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http://dx.doi.org/10.5395/JKACD.2009.34.2.120

Histological evaluation of direct pulp capping with DSP-derived synthetic peptide in beagle dog  

Kim, Jae-Hoon (Department of Conservative Dentistry, School of Dentistry, Seoul National University)
Hong, Jun-Bae (Department of Conservative Dentistry, School of Dentistry, Seoul National University)
Lim, Bum-Soon (Department of Dental Biomaterials Science, School of Dentistry, Seoul National University)
Cho, Byeong-Hoon (Department of Conservative Dentistry, School of Dentistry, Seoul National University)
Publication Information
Restorative Dentistry and Endodontics / v.34, no.2, 2009 , pp. 120-129 More about this Journal
Abstract
The purpose of this study was to investigate the pulpal response to direct pulp capping with dentin sialoprotein (DSP) -derived synthetic peptide in teeth of dogs, and to compare its efficacy to capping substances $Ca(OH)_2$ and white mineral trioxide aggregate (WMTA). A total of 72 teeth of 6 healthy male beagle dogs were used. The mechanically exposed pulps were capped with one of the following: (1) DSP-derived synthetic peptide (PEP group): (2) $Ca(OH)_2$ (CH group): (3) a mixture paste of peptide and $Ca(OH)_2$ (PEP+CH group): or (4) white MTA (WMTA group). The access cavity was restored with a reinforced glass ionomer cement. Two dogs were sacrificed at each pre-determined intervals (2 weeks, 1 month, and 3 months). After the specimens were prepared for standard histological processing, sections were stained with hematoxylin and eosin. Under a light microscope, inflammatory response and hard tissue formation were evaluated in a blind manner by 2 observers. In the PEP group, only 3 of 17 specimens showed hard tissue formation, indication that the DSP-derived synthetic peptide did not induce proper healing of the pulp. Compared with the CH group, the PEP group demonstrated an increased inflammatory response and poor hard tissue formation. The CH and WMTA groups showed similar results for direct pulp capping in mechanically exposed teeth of dogs.
Keywords
Pulp capping; Dentin sialoprotein; Reparative dentin; Histological evaluation; Calcium hydroxide; Beagle dog;
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  • Reference
1 Goldberg M, Six N, Decup F, Lasfargues JJ , Salih E,Tompkins K. Veis A. Bioactive molecules and the future of pulp therapy. Am J Dent 16:66-76, 2003   PUBMED
2 Schuurs AHB, Gruythuysen RJM, Wesselink PR, Pulp capping with adhesive resin based composite versus calcium hydroxide: a review. Endod Dent Traumatol 16:240-250, 2000   DOI
3 Dominguez MS' Witherspoon DE. Gutmann JL, Opperman LA' Histological and scanning electron microscopy assessment of various vital pulp-therapy materials. J Endod 29:324-333. 2003   DOI   ScienceOn
4 Iwamoto CE. Adachi E. Pameijer CH, Barnes D, Romberg EE, Jefferies S. Clinical and histological eval uation of white ProRoot MTA in direct pulp capping. Am J Dent 19:85-90, 2006
5 Goldberg M, Six N, Decup F, et al. Application of bioactive molecules in pulp-capping situations. Adv Dent Res 15:91-95, 2001   DOI   ScienceOn
6 Six N, Decup F, Lasfargues JJ , Salih E, Goldberg M, Osteogenic proteins (bone sialoprotein and bone morphogenetic protein-7) and dental pulp mineralization. J Mater Sci Mater Med 13:225-232. 2002   DOI   ScienceOn
7 Tziafas D, Kolokuris I, Alvanou A, Kaidoglou K. Shortterm dantinogenic response of dog pulp tissue after its induction by demineralized or native dentine or by predentine. Arch Oral Biol 37:119-128. 1992   DOI   ScienceOn
8 Stanley HR, Pameijer CR Dentistry's friend, calcium hydroxide. Oper Dent 22:1-3, 1997   DOI   PUBMED
9 Cox CF, Bergenholtz G, Fitzgerald M, Heys DR. Heys RJ‘ Avery JK, Baker JA, Capping of the dental pulp mechanically exposed to the oral microf1ora: 5-week observation of wound healing in the monkey, J Oral Pathol 11:327-339. 1982   DOI   PUBMED
10 Torabinejad M, Pitt Ford Tf( McKendry DJ, Abodi HR, Miller DA, Kariyawasam SP. Histological assoss ment of mineral trioxide aggregate as a root-end filling in monkeys. J Endod 23:225-228, 1997   DOI   ScienceOn
11 Myers K, Kaminski E, Lautenschalger E, Miller D, The effects of mineral trioxide aggregate on the dog pulp (Abstract), J Endod 22:198, 1996
12 Herman BW. Dentin obliteration after treatment with calcium. Zahnarztl Rundschau 39:888-899. 1930. (In German)
13 Butler WT. Sialoprotein of bone and dentin. J Bio Buccale 19:83-89, 1991   PUBMED
14 Cox CF, Bergenholtz G, Heys DR, Syed SA, Fitzgerald M, Heys RJ. Pulp-capping of dental pulp mechanically exposed to oral microf1ora: a 1-to-2-year observation of wound healing in the monkey. J Oral Pathol 14:156-168, 1985   DOI   PUBMED
15 Song JS , Mante FI\, Romanow WJ, Kim S, Chemical analysis of powder and set forms of Port.1and cement, gray ProRoot MTA, white ProRoot MTA, and gray MTA-Angelus. Oral Surg Oral Med Oral Pathol Oral Radial Endod 102:809-815, 2006   DOI   ScienceOn
16 Nair PNR. Duncan HF. Pitt Ford TR. Luder HU. Histological. ultrastructural and quantitative investigations on the response of health human pulps to experimental capping with mineral trioxide aggregate: a randomized controlled trial. Int Endod J 41:128-150. 2008   DOI   PUBMED   ScienceOn
17 Heithersay GS. Calcium hydroxide in the treatment of pulpless teeth with associated pathology. J Br Endod Soc 8:74-93, 1975   DOI   PUBMED
18 Lee SJ , MonsefM, Torabinejad M. Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod 19:541-544, 1993   DOI   ScienceOn
19 Siqueil’a JR. Lopez HP. Mechanism of antimicrobial activity of calcium hydroxide: a critical review‘ Int Endod 32:361-369, 1999   DOI
20 Wang J , Kennedy JG. Glimcher M,J, Salih E. Novel bioactive property of purified native bone sialoprotein in bone repair of calvarial defect. Trans Orthop Res Soc 23:1007, 1998
21 Decup F. Six N, Palmier B, Buch D. Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat's molar. Clin Oral Invest 4: 110-119. 2000   DOI   ScienceOn
22 Holland R, De souza V, Nevy NJ, Otobone Filho JA, Bernabo DF, Dozan Jr E. Hoaction of rat connective tissue to implanted dentin tubes filled with mineral trioxide aggrogate or calcium hydoxide , J Endod 25:161-166. 1999   DOI   ScienceOn
23 Wu MK. Kontakiotis EG, WesseIink PR. Long-term soal providod by somo root-end fillng materials. J Endod 24:557-560, 1998   DOI   ScienceOn
24 Schroder U. Evaluation of healing following experimental pulpotomy of intact human teeth and capping with calcium hydroxide. OdoIltol Revy 23:329-340, 1972   PUBMED
25 Fischer EJ , Arens DE, MillorCR Bacterial leakage of mineral trioxide aggregato as compared with zinc-froo amalgam , intermediate restorative material. and Super-EBA as a root-ond filling material. J Endod 24:176-179, 1998   DOI   ScienceOn
26 Abedi HR, Torabinejad M, Pitt Ford TR. Bakland LK. The use of mineral trioxide aggregate cement as a direct pulp capping material J Am Dent Assoc 127: 1491-1494, 1996
27 Parirokh M, Asgary S, Eghb잉 MJ, Stowe S, Eslami B. Eskandarizade A‘ Shabahang S. A comparativo study of white and grey mineral trioxide aggregate as pulp capping agents in dog's teeth. Dent Traumatol 21:150-154, 2005   DOI   ScienceOn
28 Schroder U. Effects of calcium hydroxide-containing pulp-capping agents on pulp cell migration, prolifera tion and differentiation. J Dent Res 64(suppl. 1): 541-548, 1985   DOI
29 George A. Bannon L. Sabsay B, Dillon JW, Malone J, Veis A, Jenkins NA, Gilbert OJ, Copeland NG. The carboxyHerminal domain of phosphophoryn contains unique extended triplet amino acid repeat sequences forming ordered carboxyl-phosphate interaction ridges that may be essential in the biomineralization process. J BioI Chern 271:32869-32873. 1996   DOI   ScienceOn
30 Pitt Ford TR, Torabinejad M, Abedi HR. Bakland LK. Kariyawasam SP. Using mineral trixoide aggregate as a pulp capping material. J Am Dent Assoc 127:1491-1494, 1996   DOI
31 Keiser K. Johnson CC, Tipton DA, Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fiboroblasts. J EIldod 26:288-291. 2000   DOI   ScienceOn
32 Aeinehchi M, Eslami B, Ghanbariha M, Saffar AS. Mineral trioxide aggregate (MTA) and calcium hydroxide as pulp-capping agents in human teeth: a preliminary report. Int Endod J 36:225-231. 2002   DOI   ScienceOn
33 TorabinejadM, Watson EF, Pitt Ford TR Sealing abiI. ity. of minoral trioxide aggregate when used as a root end filling material J Endod 19:591-595, 1993   DOI   ScienceOn
34 Agamy HA, Bakry NS, Mounir MF, Avery DR. Comparison of mineral trioxide aggrega t.e and formocresol as pulp-capping agents in pulpotomized primary teeth. Pediatr Dent 26:302-309, 2004   PUBMED
35 Pitt Ford TR, Torabinejad M, Albedi HR, Bakland LK. Kariyawasam SP. Using mineral trioxide aggregate as a pulp-capping material. J Am Dent Assoc 127:1491-1494. 1996   DOI
36 Holland R, de Souza V, Nory Mι Otoboni Filho JA, Bernabe PF, Dezan Jr E‘ Reaction of dog’s teeth to root canal filling with mineral trioxide aggregate or a glass ionomer sealer. J Endod 25:728-730, 1999   DOI   ScienceOn
37 Maroto M, Barberia E, Vera V, Garcia-Godoy F Dentin bridge formation after white trioxide aggregate (white MTA) pulpotomies in primary molars. Am J Dent 19:75-79, 2006   PUBMED
38 Qin C, Brunn JC , Ridall A. et aI. the expression of dentin sialophosphoprotein gene in bone. J Dent Res 81:392-394, 2002   DOI   ScienceOn