Browse > Article
http://dx.doi.org/10.5395/JKACD.2003.28.6.435

ANTIBACTERIAL EFFECT OF POLYPHOSPHATE ON ENDODONTOPATHIC BACTERIA  

Shin, Jeong-Hee (Department of Conservative Dentistry, Division of Dentistry, Graduate School of Kyung Hee University)
Park, Sang-Jin (Department of Conservative Dentistry, Division of Dentistry, Graduate School of Kyung Hee University)
Choi, Gi-Woon (Department of Conservative Dentistry, Division of Dentistry, Graduate School of Kyung Hee University)
Publication Information
Restorative Dentistry and Endodontics / v.28, no.6, 2003 , pp. 435-448 More about this Journal
Abstract
This study was performed to observe the antibacterial effect of polyphosphate (polyP) with various chain lengths (P3~P75) on virulent. invasive strains of P. gingivalis A7A1-28 and W50, and multidrug resistant E. faecalis ATCC29212. P. gingivalis strains were grown in brain-heart infusion broth (BHI) containing hemin and vitamin K with or without polyP. PolyP was added at the very beginning of the culture or during the exponential growth phase of the culture. Inhibition of the growth of P. gingivalis was determined by measuring the absorbancy at 540nm of the grown cells. Viable cell counts of the culture and release of intracellular nucleotide from P. gingivalis were measured. E. faecalis was grown in plain BHI with antibiotics alone or in combination with polyP(calgon: 0.1~1.0%) and the bacterial absorbancy was measured. The overall results suggest that polyP has a strong antibacterial effect on the growth of the virulent strains of P. gingivalis and the antibacterial activity of polyP seems largely bactericidal. accompanying bacteriolysis in which chelation phenomenon is not involved. Although polyP does not exert antibacterial activity against E. faecalis, it appears to increase antibacterial effect of erythromycin and tetracycline on the bacterium. Therefore, polyP alone or in combination with antibiotics may be developed as a candidate for the agent controlling oral infections including endodontic infection.
Keywords
Endodontopathic bacteria; Polyphosphate; Antibacterial effect; P. gingivalis; E. faecaliso; Calgon;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Haapasalo M: Bacteroides spp. in dental root canal infections. Endod Dent Treometot. 5:110, 1989
2 Rupf S, Eannengieeser S, Merte K, Pfister W, Sigusch B and Eschrich K: Comparison of profiles of key periodontal pathogens in periodontium and endodontium. Endod Dent Traumatol. 16:269 275, 2000   DOI
3 Griffee MB, Patterson SS, Miller CH, Kafrawy AH and Newton CW: The relationship of Bacteroides melanino genicus to symptoms associated with pulpal necrosis. Oral Surg Oral Med Oral Pethol. 50:457 461. 1980   DOI   ScienceOn
4 Yoshida M, Fukushima H, Yamamoto K, Ogawa K, Toda T and Sagawa H: Correlation between clinical symptoms and microorganisms isolated from root canals with periapical lesions. J Endod, 13:24 28, 1987   DOI   ScienceOn
5 Haapasalo M, Ranta H, Ranta K and Shah H: Black pigmented Bacteroides spp. in human apical periodontitis. Infect Immun. 53:149 153,1986   PUBMED
6 Mayrand D and Holt SC: Biology of assccharolytic black pigmented Bacteroides species. Microbiol Rev, 52: 134 152, 1988   PUBMED
7 Wingrove JA, DiScipio RG, Chen Z, Potempa J, Travis J and Hugli TE: Activation of complements C3 and C5 by a cysteine proteinase (Gingipain 1) from Porph yromonas (Bacteroides) gingivalis, J Biol Chem, 267:18902 18907, 1992   PUBMED
8 Lee JY, Sojar HT, Bedi GS and Genco RJ: Porphyromonas (Bacteroides) gingivalis fimbrillin:size, amino terminal sequence, and antigenic heterogeneity. Infect Immun. 59:383 9, 1991   PUBMED
9 Amano A, Nakagawa I. Kataoka K, Morisaki I and Hamada S: Distribution of Porphyromonas gingivalis strains with fimA genotypes in periodontitis patients. J Clin Microbiol. 37:1426 1430,1999   PUBMED
10 Katz J, Ward DC and Michalek SM.: Effect of host responses on the pathogenicity of strains of Porphyromonas gingivalis, Oral Microbiol Immunol, 11:309 318, 1996   DOI   ScienceOn
11 Sundqvist G. Figdor D. Persson S and Sjogren U: Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 85:86 93,1998   DOI   ScienceOn
12 Linden PK: Treatment options for vancomycin resistant enterococcal infections. Drugs, 62:425 441, 2002
13 Lima KC, Fava LR and Siqueira JF Jr.: Susceptibilities of Enterococcus faecalis biofilms to some antimicrobial medications. J Endod, 27:616 619, 2001   DOI   ScienceOn
14 Almyroudi A, Mackenzie D, McHugh S and Saunders WP: The effectiveness of various disinfectants used as endodontic intracanal medications: an in vitro study. J Endod, 28:163 167, 2002   DOI   ScienceOn
15 Haapasalo HK, Siren EK, walttmc TM, Orstavfk D and Haapasalo MP.: Inactivation of local root canal medicaments by dentine: an in vitro study. Int Endod J, 33:126 31, 2000   DOI   ScienceOn
16 Molander A, Reit C and Dahlen G: The antimicrobial effect of calcium hydroxide in root canals pretreated with 5% iodine potassium iodide. Endod Dent Traumatol. 15:205 9, 1999   DOI
17 Fuss Z, Charniaque O, Pilo R and Weiss E: Effect of various mixing ratios on antibacterial properties and hardness of endodontic sealers. J Endod, 26:519 522, 2000   DOI
18 박석범, 최기운, 최호영:Plyphosphate가 함유된 근관충전재가 구강세균의 성장에 미치느 영향, 대한치과보존학회지. 26: 141 152, 2001
19 Zaika LL and Kim AH: Effect of sodium polyphosphate on growth of Listeria monocytogenes, J Food Prot, 56:577 580, 1993   DOI
20 공희정, 최호영, 민병순, 박상진, 이진용, 최기운: 구강세균 Prevotella lntermedia의 성장에 따른 polyphosphate 의 영향에 관한 연구. 대한치과보존학회지, 23:550 560, 1998
21 최인식, 박병래, 김홍렬, 신세원, 최유진, 최영철. 이진용:Porphyromonas gingivalis에 대한 pclyphosphate의 향균 효과. 대한미생물학회지, 34:285 301, 1999
22 Socransky SS, Haffajee AD, Cugini MA, Smith C and Kent RLJr.: Microbial complexes in subgingival plaque. J Clin Periodontol, 25: 134 144, 1998   DOI   ScienceOn
23 Chow JW, Davidson A Sanford E 3rd and Zervos MJ: Superinfection with Enterococcus faecalis during quinupriatin/dalfopriatin therapy. Clin Infect Dis, 24:91 92,1997   DOI   ScienceOn
24 최성백, 박상진, 최기운, 최호영: Porphyromonas endodontalis에 대한 pclyphoephate의 항균기전에 관한 연구. 대한치과보존학회지. 25:561 574, 2000
25 김태진:Candida alhicans에 대한 polyphosphate 의 항균효과.경희대학교 석사논문. 2002
26 Post FJ and Krishnamurty GB, Flanagan MD: Influence of sodium hexametaphosphate on selected bacteria. Appl Microblol, 11:430 435,1963
27 Seymour RA and Heasman FA: Tetracyclines in the management of periodontal diseases. A review. J Clin Periodontol, 22:22 35, 1995   DOI   PUBMED   ScienceOn
28 Lee JY, Ryu DM, Shin and JW: Inorganic polyphoaphate promotes bone regeneration. Korean J Oral Anat, 23:219 235, 1999
29 Hamada, N, Watanabe K, Arai M, Hiramine H and Umemotc T: Cytokine production induced by a 67 kDa fimbrial protein from Porphyromonas gingivalis, Oral Microbiol Immunol. 17:197 200, 2002   DOI   ScienceOn
30 Shibata H and Morioka T: Antibacterial action of condensed phosphates on the bacterium Streptococcus mutans and experimental caries in the hamster. Arch Oral Blol, 27:809 816, 1982   DOI   ScienceOn
31 Andres MT, Chung WO, Roberts MC and Fierro JF: Antimicrobial susceptibilities of Porphyromonas gingivalls, Prevotella intermedia. and Prevotella nigrescensspp. isolated in Spain. Antimicrob Agents Chemother, 42:3022 3023, 1998
32 Haapasalo M: Black pigmented Gram negative anaer obes in endodontic infections. FEMS Immunol Med Microbiol, 6:213 218, 1993   DOI   ScienceOn
33 Miller WD: Microorganisms of the human mouth. S.S. White Dental Co. Philadelphia, 1890
34 Van Winkelhoff AJ, van Steenbergen TJM, Kippuw N and de Graaff J: Enzymatic characterization of oral and nonoral black pigmented Bacteroides species. Anton. Van Leeuwenh., 52:163 171, 1986   DOI   ScienceOn
35 Ding Y, Haapasalo M, Kerosuo E, Lounatmaa K, Kotiranta A and Sorsa T: Release and activation of human neutrophil matrix metallo and serine protemases during phagocytosis of Fusobacterium nucleatum, Porphyromonas gingivalis and Treponema denticola, J Clin Periodontol, 24:237 248, 1997   DOI   ScienceOn
36 Dahlen G and Moller AJR: Microbiology of endodontic infections. p,444 475. In Slots, J. and Taubman M.A. (ed.), Contemporary oral microbiology and immunology, Mosby Year Book Inc. St. Louis, 1992
37 Van Winkelhoff AJ, Carlee AW and de Graaff J:Bacteroides endodontalis and other black pigmented Bacteroides species in odontogenic abscesses. Infect Immun. 49: 494 497,1985   PUBMED
38 Hancock HH. 3rd., Sigurdsson A, Trope M and Moiseiwitsch J: Bacteria isolated after unsuccessful endodontic treatment in a North American population, Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 91:579 586, 2001   DOI   ScienceOn
39 Gomes BP, Ferraz CC, Vienna ME, Berber VB, Teixeira FB and Souza Filho FJ: In vitro antimicrobial activity of several concentrations of sodium hypochlorite and chlorhexidine gluconate in the elimination of Enterococcus faecalis. Int Endod J, 34:424 428, 2001   DOI   ScienceOn
40 Fuss Z, Weiss EI and Shalhav M: Antibacterial activity of calcium hydroxide containing endodontic sealers on Enterococcus faecalis in vitro. Int Endod J, 30:397 402, 1997
41 Niliue AM, Spencer SC and Simonson LG: Stimulation of in vitro growth of Treponema dentlcola by extraoel lular growth factors produced by Porphyromonas gingivalis, J Dent Res, 72:1027 1031. 1993   DOI   PUBMED   ScienceOn
42 Neiders ME, Chen PB, Suido H, Reynolds HS, Zambon JJ, Shlossman M and Genco RJ: Heterogeneity of virulence among strains of Bacteroides gingivalis . J Periodontal Res, 24:192 198, 1989   DOI   PUBMED
43 Ebersole JL, Kesavalu L, Schneider SL, Machen RL and Holt SC: Comparative virulence of periodon topathogena in a mouse abscess model. Oral Dis, 1:115 128,1995   DOI   PUBMED   ScienceOn
44 Rams TE, Feik D, Lisgarten MA and Slots J: Enterococci in human periodontitis. Oral Microbiol Immunol. 7:249 252, 1992   DOI   ScienceOn
45 Lee RM, Hartman PA, Stahr HM, Olson DG and Williams FD: Antibacterial mechanism of long chain polyphosphates in Staphylococcus aureus. J Food Prot, 57:289 294, 1994   DOI
46 Kornberg A, Narayama NR and Ault Riche D: Inorganic polvphosphate: a molecule of many functicns. Annu Rev Biochem, 68:89 125, 1999   DOI   ScienceOn
47 U. S. Department of Agriculture: Meat and poultry products: phosphates and sodium hydroxide. Federal Register Rule and Regulatlon 47:10779,1982
48 Ayres HM, Furr JR and Russell AD: Effect of permeahilizers on antibiotic sensitivity of Pseudomonas aeruginosa: Lett Appl Microbiol, 28:13 16, 1999   DOI   ScienceOn
49 강계숙: Mutans streptococci에 대한 polyphcsphate의 항균 효과. 경희대학교 박사논문.2001
50 Morrison D, Woodford N and Cookson B : Enterococci as emerging pathogens of humans. J Appl Microbiol, 83:S89 S99, 1997   DOI   ScienceOn
51 Baker PJ, Dixon M, Evans RT and Roopenian DC: Heterogeneity of Porphyromonas gingivalis strains in the induction of alveolar bone loss in mice. Oral Microbiol. Immunol, 15:27 32, 2000   DOI   ScienceOn
52 Ayres H, Furr JR and Russell AD: A rapid method of evaluating permeabilizing activity against Pseudomonas aeruginosa, Lett Appl Microbiol, 17: 149 151. 1993   DOI   ScienceOn
53 Jones ME, Gesu G, Ortisi G, Sahm DF, Critchley IA and Goglio A: Proficiency of Italian clinical laboratories in detecting reduced glycopeptide susceptibility in Enterococcus and Staphylococcus spp. using routine laboratory methodologies. Clin Microbiol Infect, 8:101 111, 2002   DOI   ScienceOn
54 Jagels MA, Travis J, Potempa J, Pike R and Hugli TE: Proteolytic inactivation of the leukocyte C5a receptor by proteinases derived from Porphyromonas gingivalis, Infect Immun. 64:1984 1991. 1996   PUBMED
55 Jett BD, Huycke MM and Gilmore MS: Virulence of enterococci. Clin Microbiol Rev, 7:462 478, 1994   DOI
56 Torabinejad M, Hong CU, Pitt Ford TR and Kettering JD: Antibacterial effets of some root end filling materials. J Endod, 21: 403 406, 1995   DOI   ScienceOn
57 Nakamura R, Hinode D, Terai H and Morioka M: Extracellualr enzymes of Porphyromonas (Bacteroides) gingivalis in relation to periodontal destruction. P.129 141, In S. Hamada, S.C. Holt, and J.R. McGhee (ed.), Periodontal diseases: pathogens and host immune responses, Quintessence Publishing Co.. Ltd.. Tokyo, 1991
58 Engstrom B: The significance of enterococci in root canal treatment. Odont Revy, 15:87 106, 1964
59 Love RM: Entercococus faecalis a mechanism for itsrole in endodontic failure. Int Endod J, 34:399 405, 2001   DOI   PUBMED   ScienceOn
60 Tanzer JM and Hageage GJJr.: Polyphosphate inhibition of growth of plaque formed by streptococci and diphtheroids implicated in oral disease. Infect Immun. 1:604 606,1970   PUBMED
61 Knabel SJ, Walker HW and Hartman PA: Inhibition of Aspergillus flavus and selected Gram positive bacteria by chelation of essential metal contains by polyphos phate. J Food Prot, 54:360 365, 1991   DOI
62 Yao ES, Lamont RJ, Leu SP and Weinberg A: Interbacterial binding among strains of pathogenic and commensal oral bacterial species. Oral Microbiol Immunol. 11:35 41. 1996   DOI   ScienceOn
63 Van Steenbergen TJM, Kastelein P,Touw JJA and de Graaff J: Virulence of black pigmented Bacteroides strains from periodontal pockets and other sites in experimentally induced skin lesions in mice. J Periodontal Res, 17:41 49, 1982   DOI   PUBMED
64 Genco CA, Cutler CW, Kapczynski D, Maloney K and Arnold RR: A novel mouse model to study the virulence of and host response to Porphyromonas (Bacteroides) gtrigivalis, Infect Immun. 59:1255 1263, 1991   PUBMED
65 Whlin YB and Holm AK: Changes in the oral microflora in patients with acute leukemia and related disorders during the period of induction therapy. Oral Surg Oral Med Oral Pathol, 65:411 417, 1988   DOI   ScienceOn
66 Dahlen G, Semuelsson W, Molander A and Reit C: Identification and antimicrobial susceptibility of enterccccci isolated from the root canal. Oral Microbiol Immunol, 15:309 312, 2000   DOI   ScienceOn
67 Chen PB, Neiders ME, Millar SJ, Reynolds HS and Zambon JJ: Effect of immunization on experimental Bacteroides gingivalis infection in a murine model. Infect Immun. 55:2534 2537, 1987   PUBMED
68 Vemillo AT and Rifkin BR: Effects of tetracyclines on bone metabolism. Adv Dent Res, 12:56 62, 1998   DOI   ScienceOn
69 Frandsen EVG: Hydrolysis of immunoglobulins. p.219 226. In H,N. Shah, D. Mayrand. and R.J. Genco (ed.), Biology of the species Porphyromonas gingivalis, CRC Press, Boca Raton, 1993
70 Siren EK, Haapasalo MP, Ranta K Salmi P and Kerosuo EN: Microbiological findings and clinical treatment procedures in endodontic cases selected for microbiological investigation. Int Endod J, 30:91 95, 1997   DOI   ScienceOn
71 Ellwood DC and Tempest DW: Effects of environment on bacterial wall content and composition. Adv Microb Physiol, 7:83 117, 1972   DOI
72 0rstavik D and Haapasalo M: Disinfection by endodcntic irriganta and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol. 6:142 149, 1990   DOI
73 Shalhav M, Fuss Z and Weiss EI: In vitro antibacterial activity of a glass ionomer endodontic sealer. J Endod, 23:616 619,1997   DOI   ScienceOn
74 Sundqvist G: Bacteriological studies of necrotic dental pulps. Thesis. Umea University, Odontology, Diss ertation No.7, 1976 (cited from the reference No.4)
75 Sundqvist G, Johansson E and Sjogren U: Prevalence of black pigmented Bacteroides species in root canal infections. J Endod, 15:13 19, 1989   DOI   ScienceOn
76 Socransky SS and Haffajee AD: Microbial mechanisms in the pathogenesis of destructive periodontal diseases: a critical assessment. J Periodont Res, 26:195 212,1991   DOI   PUBMED
77 Henrici AT and Hartzell T.B.: The bacteriology of vital pulp. Res J, 1:419 422, 1919   DOI
78 Tai H, Kobayashi T and Hara K: Changes in complement and immunoglobulin G receptor expreassion on neutrophile associated with Porphyromonas gingivalis induced inhibition of phagocytosis. Infect Immun. 61:3533 3535, 1993   PUBMED
79 Kobayashi T, Hayashi A Yoshikawa R, Okuda K and Hara K: The microbial flora from root canals and periodontal pockets of non vital teeth associated with advanced periodontitis. Int Endod J, 23:100 106, 1990   DOI   PUBMED
80 Economides N, Kotsaki Kovatsi VP, Poulopouloa A, Kolokuris I. Rozos G and Shore R: Experimental study of the biocompatibility of four root canal sealers and their influence on the zinc and calcium content of several tissues. J Endod, 21:122 127,1995   DOI   ScienceOn
81 Chung WO, Gabany J, Persson GR and Roberts MC: Distribution of erm(F) and tet(Q) genes in 4 oral bacterial species and genotypic variation between resistant and susceptible isolates. J Clin Periodontol, 29:152 158, 2002   DOI   ScienceOn
82 Rccae IN, Siqueira JFJr, Santos KR and Coelho AM: 'Red complex' (Bacteroides forsythus, Porphyromones gingivalis, and Treponema denticola) in endodontic infections: a molecular approach. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 91:468-471, 2001   DOI   ScienceOn
83 Evans RT, Klausen B, Ramamurthy NS, Golub LM, Sfintescu C and Genco RJ.: Periodontopathic potential of two strains of Porphyromonas gingivalis in gnotobiotic rat. Arch Oral Biol, 37:813 819, 1992   DOI   ScienceOn
84 Kulaev IS: Biochemistry of inorganic polyphosphates, Rev Physiol Biochem Pharmacol. 73:131 158, 1975   DOI