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http://dx.doi.org/10.13065/jksdh.2017.17.03.441

Effect of Cimicifuga rhizoma extract on the odontoblastic differentiation of MDPC-23 cells  

Choi, Byul-Bora (Department of Oral Anatomy, School of Dentistry, Pusan National University)
Kim, Ji-Young (Department of Dental Hygiene, Kyungnam College of Information & Technology)
Park, Sang-Rye (Department of Dental Hygiene, Kyungnam College of Information & Technology)
Publication Information
Journal of Korean society of Dental Hygiene / v.17, no.3, 2017 , pp. 441-448 More about this Journal
Abstract
Objectives: The purpose of this study was to examine the cell proliferation and expression of alkaline phosphatase (ALP) during the differentiation of murine odontoblast-like cells (MDPC-23) by Cimicifuga rhizoma extract. Cimicifuga rhizoma extract was prepared using 70% ethanol. Then, the cells were treated with 25, 50, 100, 150, and $200{\mu}g$ of Cimicifuga rhizoma extract. Methods: We determined the Cimicifuga rhizoma effects of MDPC-23 using WST-1 (water soluble tetrazolium salt-1) assay, ALP activity assay and histochemical staining. Results: $25-200{\mu}g$ of Cimicifuga rhizoma extract did not inhibit the growth of MDPC-23 cells; $100{\pm}0$, $100{\pm}3.29$, $99{\pm}4.86$, $98{\pm}3.80$, $98{\pm}1.73$, $99{\pm}5.05%$ (p<0.794). $50{\mu}g$ of Cimicifuga rhizoma extract stimulated ALP activity on MDPC-23; $5.1{\pm}0.20units/{\mu}{\ell}$ (p<0.001). Conclusions: It was proven that Cimicifuga rhizoma promoted differentiation of MDPC- 23 cells.
Keywords
Alkaline phosphatase; Cimicifuga rhizoma; Differentiation; Odontoblast;
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