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http://dx.doi.org/10.5395/JKACD.2005.30.2.138

Effect of Sonicated Extracts of Enterococcus faecalis on the Production of Matrix Metalloproteinase-8 by Human Polymorphonuclear Neutrophils  

Park, Sung-Kyung (Department of Conservative Dentistry, College of Dentistry, Seoul National University)
Shon, Won-Jun (Department of Conservative Dentistry, College of Dentistry, Seoul National University)
Lim, Sung-Sam (Department of Conservative Dentistry, College of Dentistry, Seoul National University)
Publication Information
Restorative Dentistry and Endodontics / v.30, no.2, 2005 , pp. 138-144 More about this Journal
Abstract
This in vitro study monitored MMP-8 production on PMN by stimulated with the following three groups; Sonicated extracts of E. faecalis (SEF), SEF treated with $Ca(OH)_2$ (12.5mg/ml) for 7 days, and lipopolysac-charides(LPS) of E. coli. The level of MMP-8 in each group was immediately measured by ELISA. The data were analyzed with Kruskal-Wallis test and Mann-Whitney U test. In the SEF group, the level of production of MMP-8 was higher than the negative control group in low concentration ($0.05{\mu}g/ml$) of SEF (p < 0.05). but it decreased with an increase in the concentration of SEF (p < 0.05). In the case of SEF treated with $Ca(OH)_2$, all of the MMP levels were higher than negative control group (p < 0.05), but no statistical difference was found among the different SEF concentrations (p > 0.05). All of the levels in E. coli LPS were incresed with increasing concentrations (p < 0.05). According to this study we could summarized as follows: 1. MMP-8 was expressed at low level in untreated PMN group the levels of MMP-8 were upregulated in PMN stimulated by E. coli LPS groups. 2. In the SEF groups, the level of production of MMP-8 decreased with an increase in concentration of SEF (p < 0.05). So E. faecalis may have suppressive effect on the production of MMP-8 by PMN. 3. In the case of SEF treated with $Ca(OH)_2$, all of the MMP levels at different SEF concentrations were higher than untreated PMN group (p < 0.05), but no statistical difference was found among the different SEF concentrations (p > 0.05).
Keywords
E. faecalis; MMP-8; Calcium hydroxide; PMN leukocyte;
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1 Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rat. Oral Burg Oral Med Oral Pathol 20:340-4, 1965   DOI   ScienceOn
2 Hanemaaijer R, Sorsa T, Konttinen YT, Ding Y, Sutinen M, Visser H, van Hinsbergh VW, Helaakoski T, Kainulainen T, Ronka H, Tschesche H, Salo T. Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells. Regulation by tumor necrosis factor-alpha and doxycycline. J Biol Chem Dec 272:31504-9, 1997   DOI
3 Son HH, Lim S, Shon W, Kim HS, Lee W. Effects of sonicated Enterococcus faecalis extracts on Interleukin-2 and Interleukin-4. J Endod 30(10):701-3, 2004   DOI   ScienceOn
4 Jeansonne MJ. White RRA comparison of 2.0% chlorhexidine gluconate and 5.25% sodium hypochlorite as antimicrobial endodontic irrigants. J Endod 20:276-8, 1994   DOI   ScienceOn
5 Wang JD, Hume WR. Diffusion of hydrogen ion and hydroxyl ion from various sources through dentine. Int Endod J 21:17-26, 1988   DOI   PUBMED
6 Haapasalo HK, Siren EK, Waltimo TM, Orstavik D, Haapasalo MP. Inactivation of local root canal medicaments by dentine: an in vitro study. Int Endod J 33:126-31, 2000   DOI   ScienceOn
7 Sjogren U, Figdor D, Spangberg L, Sundqvist G. The antimicrobial effect of calcium hydroxide as a shortterm intracanal dressing. Int Endod J 24:119-25, 1991   DOI   PUBMED
8 Safavi KE, Spangberg LS, Langeland K. Root canal dentinal tubule disinfection. J Endod 16:207-10, 1990   DOI   ScienceOn
9 Wahlgren J, Salo T, Teronen O, Luoto H, Sorsa T, Tjaderhane L. Matrix metalloproteinase-8 (MMP-8) in pulpal and periapical inflammation and periapical rootcanal exudates. Int Endod J 35:897-904, 2002   DOI   ScienceOn
10 Haapasalo M, Orstavik D, In vitro infection and disinfection of dentinal tubules. J Dent Res 66:1375-9, 1987   DOI   ScienceOn
11 Evans M, Davies JK, Sundqvist G, Figdor D. Mechanisms involved in the resistance of Enterococcus faecalis to cillcium hydroxide. Int Endod J 35:221-8, 2002   DOI   ScienceOn
12 Heithersay GS. Calcium hydroxide in the treatment of pulpless teeth with associated pathology. J Br Endod Soc 8:74-93, 1975   DOI   PUBMED
13 Nerwich A, Figdor D, Messer HH. pH changes in root dentin over a 4-week period following root canal dressing with calcium hydroxide. J Endod 19:302-6, 1993   DOI   ScienceOn
14 Mainardi CL, Pourmotabbed TF, Hasty KA. Inflammatory phagocytes and connective tissue degrading metalloproteinases. Am J Med Sci 302:171-5, 1991   DOI   ScienceOn
15 Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo M. Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J 34:429-34, 2001   DOI   ScienceOn
16 Delany GM, Patterson SS, Miller CH, Newton CW. The effect of chlorhexidine gluconate irrigation on the root canal flora of freshly extracted necrotic teeth. Oral Surg Oral Med Oral Pathol 53:518-23, 1982   DOI   ScienceOn
17 Shin SJ, Lee JI, Baek SH, Lim SS. Tissue levels of matrix metalloproteinases in pulps and periapical lesions. J Endod 28:313-5, 2002   DOI   ScienceOn
18 Hartke A, Giard JC, Laplace JM, Auffray Y. Survival of Enterococcus faecalis in an oligotrophic microcosm: changes in morphology, development of general stress resistance, and analysis of protein synthesis. Appl Environ Microbiol 64:4238-45, 1998
19 Orstavik D, Haapasalo M. Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol 6:142-9, 1990   DOI
20 Fabricius L, Dahlen G, Holm SE, Moller AJ. Influence of combinations of oral bacteria on periapical tissues of monkeys. Scand J Dent Res 90:200-6, 1982
21 Yoshida H, Jontell M, Sundqvist G, Bergenholtz G. Effect of sonicated material from Fusobacterium nucleatum on the functional capacity of accessory cells derived from dental pulp. Oral Microbiol Immunol 10:208-12, 1995   DOI   ScienceOn
22 Siqueira Junior JF, Batista MM, Fraga RC, de Uzeda M. Antibacterial effects of endodontic irrigants on black-pigmented gram-negative anaerobes and facultative bacteria. J Endod 24:414-6, 1998   DOI   ScienceOn
23 Molander A, Reit C, Dahlen G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J 31:1-7, 1998   DOI   ScienceOn
24 Vahdaty A, Pitt Ford TR, Wilson RF. Efficacy of chlorhexidine in disinfecting dentinal tubules in vitro. Endod Dent Traumatol 9:243-8, 1993   DOI
25 Birkedal-Hansen H. Proteolytic remodellation of extracellular matrix. Current Opinion in Cell Biology 7:728-35, 1995   DOI   PUBMED   ScienceOn
26 Palosaari H, Wahlgren J, Larmas M, Ronka H, Sorsa T, Salo T, Tjaderhane L. The expression of MMP-8 in human odontoblasts and dental pulp cells is down-regulated by TGF-betal. J Dent Res 79:77-84, 2000   DOI   ScienceOn
27 Kurita-Ochiai T, Ochiai K, Ikeda T. Immunosuppressive effect induced by Actinobacillus actinomycetemcomitans: effect on immunoglobulin production and lymphokine synthesis. Oral Microbiol Immunol 7(6):338-43, 1992   DOI   ScienceOn
28 Ohara P, Torabinejad M, Kettering JD. Antibacterial effects of various endodontic irrigants on selected anaerobic bacteria. Endod Dent Traumatol 9:95-100, 1993   DOI
29 Siqueira Junior JF, Lopes HP. Mechanisms of antimicrobial activity of calcium hydroxide: a critical review. Int Endod J 32:361-9, 1999   DOI   PUBMED
30 Love RM, Enterococcus faecalis-a mechanism for its role in endodontic failure. Int Endod J 34:399-405, 2001   DOI   PUBMED   ScienceOn
31 Bystrom A, Claesson R, Sundquvist G. The antibacterial effect of camphorated paramonochlorophenol. camphorated phenol and calcium hydroxide in the treatment of infected root canals. Endod Dent Traumatol 1:170-5, 1985   DOI
32 Sundqvist G, Figdor D, Persson S, Sjogren U. Microbiologic analysis of the teeth with failed endodontic treatment and outcome of conservative retreatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endo 85:86-93, 1998   DOI   ScienceOn