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http://dx.doi.org/10.5395/JKACD.2011.36.3.196

Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth  

Shin, Su-Jung (Department of Conservative Dentistry, College of Dentistry, Yonsei University)
Lee, Woo-Cheol (Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University)
Lee, Jae-Il (Department of Oral Pathology, School of Dentistry, Seoul National University)
Baek, Seung-Ho (Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University)
Kum, Kee-Yeon (Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University)
Shon, Won-Jun (Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University)
Bae, Kwang-Shik (Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University)
Publication Information
Restorative Dentistry and Endodontics / v.36, no.3, 2011 , pp. 196-202 More about this Journal
Abstract
Objectives: The aim of this study was to investigate levels of matrix metalloproteinase-8 (MMP-8) and substance P (SP) in root canal exudates during root canal treatment (RCT) of nonvital, painful teeth. Materials and Methods: Patients scheduled for nonsurgical RCT were prospectively selected; the study was performed after obtaining informed consent from the patients and was approved by the Institutional Review Board for Clinical Research of Gangnam Severance Hospital, Yonsei University (3-2008-0118). Canal exudates samples were collected using sterilized paper points from teeth scheduled for RCT across three different time periods. MMP-8 and SP levels were measured using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a mixed model analysis and the Pearson correlation analysis (p < 0.05). Results: MMP-8 and SP levels in GCF were decreased during RCT (p < 0.0001), and they showed a weak positive correlation to each other (p < 0.05). Patients' subjective pain levels and the response from percussion test were significantly related to SP level. Conclusions: This study demonstrated that periradicular inflammation endodontic origin can elevate SP and MMP-8 levels in root canal exudates. Interestingly, SP level of canal exudates showed a possibility of being used as an indicator of pain due to periapical pathosis.
Keywords
Canal exudate; MMP-8; SP; Pain;
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