Browse > Article
http://dx.doi.org/10.5395/rde.2020.45.e20

Influence of pain-relieving therapies on inflammation and the expression of proinflammatory neuropeptides after dental bleaching treatment  

da Silva, Livia Maria Alves Valentim (Department of Restorative Dentistry, Sao Paulo State University (UNESP), School of Dentistry)
Cintra, Luciano Tavares Angelo (Department of Restorative Dentistry, Sao Paulo State University (UNESP), School of Dentistry)
de Oliveira Gallinari, Marjorie (Department of Restorative Dentistry, Sao Paulo State University (UNESP), School of Dentistry)
Benetti, Francine (Department of Restorative Dentistry, Universidade Federal de Minas Gerais (UFMG))
Rahal, Vanessa (Department of Restorative Dentistry, Sao Paulo State University (UNESP), School of Dentistry)
Ervolino, Edilson (Department of Basic Sciences, Sao Paulo State University (UNESP), School of Dentistry)
de Alcantara, Sibele (Department of Restorative Dentistry, Sao Paulo State University (UNESP), School of Dentistry)
Briso, Andre Luiz Fraga (Department of Restorative Dentistry, Sao Paulo State University (UNESP), School of Dentistry)
Publication Information
Restorative Dentistry and Endodontics / v.45, no.2, 2020 , pp. 20.1-20.14 More about this Journal
Abstract
Objectives: To minimize the tooth sensitivity caused by in-office bleaching, many dentists use non-steroidal anti-inflammatory drugs and topical desensitizing gels containing potassium nitrate and sodium fluoride. This study aimed to evaluate the influence of these substances on inflammation and the expression of substance P and calcitonin gene-related peptide in pulp nerve fibers. Materials and Methods: Seventy-two rats were divided into 6 groups as follows: GI, control; GII, only dental bleaching; GIII, only ibuprofen; GIV, ibuprofen administered 30 minutes before and after the bleaching treatment and every 12 hours until the analysis; GV, only topical application of a desensitizing agent; and GVI, topical application of a desensitizing agent before dental bleaching. Placebo gel was applied to the upper left jaw and the bleaching agent was applied to the upper right jaw in all groups. Subsequently, the groups were divided into 3 subgroups based on the time of analysis: 0, 24, and 48 hours after bleaching (n = 8). The rats were euthanized and the maxillae were processed and evaluated by histopathological and immunohistochemical analyses. The data were analyzed using the Kruskal-Wallis test, followed by the Dunn test (p < 0.05). Results: In the bleaching groups, the inflammatory process and expression of neuropeptides decreased over time. The animals in which a desensitizing agent was applied showed better results within 24 hours. Conclusions: The use of a desensitizing agent had positive effects on inflammation and pain-related neuropeptide expression, minimizing the painful effects of dental bleaching treatment.
Keywords
Tooth bleaching; Hydrogen peroxide; Ibuprofen; Laboratory research; Pain; Reactive oxygen species;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Schafers M, Marziniak M, Sorkin LS, Yaksh TL, Sommer C. Cyclooxygenase inhibition in nerve-injury- and TNF-induced hyperalgesia in the rat. Exp Neurol 2004;185:160-168.   DOI
2 Benetti F, Briso ALF, Carminatti M, de Araujo Lopes JM, Barbosa JG, Ervolino E, Gomes-Filho JE, Cintra LTA. The presence of osteocalcin, osteopontin and reactive oxygen species-positive cells in pulp tissue after dental bleaching. Int Endod J 2019;52:665-675.   DOI
3 Lillie RD. Histopathologic technic and practical histochemistry. 2nd ed. New York: Blakinston; 1954. p501.
4 Gallinari MO, Cintra LT, Benetti F, Rahal V, Ervolino E, Briso AL. Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs. PLoS One 2019;14:e0210338.   DOI
5 Ferreira VG, Nabeshima CK, Marques MM, Paris AF, Gioso MA, dos Reis RS, Machado ME. Tooth bleaching induces changes in the vascular permeability of rat incisor pulps. Am J Dent 2013;26:298-300.
6 de Almeida LC, Soares DG, Gallinari MO, de Souza Costa CA, Dos Santos PH, Briso AL. Color alteration, hydrogen peroxide diffusion, and cytotoxicity caused by in-office bleaching protocols. Clin Oral Investig 2015;19:673-680.   DOI
7 Cintra LT, Benetti F, Ferreira LL, Rahal V, Ervolino E, Jacinto RC, Gomes Filho JE, Briso AL. Evaluation of an experimental rat model for comparative studies of bleaching agents. J Appl Oral Sci 2016;24:95-104.
8 Nelson-Filho P, Lucisano MP, Da Silva RA, Da Silva RS, Serra MC, Gerlach RF, Neto FC, Carneiro ZA, Zamarioli A, Morse L, Battaglino R. Systemically alendronate was incorporated into dental tissues but did not cause morphological or mechanical changes in rats teeth. Microsc Res Tech 2012;75:1265-1271.   DOI
9 Cintra LTA, Benetti F, Rahal V, Facundo ACS, Ferreira LL, Gomes-Filho JE, Ervolino E, Briso ALF, editors. Influence of dental bleaching sessions on the pulp of rats. IADR/AADR/CADR 91st General Session and Exhibition; 2013 Mar 20-23; Seattle, WA. Alexandria: International Association for Dental Research (IADR); 2013.
10 Tay LY, Kose C, Loguercio AD, Reis A. Assessing the effect of a desensitizing agent used before in-office tooth bleaching. J Am Dent Assoc 2009;140:1245-1251.   DOI
11 Caviedes-Bucheli J, Lombana N, Azuero-Holguin MM, Munoz HR. Quantification of neuropeptides (calcitonin gene-related peptide, substance P, neurokinin A, neuropeptide Y and vasoactive intestinal polypeptide) expressed in healthy and inflamed human dental pulp. Int Endod J 2006;39:394-400.   DOI
12 Awawdeh LA, Lundy FT, Linden GJ, Shaw C, Kennedy JG, Lamey PJ. Quantitative analysis of substance P, neurokinin A and calcitonin gene-related peptide in gingival crevicular fluid associated with painful human teeth. Eur J Oral Sci 2002;110:185-191.   DOI
13 Caviedes-Bucheli J, Munoz HR, Azuero-Holguin MM, Ulate E. Neuropeptides in dental pulp: the silent protagonists. J Endod 2008;34:773-788.   DOI
14 Rahal V, Gallinari MO, Perdigao J, Cintra LT, dos Santos PH, Briso AL. Quantitative sensory testing of the effect of desensitizing treatment after dental bleaching. Acta Odontol Latinoam 2015;28:263-270.
15 Cintra LT, Benetti F, Ferreira LL, Gomes-Filho JE, Ervolino E, Gallinari MO, Rahal V, Briso AL. Penetration capacity, color alteration and biological response of two in-office bleaching protocols. Braz Dent J 2016;27:169-175.   DOI
16 de Almeida LC, Costa CA, Riehl H, dos Santos PH, Sundfeld RH, Briso AL. Occurrence of sensitivity during at-home and in-office tooth bleaching therapies with or without use of light sources. Acta Odontol Latinoam 2012;25:3-8.
17 Soares DG, Basso FG, Hebling J, de Souza Costa CA. Concentrations of and application protocols for hydrogen peroxide bleaching gels: effects on pulp cell viability and whitening efficacy. J Dent 2014;42:185-198.   DOI
18 Caviedes-Bucheli J, Ariza-Garcia G, Restrepo-Mendez S, Rios-Osorio N, Lombana N, Munoz HR. The effect of tooth bleaching on substance P expression in human dental pulp. J Endod 2008;34:1462-1465.   DOI
19 Cintra LT, Benetti F, da Silva Facundo AC, Ferreira LL, Gomes-Filho JE, Ervolino E, Rahal V, Briso AL. The number of bleaching sessions influences pulp tissue damage in rat teeth. J Endod 2013;39:1576-1580.   DOI
20 Benetti F, Gomes-Filho JE, Ferreira LL, Ervolino E, Briso AL, Sivieri-Araujo G, Dezan-Junior E, Cintra LT. Hydrogen peroxide induces cell proliferation and apoptosis in pulp of rats after dental bleaching in vivo: effects of the dental bleaching in pulp. Arch Oral Biol 2017;81:103-109.   DOI
21 Almeida LC, Riehl H, Santos PH, Sundfeld ML, Briso AL. Clinical evaluation of the effectiveness of different bleaching therapies in vital teeth. Int J Periodontics Restorative Dent 2012;32:303-309.
22 Lundberg JM, Pernow J, Tatemoto K, Dahlof C. Pre- and postjunctional effects of NPY on sympathetic control of rat femoral artery. Acta Physiol Scand 1985;123:511-513.   DOI
23 Pashley DH. How can sensitive dentine become hypersensitive and can it be reversed? J Dent 2013;41 Supplement 4:S49-S55.   DOI
24 Brain SD, Williams TJ, Tippins JR, Morris HR, MacIntyre I. Calcitonin gene-related peptide is a potent vasodilator. Nature 1985;313:54-56.   DOI
25 Casasco A, Calligaro A, Casasco M, Springall DR, Polak JM, Poggi P, Marchetti C. Peptidergic nerves in human dental pulp. An immunocytochemical study. Histochemistry 1990;95:115-121.   DOI
26 Haywood VB. Treating sensitivity during tooth whitening. Compend Contin Educ Dent 2005;26:11-20.
27 Armenio RV, Fitarelli F, Armenio MF, Demarco FF, Reis A, Loguercio AD. The effect of fluoride gel use on bleaching sensitivity: a double-blind randomized controlled clinical trial. J Am Dent Assoc 2008;139:592-597.   DOI
28 Haywood VB, Heymann HO. Nightguard vital bleaching: how safe is it? Quintessence Int 1991;22:515-523.
29 Perdigao J. Dental whitening--revisiting the myths. Northwest Dent 2010;89:19-21.
30 Williams HA, Rueggeberg FA, Meister LW. Bleaching the natural dentition to match the color of existing restorations: case reports. Quintessence Int 1992;23:673-677.
31 Anderson DG, Chiego DJ Jr, Glickman GN, McCauley LK. A clinical assessment of the effects of 10% carbamide peroxide gel on human pulp tissue. J Endod 1999;25:247-250.   DOI
32 Pale M, Mayoral JR, Llopis J, Valles M, Basilio J, Roig M. Evaluation of the effectiveness of an in-office bleaching system and the effect of potassium nitrate as a desensitizing agent. Odontology 2014;102:203-210.   DOI
33 Kina JF, Huck C, Riehl H, Martinez TC, Sacono NT, Ribeiro AP, Costa CA. Response of human pulps after professionally applied vital tooth bleaching. Int Endod J 2010;43:572-580.   DOI
34 Costa CA, Riehl H, Kina JF, Sacono NT, Hebling J. Human pulp responses to in-office tooth bleaching. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:e59-e64.
35 Basting RT, Amaral FL, Franca FM, Florio FM. Clinical comparative study of the effectiveness of and tooth sensitivity to 10% and 20% carbamide peroxide home-use and 35% and 38% hydrogen peroxide in-office bleaching materials containing desensitizing agents. Oper Dent 2012;37:464-473.   DOI
36 Paula E, Kossatz S, Fernandes D, Loguercio A, Reis A. The effect of perioperative ibuprofen use on tooth sensitivity caused by in-office bleaching. Oper Dent 2013;38:601-608.   DOI
37 Charakorn P, Cabanilla LL, Wagner WC, Foong WC, Shaheen J, Pregitzer R, Schneider D. The effect of preoperative ibuprofen on tooth sensitivity caused by in-office bleaching. Oper Dent 2009;34:131-135.   DOI