Browse > Article
http://dx.doi.org/10.5392/JKCA.2011.11.2.341

The Effect of Electroacupuncture on Reactive Gliosis Expressing GFAP in Rat with Transient Global Cerebral Ischemia  

Cho, Mi-Suk (나사렛대학교 물리치료학과)
Publication Information
The Journal of the Korea Contents Association / v.11, no.2, 2011 , pp. 341-352 More about this Journal
Abstract
The purpose of this study was carried out to investigate the effect of electroacupuncture on reactive gliosis expressing GFAP in rat with transient global cerebral ischemia. Subjects were randomly divided into two groups, a control group and a electroacupuncture group on ST36, LI11 and SP9 with 2 Hz and 1 mA. The rats were sacrificed on 1, 3 and 7 days after transient cerebral ischemia using ligation of left common carotid artery. After making brain slide sections, they were immunostained with GFAP antisera(1:2,500). The results were as follows: The numbers of astrocytes of electroacupuncture group were decreased than those of control group at every 1, 2 and 7 days. Especially, the numbers of astrocytes at 3 days(p<0.01) and 8 days(p<0.05) were different statistically. And astrocytes had resting, hypertrophic and moving types on cerebral cortex. The decrease of numbers of astrocytes expressing GFAP showed that electroacupuncture could localise and minimize the brain damage by transient cerebral ischemia and cause brain cell plasticity.
Keywords
Electroacupuncture; Reactive Gliosis; Astrocyte; GFAP;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 S. Ueda, X. F. Gu, and P. M. Whitaker-Azmitia, Neuro-glial neurotrophic interaction in the S-100 beta retarded mutant mouse. Brain Res. Vol633, No.1-2, pp.275-283, 1994.   DOI   ScienceOn
2 J. E. Rice, 3rd, R. C. Vannucci, and J. B. Brierley, The influence of immaturity on hypoxic-ischemic brain damage in the rat. Ann Neurol. Vol.9, No.2, pp.131-141, 1981.   DOI   ScienceOn
3 J. Ong, J. M. Plane, and J. M. Parent, Hypoxicischemic injury stimulates subventricular zone proliferation and neurogenesis in the neonatal rat. Pediatr Res. Vol.58, No.3, pp.600-606, 2005.   DOI   ScienceOn
4 S. Ueda, X. F. Gu, and P. M. Whitaker-Azmitia, Neuro-glial neurotrophic interaction in the S-100 beta retarded mutant mouse. Brain Res, Vol.633, No.1-2, pp.275-283, 1994.   DOI   ScienceOn
5 신홍기, 박동석, 이서은, 전침자극이 흰쥐후각세포의 유해반응에 미치는 효과의 특성. 대한침구학회지, Vol.20, No.3, pp.117-130, 2003.
6 M. Culcasi, M. Lafon-Cazal, and S. Pietri, Glutamate receptors induce a burst of superoxide via activation of nitric oxide synthase in arginine-depleted neurons. J Biol Chem. Vol.269, No.17, pp.12589-12593, 1994.
7 U. Missler and M. Wiesmann, Measurement of S-100 protein in human blood and cerebrospinal fluid: analytical method and preliminary clinical results. Eur J Clin Chem Clin Biochem. Vol.33, No.10, pp.743-748, 1995.
8 S. Sun, M. Cha, M. Han, T. T. Li, Evidence for suppression of electroacupuncture on spinal glial activation and behavioral hypersensitivity in a rat model of monoarthritis. Brain Res. Bul. Vol.75, pp.83-93, 2008.   DOI   ScienceOn
9 Y. H. Chubg, C. M. Shin, and M. J. Kim, Enhanced expression of L-type Ca2+ channels in reactive astrocytes after ischemic injury in rats. Neurosci Let. Vol.302, pp.93-96, 2001.   DOI   ScienceOn
10 C. Du, R. Hu, and C. A. Csernansky, Very delayed infarction after mild focal cerebral ischemia: a role for apoptosis? J Cereb Blood Flow Metab, Vol.16, No.2, pp.195-201, 1996.   DOI
11 V. Biran, L. M. Joly, and A. Heron, Glial activation in white matter following ischemia in the neonatal P7 rat brain. Exp Neurol. Vol.199, No.1, pp.103-12, 2006.   DOI   ScienceOn
12 C. C. Chao, S. Hu, and W. S. Sheng, Cytokine-stimulated astrocytes damage human neurons via a nitric oxide mechanism. Glia, Vol.16, No.3, pp.276-284, 1996.   DOI
13 S. O. Suzuki and J. E. Goldman, Multiple cell populations in the early postnatal subventricular zone take distinct migratory pathways: a dynamic study of glial and neuronal progenitor migration, J. Neurosci. Vol.23, No.10, pp.4240-4250, 2003.
14 R. S. Cameron and P. Rakic, Glial cell lineage in the cerebral cortex: a review and synthesis. Glia. Vol.4, No.2, pp.124-137, 1991.   DOI
15 M. Kalman and B. M. Ajtai, A comparison of intermediate filament markers for presumptive astroglia in the developing rat neocortex: immunostaining against nestin reveals more detail, than GFAP or vimentin. Int J Dev Neurosci. Vol.19, No.1, pp.101-108, 2001.   DOI   ScienceOn
16 T. Voigt. Development of glial cells in the cerebral wall of ferrets: direct tracing of their transformation from radial glia into astrocytes. J Comp Neurol. Vol.289, No.1, pp.74-88, 1989.   DOI   ScienceOn
17 S. Ueda and X. F. Gu, Whitaker-Azmitia PM et al. Neuro-glial neurotrophic interaction in the S-100 beta retarded mutant mouse. Brain Res. Vol.633(1-2), pp.275-283, 1994.   DOI   ScienceOn
18 A. Yokoyama, A. Sakamoto, and K. Kameda, NG2 proteoglycan-expressing microglia as multipotent neural progenitors in normal and pathologic brains. Glia. Vol.53, No.7, pp.754-768, 2006.   DOI   ScienceOn
19 신홍기, 이경희, 박동석. 전침자극이 만성통증을 억제하는 아드레날린성 기전에 대한 연구, 대한한의학회지, Vol.25, No.3, pp.66-67, 2004.   과학기술학회마을
20 B. Liu, R. X. Zhang, and L. Wang, Effects of pertussis toxin lectroacupuncture-produced anti-hyperalgesia in inflamed rats. Brain Res. Vol.1044, No.1, pp.87-92, 2005.   DOI   ScienceOn
21 T. Inder, P. S. Huppi, and G. P. Zientara, Early detection of periventricular leukomalacia by diffusion-weighted magnetic resonance imaging techniques. J Pediatr. Vol.134, No.5, pp.631-634, 1999.   DOI   ScienceOn
22 A. E. Baird, A. Benfield, G. Schlaug, and B. Siewert, Enlargement of human cerebral ischemic lesion volumes measured by diffusion-weight. magnetic resonance imaging. Ann Neurol, Vol.41, No.5, pp.581-589, 1997.   DOI   ScienceOn
23 손영주, 정혁상, 구자승, 흰쥐의 족삼리 및 태충전침자극에따른 뇌대사활성의 변화. 대한침구학회지, Vol.19, No.1, pp.159-174, 2002.
24 A. Buffo, C. Rolando, and S. Ceruti, Astrocytes in the damaged brain : Molecular and cellular insights into their reactiv response and healing potential. Biochemical Pharmacology, Vol.99, pp.77-89, 2010.
25 A. Buffo, I. Rite, and P. Tripathi, Origin and progeny of reactive gliosis : a source of multipotent cells in the injured brain. Proc Natl Aca Sci USA. Vol.105, pp.3581-3586, 2008.   DOI   ScienceOn
26 R. S. Cameron and P. Rakic, Glial cell lineage in the cerebral cortex: a review and synthesis. Glia. Vol.4, No.2, pp.124-37, 1991.   DOI
27 M. Pekny and M. Nilsson, Astrocyte activation and reactive gliosis. Glia. Vol.50, pp.427-434, 2005.   DOI   ScienceOn
28 A. Buffo, C. Rolando, and S. Ceruti, Astrocytes in the damaged brain : Molecular and cellular insights into their reactiv response and healing potential. Biochemical Pharmacology. Vol.99, pp.77-89, 2010.
29 A. Buffo, C. Rolando, and S. Ceruti, Astrocytes in the damaged brain : Molecular and cellular insights into their reactiv response and healing potential. Biochemical Pharmacology. Vol.99, pp.77-89, 2010.
30 손인석, 최병태, 장경전. 고빈도 120 Hz 전침이 Carragreenan으로 유발된 흰쥐의 Postaglandin E2와 척수 N-Methyl-D-Aspartate Receptor 발현에 미치는 영향. 대한침구학회지, Vol.20, No.3, pp.15-23, 2003.
31 신홍기, 박동석, 이서은 등. 전침자극이 흰쥐후각 세포의 유해반응에 미치는 효과의 특성, 대한침구학회지, Vol.20, No.3, pp.117-130, 2003.
32 손인석, 최병태, 장경전, 고빈도 120 Hz 전침이 Carragreenan으로 유발된 흰쥐의 Postaglandin E2와 척수 N-Methyl-D-Aspartate Receptor 발현에 미치는 영향. 대한침구학회지. Vol20, No.3, pp.15-23, 2003.
33 S. W. Levison, R. P. Rothstein, and M. J. Romanko, Hypoxia/ischemia depletes the rat perinatal subventricular zone of oligodendrocyte progenitors and neural stem cells. Dev Neurosci. Vol.23, No.3, pp.234-47, 2001.   DOI   ScienceOn
34 신홍기, 박동석, 이서은, 전침자극이 흰쥐후각세포의 유해반응에 미치는 효과의 특성. 대한침구학회지. Vol.20, No.3, pp.117-130, 2003.
35 류성룡, 백용현, 박동석. 전침자극의 Collagen 유발 관절염 모델에 대한 진통효과 및 기전에 관한연구. 대한침구학회지, Vol.23, No.3, pp.77-90, 2006.
36 A. E. Baird, A. Benfield, G. Schlaug, and B. Siewert. Enlargement of human cerebral ischemic lesion volumes measured by diffusion-weight. magnetic resonance imaging. Ann Neurol. Vol.41, No.5, pp.581-589, 1997.   DOI   ScienceOn
37 C. Iadecola, F. Zhang, and R. Casey. Delayed reduction of ischemic brain injury and neurological deficits in mice lacking the inducible nitric oxide synthase gene. J Neurosci. Vol17, pp.9157-9164, 1997.
38 M. Nagayama, F. Zhang, and C. Iadecola, Delayed treatment with aminoguanidine decreases focal cerebral ischemic damage and enhances neurologic recovery in rats. J Cereb
39 N. Tateishi, T. Mori, and Y. Kagamiishi, Astrocytic activation and delayed infarct expansion after permanent focal ischemia in rats. J Cereb Blood Flow Metab. Vol.22, No.6, pp.723-734, 2002.   DOI
40 J. A. Alves, P. Barone, and S. Engelender, Initial stages of radial gliaastrocytic transformation in the early postnatal anterior subventricular zone. J Neurobiol. Vol.52, No.3, pp.251-265, 2002.   DOI   ScienceOn
41 손영주, 정혁상, 구자승, 흰쥐의 족삼리 및 태충전침자극에따른 뇌대사활성의 변화. 대한침구학회지, Vol.19. No.1, pp.159-174, 2002.
42 A. K. Loihl, V. Asensio, and I. L. Campbell, Expression of nitric oxide synthase (NOS)-2 following permanent focal ischemia and the role of nitric oxide in infarct generation in male, female and NOS-2 gene-deficient mice. Brain Res. Vol.830, No.1, pp.155-164, 1999.   DOI   ScienceOn
43 류성룡, 백용현, 박동석. 전침자극의 Collagen 유발 관절염 모델에 대한 진통효과 및 기전에 관한 연구. 대한침구학회지. Vol.23, No.3, pp.77-90, 2006.
44 M. Nagayama, F. Zhang, C. Iadecola, Delayed treatment with aminoguanidine decreases focal cerebral ischemic damage and enhances neurologic recovery in rats. J Cereb Blood Flow Metab. Vol.18, No.10, pp.1107-1113, 1998.   DOI   ScienceOn