Browse > Article
http://dx.doi.org/10.5395/JKACD.2004.29.5.479

The Effect of Treponema denticola immunoinhibitory protein on cytokine expression in T cells  

Lee Sang-Yup (Department of Conservative Dentistry, School of Dentistry, Seoul National University)
Shon Won-Jun (Department of Conservative Dentistry, School of Dentistry, Seoul National University)
Lee WooCheol (Department of Conservative Dentistry, School of Dentistry, Seoul National University)
Baek Seung-Ho (Department of Conservative Dentistry, School of Dentistry, Seoul National University)
Bae Kwang-Shik (Department of Conservative Dentistry, School of Dentistry, Seoul National University)
Lim SungSam (Department of Conservative Dentistry, School of Dentistry, Seoul National University)
Publication Information
Restorative Dentistry and Endodontics / v.29, no.5, 2004 , pp. 479-484 More about this Journal
Abstract
Immunoinhibitory protein extracted from sonicated Treponema denticola have been shown to suppress cell cycle progression of human lymphocytes. To study in detail about the effect of this microorganism on the function of lymphocytes. we investigated the levels of Interleukin-2 (IL-2) and Interleukin-4 (IL-4) production by T lymphocytes before and after the addition of $12.5{\;}\mu\textrm{g}/ml$ T. denticola sonicated extracts. In this study. levels of IL-2 and IL-4 produced from T cells pretreated with sonicated extracts were evaluated by using the quantitative sandwich enzyme immunoassay technique. In response to phytohemagglutinin (PHA) stimulation. T cell produced increased levels of IL-2 and IL-4. However. the expressions of both cytokines were significantly inhibited when PHA activated-T cells were pre-exposed to sonicated T. denticola extracts (p < 0.05). These findings suggest that the T. denticola sonicated extracts induced-immunosuppression in Th1 and Th2 cell functions could be a part of the pathogenic mechanism of the endodontic failure associated with this microorganism.
Keywords
Treponema denticola; Immunoinhibitory protein; T. denticola; Sonicated extracts; Interleukin-2; Interleukin-4;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Loesche W. The role of spirochetes in periodontal disease. Adv Dent Res 2:275-283, 1988   DOI   PUBMED
2 Shenker BJ, Listgarten MA, Taichman NS. Suppression of human lymphocyte responses by oral spirochetes: A monocyte-dependent phenomenon. J Immunol 132:2039-2045, 1984
3 Stashenko P. The role of immune cytokines in the pathogenesis of periapical lesions. Endod Dent Traumatol 6:89-96, 1990   DOI
4 Yoshida H, Jontell M, Sundqvist G, Bergenholtz G. Effect of sonicated material from Fusobacterium nucleatum on the functional capacity of accessory cells derived from dental pulp. Oral Microbiol Immunol 10:208-212, 1995   DOI   ScienceOn
5 Lee W, Pankoski L, Zekavat A, Shenker BJ. Treponema denticola imunoinhibitory protein induces irreversible G1 arrest in activated human lymphocytes. Oral Microbiol Immunol 19:144-149, 2004   DOI   ScienceOn
6 Gentry-Weeks CR, Karkhoff-Schweizer R, Pikis A, Estay M, Keith JM. Survival of Enterococcus faecalis in mouse peritoneal macrophages. Infect Immun 67:2160-2165, 1999   PUBMED
7 Okada H, Murakami S. Cytokine expression in periodontal health and disease. Crit Rev Oral Biol Med 9:248-66, 1998   DOI   ScienceOn
8 Siqueira JF, Rocas IN, Favieri A, Santos KRN. Detection of Treponema denticola in endodontics infections by 16S rRNA gene detected polymerase chain reaction. Oral Microbiol Immunol 15:335-337, 2000   DOI   ScienceOn
9 Thilo B, Baehni P, Holz J, Baume LJ. distribution des bacteries dans les parties coronaire et apicale de dents a pulpe necrosee. Rev Mens Suisse Odonto-Stomatol 93:335-350, 1983
10 Baumgartner JC, Khemaleelakul S, Xia T. Identification of spirochetes (Treponemes) in endodontic infections. J Endodon 29:794-797, 2003   DOI   ScienceOn
11 Smallwood E, Gharbia SE, Gulabivala K, Shah HN. Isolation and direct nucleic acid detection of oral spiro-chetes in root canal infections. Int Endo J 31:189-220, 1998   DOI   ScienceOn
12 Abbs AK, Murphy KM, Sher A. Functional diversity of helper T lymphocytes. Nature 383:787-793, 1996   DOI   ScienceOn
13 Listgarten MA. Colonization of subgingival areas by motile rods and spirochetes: clinical implication in host-parasite interaction in periodontal diseases. American Society of Microbiology. Washington D.C. p112, 1982
14 Nair PNR. Light and electron microscopic studies of root canal flora and periapical lesions. J Endodon 13:29-39, 1987   DOI   ScienceOn
15 Harris JI, Russell RRB, Curtis MA, Aduse-Opoku J, Taylor JJ. Molecular mediators of Porphyromonas gingivalis-induced T-cell apoptosis. Oral Microbiol Immunol 17:224-230, 2002   DOI   ScienceOn
16 Jung I, Choi B, Kum K, Roh B, Lee S, Lee C, Park D. Molecular epidermiology and association of putative pathogens in root canal infection. J Endodon 26:599-604, 2000   DOI   ScienceOn
17 Chan E, McLaughlin R. Taxonomy and virulence of oral spirochetes. Oral Microbiol Immunol 15:1-9, 2000   DOI   ScienceOn
18 Shenker BJ, Datar S. Fusobacterium nucleatum inhibits human T-cells by arresting cells in the mid G1 phase of the cell cycle. Infect Immun 63:4830-4836, 1995   PUBMED
19 Lee W, Lim S, Son H, Bae K. Sonicated Extract of Enterococcus faecalis induces irreversible cell cycle arrest in phytohemagglutinin-activated human lymphocytes. J Endodon 30:209-212, 2004   DOI   ScienceOn
20 Siqueira JF, Rocas IN. Treponema socranskii in primary endodontic infections as detected by nested PCR. J Endodon 29:244-247, 2003   DOI   ScienceOn
21 Siqueira JF, Rocas IN, Oliveira JCM, Santos KRN. Detection of putative oral pathogens in acute periradicular abscesses by 16S rDNA-directed polymerase chain reaction. J Endodon 27:164-167, 2001   DOI   ScienceOn
22 Kesavalu L, Walker S, Holt S, Crawley R, Eversole J. Virulence characteristics of oral treponemes in a murine model. Infect Immun 65:5096-5102, 1997   PUBMED