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http://dx.doi.org/10.12717/DR.2022.26.2.79

Zebrafish Klf11b is Required to Maintain Cell Viability by Inhibiting p53-Mediated Apoptosis  

Kong, Hee Jeong (Biotechnology Research Division, National Institute of Fisheries Science)
Lee, Jung Jin (Dept. of Chemical and Biological Engineering, Hanbat National University)
Kim, Ju-Won (Biotechnology Research Division, National Institute of Fisheries Science)
Kim, Julan (Biotechnology Research Division, National Institute of Fisheries Science)
Kim, Young-Ok (Biotechnology Research Division, National Institute of Fisheries Science)
Yeo, Sang-Yeob (Dept. of Chemical and Biological Engineering, Hanbat National University)
Publication Information
Development and Reproduction / v.26, no.2, 2022 , pp. 79-90 More about this Journal
Abstract
Krüppel-like factor 10 (KLF10) regulates various cellular functions, such as proliferation, differentiation and apoptosis, as well as the homeostasis of several types of tissue. In the present study, we attempted a loss-of-function analysis of zebrafish Klf11a and Klf11b, which constitute human KLF10 homologs. Embryos injected with klf11b-morpholino (MO) showed developmental retardation and cell death, whereas klf11a-MO-injected embryos showed normal development. In klf11b-MO-injected embryos, a dramatic increase in the amount of zebrafish p53 mRNA might be the cause of the increase in that of bax. The degree of apoptosis decreased in the klf11b-MO and p53-MO co-injected embryos. These findings imply that KLF10 is a negative regulator of p53-dependent transcription, suggesting that the KLF10/p53 complex may play an important role in apoptosis for maintenance of tissue homeostasis during embryonic development.
Keywords
Klf11b; Kruppel-like factor 10 (KLF10); p53; Zebrafish;
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