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http://dx.doi.org/10.13065/jksdh.20180040

Quantitative and qualitative analysis of microorganisms in dental unit water  

Lee, Seung-Hee (Department of Public Health, Graduated School of Yeungnam University)
Park, Ji-Hye (Department of Preventive Medicine and Public Health, College of Medicine, Yeungnam University)
Sakong, Joon (Department of Preventive Medicine and Public Health, College of Medicine, Yeungnam University)
Publication Information
Journal of Korean society of Dental Hygiene / v.18, no.4, 2018 , pp. 433-440 More about this Journal
Abstract
Objectives: The purpose of this study was to investigate the pathogenicity of microorganisms by quantitative and qualitative analysis of microorganisms before and after flushing of dental unit water. Methods: This study was conducted on the supply of high-speed handpieces, ultrasonic scalers, and air-water syringes, which sterilized from 10 dental unit chairs at a dental clinic in South Gyeongsang Province. The number of bacterial communities was calculated by collection before and after flushing (2, 4, and 6 minutes). Results: The mean number of bacteria in the handpiece water before flushing was $27,208CFU/m{\ell}$; 2 minutes after flushing, $2,180CFU/m{\ell}$; 4 minutes after flushing, $900CFU/m{\ell}$; and 6 minutes after flushing, $412CFU/m{\ell}$. Conclusions: To minimize the risk of cross-infection and intra-clinic infection in dental clinics, education and water quality monitoring may be needed.
Keywords
Bacteria; Cross infection; Dental clinics; Dental unit water;
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1 Ryan MP, Pembroke JT, Adley CC. Ralstonia pickettii: a persistent gram-negative nosocomial infectious organism. J Hosp Infect 2006;62(3):278-84. https://doi.org/10.1016/j.jhin.2005.08.015   DOI
2 Szymanska J, Sitkowska J, Dutkiewicz J. Microbial contamination of dental unit waterlines. Ann Agric Environ Med 2008;15(2):173-9.
3 O'Donnell MJ, Boyle MA, Russell RJ, Coleman DC. Management of dental unit waterline biofilms in the 21st century. Future Microbiol 2011;6(10):1209-26. https://doi.org/10.2217/fmb.11.104   DOI
4 Shearer BG. Biofilm and the dental office. J Am Dent Assoc 1996;127(2):181-9.   DOI
5 Centers for Disease Control and Prevention. Guidelines for infection control in dental health-care settings - 2003. Atlanta: Centers for Disease Control and Prevention; 2003: 1-76.
6 Korea Dental Association. Infection control program in the dental health care settings [Internet]. Korea Dental Association; 2007.[cited 2018 June 25]. Available from: http://www.kda.or.kr/kda/index.kda.
7 Korea Institute for Healthcare Accreditation. Dental hospital certification criteria. Korea Institute for Healthcare Accreditation; 2018[cited 2018 June 25]. Available from: https://www.koiha.or.kr/member/kr/board/establish/establish_BoardView.do.
8 Jang BS, Lee JY, Han SB. Bacterial contamination of high-speed handpiece, ultrasonic scaler and air-water syringe. J Periodontal Implant Sci 1997;27(4):941-6. https://doi.org/10.5051/jkape.1997.27.4.941   DOI
9 Yoon HY, Lee SY. The microbial contamination and effective control method of dental unit water system. J Dent Hyg Sci 2015;15(4):383-92. https://doi.org/10.17135/jdhs.2015.15.4.383   DOI
10 Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982;16(3):584-6.
11 Porteous NB, Cooley RL. Reduction of bacterial levels in dental unit waterlines. Quintessence Int 2004;35(8):630-4.
12 Szymańska J. Bacterial contamination of water in dental unit reservoirs. Ann Agric Environ Med 2007;14(1):137-40.
13 Ghosh S, Mallick SK. Microbial biofilm: contamination in dental chair unit. Indian Medical Gazette 2012;2(10):383-7.
14 Cobb CM, Martel CR, McKnight SA 3rd, Pasley-Mowry C, Ferguson BL, Williams K. How does time-dependent dental unit waterline flushing affect planktonic bacteria levels? J Dent Educ 2002;66(4):549-55.
15 Joen JS. Status of infection control behaviors of dental hygienists [Master's thesis]. Daegu: Univ. of Kyungbook National, 2012.
16 Choi SJ. A study on the status of performance of infection control standards in dental staff [Master's thesis]. Iksan: Univ. of Wonkwang, 2010.
17 Dutil S, Veillette M, Meriaux A, Lazure L, Barbeau J, Duchaine C. Aerosolization of mycobacteria and legionellae during dental treatment: low exposure despite dental unit contamination. Environ Microbiol 2007;9(11):2836-43.   DOI
18 Kelley ST, Theisen U, Angenent LT, St Amand A, Pace NR. Molecular analysis of shower curtain biofilm microbes. Appl Environ Microbiol 2004;70(7):4187-92. https://doi.org/10.1128/AEM.70.7.4187-4192.2004   DOI
19 Kim YH, Koh EM, Lee YW, Lee YK, Ghin HB, Lee KW. Pseudo-outbreak of Brevundimonas diminuta. Korean J Clin Microbiol 2011;14(3):115-7.   DOI
20 Gini GA. Ocular infection caused by Psychrobacter immobilis acquired in the hospital. J Clin Microbiol 1990;28(2):400-1.
21 Chmel H. Serratia odorifera biogroup 1 causing an invasive human infection. J Clin Microbiol 1988;26(6):1244-5.
22 Lee BM, Kim CW, Kim YS. A study on the microbial contamination of dental unit and ultrasonic scaler. J Korean Acad Prosthodont 1998;36(1):64-80.
23 Kang EJ, Kang HS, Kwak JS, Kim SM, Kim SH, Moon SE, et al. Infection control in the dental office. 2nd ed. Seoul: Daehannarae publishing inc.; 2009:22-3.
24 Blake GC. The incidence and control of bacterial infection of dental unit and ultrasonic scales. Br Dent J 1963;15(1):413-6.
25 Tyagi S, Kulkarni P, Prasad KP. Science regarding dental unit waterlines (DUWL): A review. Annals and Essences of Dentistry 2010;2(3):117-22.   DOI
26 PD Note. dangerous secrets of dentistry [Internet]. MBC; 2006.[cited 2018 June 25]. Available from: http://www.imbc.com/broad/tv/culture/pd/vod/.
27 20/20. Dirty Dental Water [Internet]. ABC News; 2000.[cited 2000 February 18]. Available from: http://qater.co.kr/index.php/810.
28 Mills SE. The dental unit waterline controversy: defusing the myths, defining the solutions. J Am Dent Assoc 2000;131(10):1427-41.   DOI
29 Han JS. A study about the contamination of microoranisms in private dental clinic [Doctoral dissertation]. Cheonan: Univ. of Dankook, 2002.
30 Karlsson MD, Jacobsson B. Intrauterine fetal death associated with Rothia dentocariosa: a case report. Am J Obstet Gynecol 2007;197(5):e6-7. https://doi.org/10.1016/j.ajog.2007.08.024   DOI
31 Ricaurte JC, Klein O, LaBombardi V, Martinez V, Serpe A, Joy M. Rothia dentocariosa endocarditis complicated by multiple intracranial hemorrhages. South Med J 2001;94(4):438-40.   DOI