resection side was significantly reduced in both the young and adult groups (p<0.01 and p<0.05), whereas the resorption activity of osteoclasts did not show any significant difference between the IAN resection side and the sham operation side in both groups (p>0.05 and p<0.05). The adult group showed that the number of osteoclasts reduced significantly (p<0.01) and the resorption activity didn't change in comparison with the young group (p>0.05). These results suggest that surgical resection of the IAN and aging reduce the population of the recruited osteoclasts within the PDL, but don't affect on the osteoclastic resorption activity.
Keywords
Osteoclast; Inferior alveolar nerve; Denervation; Periodontal ligament space;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Silverman JD, Kruger L. An interpretation of dental innervation based upon the pattern of calcitonin gene-related peptide (CGRP)-immunoreactive thin sensory axons. Somatosensory Research 1987;5:157-75   DOI   PUBMED
2 Wakisaka S. Lchikawa H, Nishikawa S, Matsuo S, Takano Y, Akai M.The distribution andorigin of calcitonin gene-related peptidecontaining nerve fibers In feline dental pulp Relationship With substance p-containing norve fibres. Histochemistry 1987:86: 585-9
3 Hill EL, Turner R, Elde R. Effects of neonatal syrnphathectorny and capsaicin treatment on bone remodeling in rats. Neuroscience 1991 :44:747-55
4 D'Souza SM, Macintyre I, Girgis SI, Mundy GR. Human synthetic calcitonin gene-related peptide inhibits bone resorption in vitro. Endocrinology 1986;19:58-61
5 Gulbenkian S, Merighi A, Wharton J, Vamdell IM, Polak JM. Ultrastructural evidence for coexistence of calcitonin gene-related peptide and substance P in secretory vesicles of peripheral nerve in theguinea pig.J Neurocytol 1986;15:535-42   DOI   PUBMED
6 Roodman GD. Cell biology of the osteoclast. Exp Hematol 1999: 27:1229-41.
7 Lerner UH. Osteoclast formation and resorption. Matrix Biology 2000;19:107-20   DOI   ScienceOn
8 Mori T, Ogata T, Okumuram H, Shibata T, Nakamura Y, Kataoka K. Substance P regulates the function of rabbit cultured osteoclast; increase of intracellular free calcium concentration and enhancement of bone resorption. Biochem Biophy Res Commun 1999;262:418-22   DOI   ScienceOn
9 Berger RL, Byers MR, Calkins DF. Dental nerve regeneration in rats I. Electrophysiological studies of molar sensory deficit and recovery. Pain 1983:15:345-57
10 Hokfelf T, Kellerth JO, Nilsson G, Pemow B. Experimental immunohistochemical studies on the localization and distribution of substance P in cat primary sensory neurons. Brain Research 1975;100:235-52   DOI   ScienceOn
11 Suda T, Takahashi N, Martin TJ. Modulation of osteoclast diff-erentiation. Endocrine Reviews 1992:13:66-80
12 Wakisaka S. Neuropeptides in thedental pulp: distribution, Origins, andcorrelation. J Endod 1990:16:67-9
13 Zaidi M, Pazianas M, Shankar V, Bax B, Bax C, Belvis P, Stevens C, Huang C, Blake D, Moonga BS, Alam A. Osteoclast function anditscontrol. Experimental Physiology 1993:78:721-39
14 Vignery A, McCarthy TL. The neuropeptide calcitonin gene-related peptide stimulates insulin-like growth factor I production by primary fetal ratosteoblasts. Bone 1996;18:331-5   DOI   ScienceOn
15 Euler VUS, Gaddum JH. An unidentifed depressor substance in certain tissue extracts. J Physiol(Lond.) 1931 ;72:74-87
16 Adam C, Llorens A, Baroukh B, Cherruau M,Saffar JL. Effect of capsaicin-induced sensory denervation on osteoclastic resorption in adult rats. Exp Physiol 2000;85:61-6   DOI   ScienceOn
17 Rameshwar P, Ganea D, Gascon P. In vitro stimulatory effect of substance Pon hematopoiesis. Blood 1993;81 :391-8   PUBMED
18 Goto T, Yamaza T, Kido MA, Tanaka T. Light-and electronmicorscopic study of the distribution ofaxons containing substance P and the localization of neurokinin-1 receptor in bone. Cell Tissue Res 1998;293:87-9   DOI
19 Zaidi M, Fuller K, Bevis PJ, GaineseDas RE, Chambers TJ, Macintyre I. Calcitonin gene-related peptide inhibits osteoclastic bone resorption: a comparative study. Calcified Tissue Intemational 1987: 40:149-54
20 Robinson PP. Reinnervation of teeth, mucous membrane andskin following section of theinferior alveolar nerve in thecat. Brain Res 1981 ;220:241-53   DOI   ScienceOn
21 Yamashiro T, Fujiyama Y, Fujiyoshi N, Inaguma, TakanoYamamoto T. Inferior alveolar nerve transection inhibits increase in osteoclast appearance during experimental tooth movement. Bone 2000;26:663-9.   DOI   ScienceOn
22 Wakisaka S, Nishikawa S, Ichikawa H, Matsuo S, Takano Y, Akai M.The distribution andorigin of substance Plike immunoreactivity in the rat molar pulpand periodontal tissues. Arch Oral Biol 1985; 30: 813-8   DOI   ScienceOn
23 Adamus MA, Dabrowski ZJ. Effect of theneuropeptide substance Pon therat bonemarrow-derived osteogenic cells in vitro. J cell Biochem 2001 ;81 :499-506   DOI   ScienceOn
24 Vandevska-Radunovic V, Hals kvinnsland I, Kvinnsland S. Effect of inferior alveolar nerve axotomy on periodontal andpulpal blood flow subsequent to experimental tooth movement in rats. Acta Odontol Scand 1998:56:57-64