Browse > Article

THE EVALUATION OF CYTOTOXICITY AND BIOCOMPATIBILITY OF TI-TA-NB-BASE ALLOY  

Cui De-Zhe (Department of Prosthodontics, College of Dentistry, Chonnam National University)
Vang Mong-Sook (Department of Prosthodontics, College of Dentistry, Chonnam National University)
Yoon Taek-Rin (Department of Orthopedics, College of Medicine, Chonnam National University)
Publication Information
The Journal of Korean Academy of Prosthodontics / v.44, no.2, 2006 , pp. 250-263 More about this Journal
Abstract
Statement of problem: Ti-alloy has been used widely since it was produced in the United States in 1947 because it has high biocompatibility and anticorrosive characteristics. Purpose: The pure titanium, however, was used limitedly due to insufficient mechanical charateristics and difficult manufacturing process. Our previous study was focused on the development of a new titanium alloy. In the previous study we found that the Ti-Ta-Nb alloy had better mechanical characteristics and similar anticorrosive characteristics to Ti-6Al-4V Material and methods: In this study, the cytotoxicity of the Ti-Ta-Nb alloy was evaluated by MTT assay using MSCs(Mesenchaimal stem cells) and L929 cells(fibroblast cell line). The biocompatibility of the Ti-Ta-Nb alloy was performed by inserting the alloy into the femur of the rabbits and observing the radiological and histological changes surrounding the alloy implant. Results: 1. In the cytotoxicity test using MSCs, the 60% survival rate was observed in pure titanium, 84% in Ti-6Al-4V alloy and 95% in Ti-10Ta-10Nb alloy. 2. In the animal study, the serial follow-up of the radiographs showed no separation or migration revealing gradual bone ingrowth surrounding the implants. Similar radiographic results were obtained among three implant groups pure titanium, Ti-6Al-4V alloy and Ti-10Ta-10Nb alloy. 3. In the histologic examination of the bone block containing the implants. the bone ingrowth was prominent around the implants with the lapse of time. There was no signs of any tissue rejection, degeneration, or inflammation. Active bone ingrowth was observed around the implants. In the comparison of the three groups, the rate of bone ingrowth was better in the Ti-10Ta-10Nb alloy group than those in pure titanium group or Ti-6Al-4V alloy group. In conclusion, Ti-10Ta-10Nb alloy revealed better biocompatibility in survival rate of the cells and bone ingrowth around the implants. Therefore we believe a newly developed Ti-10Ta-10Nb alloy can replace currently used Ti-6Al-4V alloy to increase biocompatibility and to decrease side effects. Conclusion: In conclusion, Ti-10Ta-10Nb alloy revealed better biocompatibility in survival rate of the cells and bone ingrowth around the implants. Therefore we believe a newly developed Ti-10Ta-10Nb alloy can replace currently used Ti-6Al-4V alloy to increase biocompatibility and to decrease side effects.
Keywords
Ti-Ta-Nb-base Alloy; Biocompatibility; Cytotoxicity; Evaluation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Branemark PI. Osseointegration and its experimental background. J Prosthet Dent 1983 ;50: 399-410   DOI   ScienceOn
2 Ahmed T, Long M, Silvestri J. A New Low Modulus, Biocompatible Titanium Alloy. Titanium 95 science and technology 1995: 2:1760-1767
3 ITO Y, ITO A. et al. New Titaniun Alloys for Medical Implants. Titanium 95 science and technology 1995: 2: 1776-2065
4 Helgeland K, Leirskar J . Silicate cement in a cell culture system. Scand J Dent Res 1973:81(3) :251-259
5 Hanks CT, Anderson M, Craig RG. Cytotoxiceffects of dental cements on two cell culture system. J Oral Pathol 1981:10:101-102   DOI
6 Castleman LG, Motzkin SM, et al. Biocompatibility of ni-tinal alloy as an implant material. J Bio-med Mat Res 1988:10:695-97
7 Santavirta S, Garistina A. Konttinen VT. Cemented versus cementless hip arhroplasty: Areview of prosthetic biocompatibility. Acta Orthop Scand 1992:63:225-232   DOI   ScienceOn
8 Tyras MJ. A method for the in vitro toxicity testing of dental resorative materials. J Dent Res 1977:56:1285   DOI   ScienceOn
9 Kasemo B. Biocompatibility of titanium implants: Surface science aspects. J Prosthet Dent 1983:49:832-837   DOI   ScienceOn
10 Gross DM. Biocompatibility The interaction of biomaterials and host response. J Dent Educ 1988:52:798-803
11 Petrunko AN, Anokhin VM. Titanium in Medicine, Food Industry and Ecology. Titanium 95 science and technology 1995: 2:1816-1767
12 Rahal MD, Branemark P-I. Response of bone marrow to titanium implants: Osseointegration and the establishment of a bone marrow-titanium interface in mice. Int J Oral Maxillofas implants 1993:8:573-579
13 Ducheyne P. Titanium and calcium phosphate ceramic dental implants, surfaces, coatings and interfaces. J Oral implantal 1988:14:325-340
14 Smith DE, Zarb GA. Critieria for success of osseointegated endosseous implants. J Prosthet Dent 1989:62:567-577   DOI   ScienceOn
15 Lee HJ, Lee SC, Huh WS, et al. A study on mechanical properties and cytotoxicity of the new titanium alloys for implant material. Korean Academy of implant Dentalstry 1999: 18(1): 10-22
16 Davidson JA, Mishra AK. Kovacs P. New surface-hardened. low-modules. corrosionresistant Ti-13Zr-13Nb alloy for total hip arthroplasty. Biomed Eng 1994:114:231-243
17 Qingzhi WU, Zuochen LI. The Effect of Adding Elements on Corrosion Resistance of Ti-Ta Alloy. Titanium 95 science and technology 1995: 3: 2061-2067
18 Deporter DA. Watson PA. Pilliar RM, et al. Ahistological assessment of the initial hea-ling response adjacent to porous-surfaced, titanium alloy dental implants in dogs. J Den Res 1986:65(8):1064-1070   DOI   ScienceOn
19 Bordji K, Jouzeau JY. et al. Cytocompatibility of Ti-6Al-4V and Ti-5Al-2.5Fe alloys according to three surface treatments. using human fibroblasts and osteoblasts. Biomaterials 1996: 17(9) :929-940   DOI   ScienceOn
20 Albrektsson T. Osseointegrated titaniun implants. Acta Orthop Scand 1981:52:155-170   DOI   ScienceOn
21 Albrektsson T, Dahl E, Enbom L. Osseointegraed oral implants: A Swedish multicenter study of 8139 consecutively inserted Nobelpharma implants. J Periodontal 1988:59:289-296
22 Adell R, Lekholm U, Braenmark PI. A 15 year of osseointegrated implants in the treatment of the edentulous jaw. Int J Oral Surg 1981; 10: 387-416   DOI
23 Piattelli A. Trisi P, Emanuelli M. Bone reactions to hydroxyapatite-coated dental implants in humans: Histologic study using SEM, light microscopy and laser scanning microscody. Int J Oral Maxilofac implants 1993:8:69-74
24 ISO 10993-5, 1997 : Biological evaluation of medical devices part 5 : Tests for cytotoxicity : in vitro methods
25 Landsberg JP, McDonald B, Watt F. Absence of aluminium in neuritic plaque cores in Alzheimers disease. Nature 1992:360: 65-68   DOI   ScienceOn
26 Shayne CG. Safety evaluation of medical devices. now York: Marcel Dekker, 1997
27 Farrar G et al. Defective gallium-transferrin binding in Alzheimer disease and Down syndrome: possible mechanism for accumulation of aluminium in brain. Lancet 1990: 335:747-750   DOI   ScienceOn