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ADSORPTION OF HUMAN RECOMBINANT FIBRONECTIN TO TITANIUM IN VITRO  

Kim H.J. (Department of Prosthodontics, College of Dentistry, Seoul National University)
Chang I.T. (Department of Prosthodontics, College of Dentistry, Seoul National University)
Koak J.Y. (Department of Prosthodontics, College of Dentistry, Seoul National University)
Heo S.J. (Department of Prosthodontics, College of Dentistry, Seoul National University)
Yim S.A. (Department of Prosthodontic dentistry, Samsung Medical Center, School of Medicine, Sungkyunkwan University)
Jang J.H. (Intellectual Biointerface Engineering Center, Seoul National University College of Dentistry)
Publication Information
The Journal of Korean Academy of Prosthodontics / v.42, no.3, 2004 , pp. 301-306 More about this Journal
Abstract
Statement of problem. Fibronectin mediates its biological effects by binding to integrins on cell membranes through a consensus site including the Arg-Gly-Asp (RGD) sequence within tenth type III module. Purpose. The purpose of our study was to investigate the adsorption affinity of human recombinant fibronectin peptide (hFNIII 9-10) to titanium and to investigate the effect of the surrounding ionic composition on the adsorption process. Material and methods. As for evaluating the affinity of hFNIII 9-10 to Ti, titanium disks were incubated in 40, 80 and $120{\mu}g/ml$ hFNIII 9-10 solution at $37^{\circ}C$ overnight, repectively. As for evaluating the effect of surrounding ionic concentration, hFNIII 9-10 was dissolved in distilled water, phosphate buffered saline and RPMI 1640. Optical density (O.D.) was measured in ELISA reader. Results. The results were as follows; 1. The adsorption of hFNIII 9-10 showed significantly highest mean optical density (O.D.) value in $80{\mu}g/ml$. 2. The difference of ionic composition in DW, PBS and RPMI did not influence the adsorption amount of hFNIII 9-10.
Keywords
Fibronectin; Titanium;
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  • Reference
1 Kasemo B. Biocompatibility of titanium implants: Surface science aspects. J Prosthet Dent 1983;49:832-837   DOI   ScienceOn
2 Degan IL, Stetsula VI. Consolidation of bone fragments in a constant magnetic field. Ortop Tracmatol Protez 1971;32:45-48
3 Tsai CC, Dollar ML, Constationescu A, Kulkarni PV, Eberhart RC. Performance evaluation of hydoxylated and acylated silicone rubber coatings. Asaio Trans 1991;37:M192-M193   PUBMED
4 Sundgren JE, Bodo P, Ivarsson B, Lundstrom I. Adsorption of fibronigen on titanium and gold surfaces studied by ESCA and ellipsometery. J Colloid Interface Sci 1986;113:530-543   DOI   ScienceOn
5 Klinger A, Steinberg D, Kohavi D, Sela MN. Mechanism of adsorption of human albumin to titanium in vitro. J Biomed Mater Res 1997;36:387-392   DOI   ScienceOn
6 Clark EA, Brugge JS. Integrins and signal transduction pathways: the road taken. Science 1995;268:233-239   DOI
7 Cuypers PA, Hermens WT, Hemker HC. Ellipsometry as a tool to study protein films at liquid- solid interfaces. Anal Biochem 1978;84:56-57   DOI   ScienceOn
8 Freedman H. Magnetic counter-influence in full lower denture retention. Dental digest 1941;47:98-503
9 Collins JI, Embery G. Adsorption of glycosaminoglycans to commercially pure titanium. Biomaterials 1992;13:548-552   DOI   ScienceOn
10 Keogh JR, Velander FF, Eaton JW. Albumin binding surfaces for implantable devices. J Biomed Mater Res 1992;26:441-456   DOI
11 Sutherland DS, Forshow PD, Allen GC, Brown IT, Williams KR. Surface analysis of titanium implants. Biomaterials 1993;14:893-899   DOI   ScienceOn
12 Nygren H, Tengvall P, Lundstrom I. The initial reactions of Ti$O_2$ with blood. J Biomed Mater Res 1997;34:487-492   DOI   ScienceOn
13 Bruce GK, Howlett CR, Huckstep RL. Effect of a static magnetic field on fracture healing in a rabbit radius. Preliminary results. Clinical orthopaedics and related research 1987;222:300-306   PUBMED
14 Ruoslahti E, Pierschbacher MD. Mew perspectives in cell adhesion: RGD and integrins. Science 1987;238:491-497   DOI
15 Singer II, Kawka DW, Scott S, Mumford RA, Lark MW. The fibronectin cell attachment sequence Arg-Gly-Asp-Ser promotes focal contact formation during early fibroblast attachment and spreading. J Cell Biol 1987;104:573-584   DOI
16 Gomboltz WR, Wang GH, Horbett TA, Hoffman AS. Protein adsorption to poly(ethylene oxide) surfaces. J Biomed Mater Res 1991;25:1547-1562   DOI
17 MacDonald DE, Deo N, Markovic B, Stranick M, Somasundaran P. Adsorption and dissolution behavior of human plasma fibronectin on thermally and chemically modified titanium dioxide particles. Biomaterials 2002;23:1269-1279   DOI   ScienceOn
18 MacDonald DE, Markovic B, Boskey AL, Somasundaran P. Physico-chemical properties of human plasma fibronectin binding to well characterized titanium dioxide. Colloids Surf 1998;B11:131-139
19 Goldstin AS, DiMilla PA. Effect of adsorbed fibronectin concentration on cell adhesion and deformation under shear on hydrophobic surfaces. J Biomed mater Res 2002;59:665-675   DOI   ScienceOn
20 Tsai CC, Huo HH, Kulkarni PV, Eberhart RC. Biocompatible coatings with high albumin affinity. Asaio Trans 1990;36:M307-M310   PUBMED
21 Jang JH, Ku Y, Chung CP, Heo SJ. Enhanced fibronectin-mediated cell adhesion of human osteoblast by fibroblast growth factor, FGF-2. Biotechnology letters 2002;24:1659-1663   DOI   ScienceOn