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http://dx.doi.org/10.5392/JKCA.2021.21.04.678

Effect of Styrene on Hepatic Activities of Antioxidant Enzymes in Rats  

Lee, Jong-Ryol (백석문화대학교 보건행정과)
Kim, Dong Hun (경북도립대학교 응급구조과)
Lee, Sang-Min (경북도립대학교 응급구조과)
Publication Information
The Journal of the Korea Contents Association / v.21, no.4, 2021 , pp. 678-687 More about this Journal
Abstract
Styrene is a commercially important chemical used mainly in the production of raw materials and plastics. To determine the effect of styrene on hepatic activities of antioxidant enzymes, styrene was treated to Sprague-Dawley rats at 50 mg/kg, 200 mg/kg and 400 mg/kg (i.p) twice a day for 4 days. There were determined the significantly increased activities of serum AST (aspartate aminotransferase), ALT (alanine aminotransferse), and the increased content of MDA (malondialdehyde) at the dose of 400 mg/kg compared to the control. The hepatic activities of XO (xanthine oxidase) and CYPdAH (cytochrome P450 dependant aniline oxidase) in the dose of 400 mg/kg compared to the dose of 200 mg/kg were more increased, which means the excessive ROS (reactive oxygen species)s were produced during Phase I. In addition, significantly decreased were rates of the hepatic activities of GPx (glutathione peroxidase), CAT (catalase), SOD (superoxide dismutase) and GST (glutathione S-transferase) at the dose of 400 mg/kg compared to the control. And, the group at the dose of 400 mg/kg showed more significantly decreased GSH (glutathione) level than the group at the dose of 200 mg/kg. The decrease in GSH could ascribe to the toxic metabolites of styrene, such as styrene oxide. In conclusion, these results indicate that the excessive ROSs and the toxic metabolites of styrene may result in the hepatotoxicity, and be related to their imbalanced activities for antioxidant enzymes.
Keywords
Styrene; Reactive Oxygen Species; Antioxidant Enzymes; Hepatotoxicity;
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