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THE EFFECT OF PERMANENT MAGNET CONNECTING WITH DENTAL IMPLANT ON BONE EXTRACELLULAR MATRIX FORMATION  

Won, In-Jae (Department of Prosthodontics, Division of Dentistry, Graduate School, Kyung Hee University)
Baik, Jin (Department of Prosthodontics, Division of Dentistry, Graduate School, Kyung Hee University)
Kwon, Kung-Rock (Department of Prosthodontics, Division of Dentistry, Graduate School, Kyung Hee University)
Lee, Sung-Bok (Department of Prosthodontics, Division of Dentistry, Graduate School, Kyung Hee University)
Publication Information
The Journal of Korean Academy of Prosthodontics / v.44, no.5, 2006 , pp. 574-583 More about this Journal
Abstract
Statement of problem : The use of permanent magnetics is increasing in implant dentistry. Purpose : This study is to know the effect of permanent magnetics on bone matrix formation of osteoblasts. Materials and methods : The konus abutment-shaped permanent magnetics were connected to the implant fixture, and placed on the culture plate. The osteoblast-like cell Mc3T3E1 were used for cell culture. As the control group, the implants were connected to titanium healing caps, and cultured in the same conditions of experimental group. After 3. 7, 14 days, cells were cultured, and we measured and compared the amount of collagen type I, osteocalcin, which is bone matrix protein by Western immunoblotting analysis. Results: As a result of Western immunoblotting analysis for estimating the amount of bone extracellular matrix, there was no difference between osteoblast of the experimental group and the control group during 3 and 7day-osteoblast culturing. However when cells were cultured for 14days, the amount of bone extracellular matrix was increased, on the experimental group. Conclusion: From these results, magnetic field of permanent magnetics might have effect on bone formation of osteoblast, especially at initial stage of implant placement. Therefore, their clinical application for implant or bone graft could be possible.
Keywords
Bone extracellular matrix; Permanent magnet; Collagen type I; Osteocalcin;
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1 Yasuda I, Fukuda E. On the piezoelectric effect of bone. J Physiol Soc Japan 1957:12:121-128
2 Korenstein R, Somjen D, Fischer H, Binderman I. Capacitative pulsed electric stimulation of bone cell. Induction of cyclic-AMP and DNA synthesis. Biochem Biophys Acta 1984:803:302-307   DOI   ScienceOn
3 Bodamyali T, Bhatt B, Hughes F,. Winrow VR, Kanczler JM, Abbott J, Blake DR, Stevens CR. Pulsed electricmagnetic fields simultaneously induce osteogenesis and upregulated transcription of bone morphogenic proteins 2 and 4 in rat osteoblast in vitro. Biochem Biophys Res Commun 1998:250:458-461   DOI   ScienceOn
4 McDonald F. Effect of static magnetic fields on osteoblasts and fibroblasts in vitro. Bioelectromagnetics 1993:14:187-196   DOI   ScienceOn
5 Bassett CA, Pawluk RJ, Pilla AA. Augmentation of bone repair by inductively coupled electromagnetic fields. Science 1974:184:575-577   DOI   ScienceOn
6 Friedenberg ZG, Robert PG, Didizian NH, Brighton CT. Stimulation of fracture healing by direct current in the rabbit fibula. J Bone Joint Surg 1971:53:1400-1408   DOI
7 Stan S, Sansen W, Muline JC. Experimental study on the electrical impendance of bone and the effect of direct current on the healing of fracture. Clin Orthop 1976:120:264-267
8 Yan QC, Tomita N, Ikada Y. Effect of static magnetic field on bone formation of rat femur. Med Eng Phys 1998:20:397-402   DOI   ScienceOn
9 Bassett CA. Pawluk RJ. Noninvasive method for stimulating osteogenesis. J Biomed Mater Res 1975:9:371-374   DOI   ScienceOn
10 Matsunaga S, Sakou T, Yoshikuni N, et al. Intramedullary callus induced by weak direct current stimulation: Serial changes in the alkaline phosphatase activity at the site of electricity induced callus formation. J Japan Bioelect Res Soc 1988:2:67-71
11 Yasuda I. Piezoelectricity of living bone. J Kyoto Pref Univ Med 1953:53:325
12 Harris H. The human alkaline phosphatase: What we know and what we don't know. Clin Chim Acta 1990:186:133-150   DOI   ScienceOn
13 Canalis E, McCarthy T, Centrella M. Growth factors and the regulation of bone remodeling. J Clin Invest 1988:81:277-281   DOI   ScienceOn
14 Xu S, Tomita N, Ohata R, Yan Q. Static magnetic field effects on bone formation of rats with an ischemic bone model. Biomed Mater Eng 2001:11:257-263
15 Stan S, Sansen W, Muline JC. Experimental study on the electrical impendance of bone and the effect of direct current on the healing of fracture. Clin Orthop 1976:120:264-267
16 Inoue S, Ohashi S, Kajikawa K et al. The effects of electric stimulation on the differentiation to the bone. Orthop Res Sci 1980:7:501-507
17 Basset CAL, Mitchell SN, Norton L, Pilla AA. A nonoperative salvage of surgically resistant pseudarthoses and nonunions by pulsing electromagnetic fields. a preliminary report. Clin Orthop 1977:1245:128143
18 Mareke Hartig, Ulrich Joos, Hans-Peter Wiesmann. Capacitively coupled electric fields accelerate proliferation of osteoblastlike primary cells and increase bone extracellular matrix formation in vitro. Eur Biophys J 2000:29:499-506   DOI   ScienceOn
19 McCarthy T, Centrella M, Canalis E. Regulatory effects of insulin-like growth factor I and II on bone collagen synthesis in rat calvarial cultures. Endocrinology, 1989:124:301-309   DOI   ScienceOn
20 Yasuda I, Nagayama H, Kato T, et al. Fundamental problems in the theatment of fracture. J Kyoto Med Soc 1953:4:395406
21 Sato K, Yanaguchi H, Miyamoto H, Kinouchi Y. Growth of human cultured cells exposed to a non-homogenous atatic magnetic field generated by Sm-Co magnets. Biochim Biophys Acta 1992:1136:231-238   DOI   ScienceOn
22 Cho YW, Lee SB, Chio BB. The effect of magnetism(neodymium magnet) on activity of osteoblast. J Korean Academy of Stomatognathic Function and Occlusion 2003:19:185-194
23 Fitzsimmons RJ, Farley JR, Adey WR, Baylink DJ. Frequency dependence of increased cell proliferation, in vitro, in exposures to a low-amplitude, low frequency electro field: evidence for dependence on increase mitogen activity released into culture medium. J Cell Physiol 1989:139:586-591   DOI
24 Esformes I, Kummer FJ, Livelli TJ. Biological effects of magnetic fields generated with CoSm magnets. Bull Hosp Jt Orthop Inst 1981:41:81-87
25 Hossenlopp P, Seurin D, Segovia-Qinson B. Hardouin S, Binoux M. Analysis of serum insulin-like growth factor binding proteins using Western blotting:Use of the method for titration of the binding proteins and competitive binding proteins and competitive binding studies. Anal Biochem, 1986:154:138-143   DOI   ScienceOn
26 Kawata T, Hirota K, Sumitani K. A new orthodontic force system of magneitc brakets. Am J Orthod Dentofacial Orthop 1987:92:241-248   DOI   ScienceOn
27 Brighton CT, Pollack SR. Treatment of recalitrant nonunion with a capacitively coupled electric field. J Bone Joint Surg 1985:67:577-585   DOI
28 Cieszynski Y. Studies on the regeneration of ossal tissue. II. Treatment of bone fracture in experimental animals with electric energy. Arch Immunol Ther Exp 1963:11:199-217
29 Lee SM, Lee SB, Chio BB. Effect of magnetism(neodymiun magnet) on growth factor receptors of osteoblast. J Korean Academy of Stomatognathic Function and Occlusion 2003:19:87-96
30 Jacobs JD, Norton LA. Electrical stimulation of osteogenesis in pathological osseos defects. J Periodontal 1976:47:311319
31 Centrella M, McCarthy T, Canalis E, Cyclic AMP induces insulin-like growth factor I synthesis in osteoblast-enriched cultures. J Biol Chern 1989:264:18268-18271