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http://dx.doi.org/10.13065/jksdh.2015.15.06.1063

Comparison of quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction  

Kim, Young-Sun (Department of Dental Hygiene, Daegu Health College)
Lee, Jung-Hwa (Department of Dental Hygiene, Daegu Health College)
Lee, Young-Eun (Department of Dental Hygiene, Daegu Health College)
Publication Information
Journal of Korean society of Dental Hygiene / v.15, no.6, 2015 , pp. 1063-1071 More about this Journal
Abstract
Objectives: The purpose of the study is to investigate the quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction. Methods: Participants were voluntarily recruited at D university, and saliva samples were extracted before and after scaling. Multiple real-time polymerase chain reactions were used to analyze characteristics and the amount of nine kinds of periodontal pathogens; Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia, Fusobacterium nucleatum, Parvimonas micra, Campylobacter rectus, and Eikenella corrodens. Results: After scaling, most periodontal pathogens except Eikenella corrodens were significantly decreased in all subjects(p<0.05). In addition, the percentage of microorganisms associated with disease, the microorganism risk index of periodontitis and the prevalence of red complex, orange complex, and Aggregatibacter actinomycetemcomitans was also significantly reduced after scaling(p<0.05). Conclusions: Scaling decreased in the amount of major periodontal pathogens and periodontitis prevalence rate.
Keywords
periodontal pathogens; real-time polymerase chain reaction; scaling;
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