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http://dx.doi.org/10.13065/jksdh.2017.17.01.13

Analysis of total oral microorganisms in saliva using real-time PCR and colony forming unit  

Yoo, Su-Min (Departments of Dental Hygiene, Kyungdong University)
Jeong, Seong-Kug (Departments of Preventive Dentistry, School of Dentistry, Chosun University)
Yoo, Hyun-Jun (Departments of Preventive Dentistry, School of Dentistry, Dankuk University)
Jang, Jong-Hwa (Departments of Dental Hygiene, Hanseo University)
Publication Information
Journal of Korean society of Dental Hygiene / v.17, no.1, 2017 , pp. 13-25 More about this Journal
Abstract
Objectives: The purpose of this study was to compare colony forming unit (CFU) method and multiplex real-time polymerase chain reaction (MRT-PCR) method for accurate quantitative analysis of bacteria. Methods: We compared the CFU method and the MRT-PCR method, which are still used in Korea, for Prevotella intermedius (P. intermedius), a periodontal disease pathogen selected by MRT-PCR, and Streptococcus mutans (S. mutans), a dental caries causative organism. The subjects of this study were 30 patients who visited the C dental hospital. Results: Total microorganisms in MRT-PCR method were significantly higher in both types of bacteria (p<0.05), since DNA of dead bacteria was also analyzed. This was because the periodontal dise(-) anaerobes, and even dead bacteria contain large amounts of toxic substances called LPS in the extracellular membrane, and fimbriae and pili, which are motility structures, still remain as a strong toxic substance in periodontal tissue. Conclusions: Therefore, in terms of the total amount of bacteria found, the MRT-PCR method will be a useful technique for searching all the bacteria in the oral cavity including live bacteria, as well as sterilization.
Keywords
Colony forming unit; Multiplex real-time polymerase chain reaction (MRT-PCR); Oral microorganisms; Saliva;
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