Browse > Article
http://dx.doi.org/10.13065/jksdh.20210053

Quantitative detection of peri-implantitis bacteria using real-time PCR  

Kim, Min-Jung (Department of Dental Hygiene, Graduate School of Gachon University)
Han, Gyeong-Soon (Department of Dental Hygiene, Graduate School of Gachon University)
Publication Information
Journal of Korean society of Dental Hygiene / v.21, no.5, 2021 , pp. 555-565 More about this Journal
Abstract
Objectives: This study was conducted to analyze peri-implantitis bacteria and identify their associations with health status and health activities. Methods: Gingival sulcus fluid at the implant's periodontal pockets sampled from the participants were analyzed by multiplex real time PCR. Results: Participants had strains in the order of 100% F. nucleatum, 98.0% E. corrodens, and 96.0% P. micra, and the correlation between C. rectus and E. nodatum was high (p<0.01). Diabetic group (P. gingivalis, P. nigrescens) hypertension (P. nigrescens), group with four or more periodontal pockets (P. gingivalis, T. dentica, P. intermedia, E. nodatum, and C. rectum), smoking (P. micra, E. corrodens), drinking (T. dentola), and scaling groups (C. rectus) were found to have more strains (p<0.05). Conclusions: Representative pathogenic microorganisms detected in periodontal pockets of implants were similar to dental periodontal pockets; however there were differences in the amount and distribution of microorganisms, and they were affected by health status and health behavior.
Keywords
Multiplex real-time PCR; Oral pathogenic microorganisms; Peri-implantitis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Galindo-Moreno P, Fauri M, Avila-Ortiz G, Fernandez-Barbero JE, Cabrera-Leon A, Sanchez-Fernandez E. Influence of alcohol and tobacco habits on peri-implant marginal bone loss: a prospective study. Clin Oral Implants Res 2005;16(5):579-86. https://doi.org/10.1111/j.1600-0501.2005.01148.x   DOI
2 Serino G, Strom C. Peri-implantitis in partially edentulous patients: association with inadequate plaque control. Clin Oral Implants Res 2009;20(2):169-74. https://doi.org/10.1111/j.1600-0501.2008.01627.x   DOI
3 Kumar PS, Mason MR, Brooker MR, O'Brien K. Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants. J Clin Periodontol 2012;39(5):425-33. https://doi.org/10.1111/j.1600-051X.2012.01856.x   DOI
4 Sakamoto M, Takeuchi Y, Umeda M, Ishikawa I, Benno Y. Rapid detection and quantification of five periodontopathic bacteria by real-time PCR. Microbiol Immunol 2001;45(1):39-44. https://doi.org/10.1111/j.1348-0421.2001.tb01272.x   DOI
5 Froum SJ, Rosen PS. A proposed classification for peri-implantitis. Int J Periodontics Restorative Dent 2012;32(5):533-40.
6 Haffajee AD, Socransky SS, Patel MR, Song X. Microbial complexes in supragingival plaque. Oral Microbiol Immunol 2008;23(3):196-205. https://doi.org/10.1111/j.1399-302X.2007.00411.x   DOI
7 Da Silva-Boghossian CM, do Souto RM, Luiz RR, Colombo AP. Association of red complex, A. actinomycetemcomitans and non-oral bacteria with periodontal diseases. Arch Oral Biol 2011;56(9):899-906. https://doi.org/10.1016/j.archoralbio.2011.02.009   DOI
8 Nguyen-Hieu T, Borghetti A, Aboudharam G. Peri-implantitis: from diagnosis to therapeutics. J Investig Clin Dent 2012;3(2):79-94. https://doi.org/10.1111/j.2041-1626.2012.00116.x   DOI
9 Ebersole JL, Holt SC, Hansard R, Novak MJ. Microbiologic and immunologic characteristics of periodontal disease in Hispanic americans with type 2 diabetes. J Periodontol 2008;79(4):637-46. https://doi.org/10.1902/jop.2008.070455   DOI
10 Moy PK, Medina D, Shetty V, Aghaloo TL. Dental implant failure rates and associated risk factors. Int J Oral Maxillofac Implants 2005;20(4):569-77.
11 Sastrowijoto SH, Hillemans P, Van Steenbergen TJ, Abraham-Inpijn L, De Graaff J. Periodontal condition and microbiology of healthy and diseased periodontal pockets in type 1 diabetes mellitus patients. J Clin Periodontol 1989;16(5):316-22. https://doi.org/10.1111/j.1600-051x.1989.tb01662.x   DOI
12 Becker W, Becker BE, Newman MG, Nyman S. Clinical and microbiologic findings that may contribute to dental implant failure. Int J Oral Maxillofac Implants 1990;5(1):31-8.
13 Botero JE, Gonzalez, AM, Mercado RA, Olave G, Contreras A. Subgingival microbiota in peri-implant mucosa lesions and adjacent teeth in partially edentulous patients. J Periodontol 2005;76(9):1490-5. https://doi.org/10.1902/jop.2005.76.9.1490   DOI
14 Yun JH, Park, JE, Kim DI, Lee SI, Choi SH, Cho KS et al. Identification of putative periodontal pathogens in Korean chronic periodontitis patients. J Korean Acad Periodontol 2008;38(2):143-52. https://doi.org/10.5051/jkape.2008.38.2.143   DOI
15 Hong JY. Periodontitis and systemic disease. J Kyung Hee Univ Med Cent 2017;32(1):1-8.
16 Al-Emadi A, Bissada N, Farah C, Siegel B, Al-Zaharani M. Systemic diseases among patients with and without alveolar bone loss. Quintessence Int 2006;37(10):761-5.
17 Sousa V, Mardas N, Farias B, Petrie A, Needleman I, Spratt D et al. A systematic review of implant outcomes in treated periodontitis patients. Clin Oral Implants Res 2016;27(7):787-844. https://doi.org/10.1111/clr.12684   DOI
18 Kigure T, Saito A, Seida K, Yamada S, Ishihara K, Okuda K. Distribution of Porphyromonas gingivalis and Treponema denticola in human subgingival plaque at different periodontal pocket depths examined by immunohistochemical methods. J Periodontal Res 1995;30(5):332-41. https://doi.org/10.1111/j.1600-0765.1995.tb01284.x.   DOI
19 Moon KH. The influence on the recognition for periodontal care to oral micro-organism changes in dental implant patients[doctoral thesis]. Cheonan: Univ. of Dankook, 2015.
20 Kim JH, Lee DI, Im SU, Jeon ES, Song KB, Choi YH. Oral health condition according to distribution of periodontopathic bacterial complex. J Korean Acad Oral Health 2020;44(1):41-7. https://doi.org/10.11149/jkaoh.2020.44.1.41   DOI
21 Lee SM, Lee HW, Lee JY. Porphyromonas gingivalis invasion of human aortic smooth muscle cells. Int J Oral Biol 2008;33(4):163-77.
22 Heitz-Mayfield LJ, Lang NP. Comparative biology of chronic and aggressive periodontitis vs. peri-implantitis. Periodontol 2000 2010;53(1):167-81. https://doi.org/10.1111/j.1600-0757.2010.00348.x   DOI
23 Gersdorf H, Meissner A, Pelz K, Krekeler G, Gobel UB. Identification of Bacteroides forsythus in subgingival plaque from patients with advanced periodontitis. J Clin Microbiol 1993;31(4):941-6. https://doi.org/10.1128/jcm.31.4.941-946.1993   DOI
24 Jang GW, Kang JB. Oral health survey method 2nd ed. Seoul: Gomoonsa; 2008: 45-8, 73.
25 Cortelli JR, Cortelli SC, Jordan S, Haraszthy VI, Zambon JJ. Prevalence of periodontal pathogens in Brazilians with aggressive or chronic periodontitis. J Clin Periodontol 2005;32(8):860-6. https://doi.org/10.1111/j.1600-051X.2005.00777.x   DOI
26 Sumida S, Ishihara K, Kishi M, Okuda K. Transmission of periodontal disease-associated bacteria from teeth to osseointegrated implant regions. Int J Oral Maxillofac Implants 2002;17(5):696-702.
27 Lee DY, Lee MS, Kwon YH, Park JB, Herr Y, Chung JH. The influence of adjacent tooth to the microbiology of implant sulcus. J Korean Acad Periodontol 2005;35(3):563-75. https://doi.org/10.5051/jkape.2005.35.3.563   DOI
28 Nishihara T, Koseki T. Microbial etiology of periodontitis. Periodontol 2000 2004;36(1):14-26. https://doi.org/10.1111/j.1600-0757.2004.03671.x   DOI
29 Park KH, Han GS. The effects of professional tooth cleaning and plaque control instruction on reduction of peri-implantitis. J Den Hyg Sci 2012;12(2):163-70.
30 Haffajee AD, Bogren A, Hasturk H, Feres M, Lopez NJ, Socransky SS. Subgingival microbiota of chronic periodontitis subjects from different geographic locations. J Clin Periodontol 2004;31(11):996-1002. https://doi.org/10.1111/j.1600-051X.2004.00597.x   DOI
31 Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent Jr RL. Microbial complexes in subgingival plaque. J Clin Periodontol 1998;25(2):134-44. https://doi.org/10.1111/j.1600-051x.1998.tb02419.x   DOI
32 Gmur R, Strub JR, Guggenheim B. Prevalence of Bacteroides forsythus and Bacteroides gingivalis in subgingival plaque of prosthodontically treated patients on short recall. J Periodontal Res 1989;24(2):113-20. https://doi.org/10.1111/j.1600-0765.1989.tb00865.x   DOI
33 Salvi GE, Aglietta M, Eick S, Sculean A, Lang NP, Ramseier CA. Reversibility of experimental peri-implant mucositis compared with experimental gingivitis in humans. Clin Oral Implants Res 2012;23(2):182-90. https://doi.org/10.1111/j.1600-0501.2011.02220.x   DOI
34 Stanley CH, Jeffrey LE. Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia: the 'red complex', a prototype polybacterial pathogenic consortium in periodontitis. Periodontol 2000 2005;38(1):72-122. https://doi.org/10.1111/j.1600-0757.2005.00113.x   DOI
35 Yakob M, Soder B, Meurman JH, Jogestrand T, Nowak J, Soder PO. Prevotella nigrescens and Porphyromonas gingivalis are associated with signs of carotid atherosclerosis in subjects with and without periodontitis. J Periodontal Res 2011;46(6):749-55. https://doi.org/10.1111/j.1600-0765.2011.01398.x   DOI
36 Flores-Guillen J, Alvarez-Novoa C, Barbieri G, Martin C, Sanz M. Five-year outcomes of a randomized clinical trial comparing bone-level implants with either submerged or transmucosal healing. J Clin Periodontol 2018;45(1):125-35. https://doi.org/10.1111/jcpe.12832   DOI
37 Zitzmann NU, Berglundh T. Definition and prevalence of peri-implant diseases. J Clin Periodontol 2008;35(s8):286-91. https://doi.org/10.1111/j.1600-051X.2008.01274.x   DOI
38 Heitz Mayfield LJ. Diagnosis and management of peri-implant diseases. Aust Dent J 2008;53(51):43-8. https://doi.org/10.1111/j.1834-7819.2008.00041.x   DOI